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Species: Mus musculus
Genetic Insert: Alk.2 shRNA
Vector Backbone Description: Backbone Marker:Addgene Plasmid #11795; Backbone Size:7650; Vector Backbone:pLL3.7; Vector Types:Mammalian Expression, Lentiviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
References:
Comments: Lentiviral vector that expresses shRNA under the mouse U6 promoter. A CMV-EGFP reporter cassette is included in the vector to monitor expression. Supported by the NIAAA INIA West consortium.
Proper citation: RRID:Addgene_59297 Copy
Species: Mus musculus
Genetic Insert: Hrnr
Vector Backbone Description: Backbone Size:7967; Vector Backbone:Brachyury-eGFP; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments: Please note that the final plasmid sequence is post-Gateway recombination. The plasmid contains attB sites (not attR sites as the map implies).
Proper citation: RRID:Addgene_59451 Copy
Species: Mus musculus
Genetic Insert: mLor-IGR3
Vector Backbone Description: Backbone Size:7967; Vector Backbone:Brachyury-eGFP; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments: Please note that the final plasmid sequence is post-Gateway recombination. The plasmid contains attB sites (not attR sites as the map implies).
Proper citation: RRID:Addgene_59454 Copy
Species: Mus musculus
Genetic Insert: mK10-IGR5
Vector Backbone Description: Backbone Size:7967; Vector Backbone:Brachyury-eGFP; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments: Please note that the final plasmid sequence is post-Gateway recombination. The plasmid contains attB sites (not attR sites as the map implies).
Proper citation: RRID:Addgene_59453 Copy
Species: Mus musculus
Genetic Insert: Neuroligin 2 shRNA (rat and mouse)
Vector Backbone Description: Backbone Size:8777; Vector Backbone:pLL3.7; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments: Suitable for knockdown of rat and mouse Nlgn2.
Proper citation: RRID:Addgene_59358 Copy
Species: Mus musculus
Genetic Insert: mSYD1A
Vector Backbone Description: Backbone Size:4015; Vector Backbone:pNICE-HA-N; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: mSYD1A can be released without HA-tag (by SalI / BamHI) or with HA-tag (e.g. by EcoRI / BamHI).
Proper citation: RRID:Addgene_59361 Copy
Species: Mus musculus
Genetic Insert: Nrxn1beta AS4(-)
Vector Backbone Description: Backbone Size:4869; Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: See map & sequence file for insert-releasing enzymes (e.g. NheI / XmaI).
The Nrxn 1 beta variant encoded by this plasmid is AS4(-) / AS5(-):
- Exon 21 constituting alternatively spliced segment 4 (AS4) (=ENSMUSE00001309616) is absent.
- AS5 is absent, i.e. the short form of exon 23 (23.2) is present (=ENSMUSE00001290978; "GTGGGTTAG" missing compared to (longer) exon 23.1 (=ENSMUSE00000862714)).
See Schreiner et al., Neuron, 2014 for nomenclature of alternatively spliced segments (doi:10.1016/j.neuron.2014.09.011).
Proper citation: RRID:Addgene_59409 Copy
Species: Mus musculus
Genetic Insert: FerH-ffLuc2-eGFP
Vector Backbone Description: Backbone Size:5828; Vector Backbone:pFUGW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_71393 Copy
Species: Mus musculus
Genetic Insert: Septin 2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_71547 Copy
Species: Mus musculus
Genetic Insert: Cntn2
Vector Backbone Description: Vector Backbone:pCMVi-SV40ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_71940 Copy
Species: Mus musculus
Genetic Insert: Cntn6
Vector Backbone Description: Vector Backbone:pCMVi-SV40ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_71944 Copy
Species: Mus musculus
Genetic Insert: Cntn4.2
Vector Backbone Description: Vector Backbone:pCMVi-SV40ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_71942 Copy
Species: Mus musculus
Genetic Insert: Flrt1
Vector Backbone Description: Vector Backbone:pCMVi-SV40ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_71947 Copy
Species: Mus musculus
Genetic Insert: Dnmt3a catalytic domain (M. musculus) Dnmt3L (M. musculus) C-terminal domain gene fusion
Vector Backbone Description: Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: For protein production, use BL21 Rosetta (DE3) cells.
Proper citation: RRID:Addgene_71827 Copy
Species: Mus musculus
Genetic Insert: Flrt2
Vector Backbone Description: Vector Backbone:pCMVi-SV40ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_71948 Copy
Species: Mus musculus
Genetic Insert: Cntn1
Vector Backbone Description: Vector Backbone:pCMVi-SV40ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_71939 Copy
Species: Mus musculus
Genetic Insert: Islr
Vector Backbone Description: Vector Backbone:pCMVi-SV40ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_71950 Copy
Species: Mus musculus
Genetic Insert: Islr2.b
Vector Backbone Description: Vector Backbone:pCMVi-SV40ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_71951 Copy
Species: Mus musculus
Genetic Insert: Jam1
Vector Backbone Description: Vector Backbone:pCMVi-SV40ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_71952 Copy
Species: Mus musculus
Genetic Insert: Jam2
Vector Backbone Description: Vector Backbone:pCMVi-SV40ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_71953 Copy
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