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Species: Mus musculus
Genetic Insert: 5" UTR of HSDL2
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:6273; Vector Backbone:psicheck2; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_48180 Copy
Species: Mus musculus
Genetic Insert: 5" UTR of Mut
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:6273; Vector Backbone:psicheck2; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_48164 Copy
Species: Mus musculus
Genetic Insert: 5" UTR of Phb
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:6273; Vector Backbone:psicheck2; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_48162 Copy
Species: Mus musculus
Genetic Insert: 5" UTR of MRPL11
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:6273; Vector Backbone:psicheck2; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_48166 Copy
Species: Mus musculus
Genetic Insert: 5" UTR of Slc25a15
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:6273; Vector Backbone:psicheck2; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_48160 Copy
Species: Mus musculus
Genetic Insert: 5" UTR of Acsm2
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:6273; Vector Backbone:psicheck2; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_48159 Copy
Species: Mus musculus
Genetic Insert: CTS68
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7100; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_48343 Copy
Species: Mus musculus
Genetic Insert: CTS20
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7100; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_48342 Copy
Species: Mus musculus
Genetic Insert: Nrxn1beta AS4(+)
Vector Backbone Description: Backbone Size:4867; Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: See map & sequence file for insert-releasing enzymes (e.g. NheI / XmaI).
The Nrxn 1 beta variant encoded by this plasmid is AS4(+) / AS5(+):
- Exon 21 constituting alternatively spliced segment 4 (AS4) (=ENSMUSE00001309616) is present.
- AS5 is present, i.e. the long form of exon 23 (23.1) is present (=ENSMUSE00000862714; "GTGGGTTAG" in addition to shorter) exon 23.2 (=ENSMUSE00001290978)).
See Schreiner et al., Neuron, 2014 for nomenclature of alternatively spliced segments (doi:10.1016/j.neuron.2014.09.011).
Proper citation: RRID:Addgene_59410 Copy
Species: Mus musculus
Genetic Insert: mKrt14-5IGR
Vector Backbone Description: Backbone Size:7967; Vector Backbone:Brachyury-eGFP; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments: Please note that the final plasmid sequence is post-Gateway recombination. The plasmid contains attB sites (not attR sites as the map implies).
Proper citation: RRID:Addgene_59455 Copy
Species: Mus musculus
Genetic Insert: Alk.1 shRNA
Vector Backbone Description: Backbone Marker:Addgene Plasmid #11795; Backbone Size:7650; Vector Backbone:pLL3.7; Vector Types:Mammalian Expression, Lentiviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
References:
Comments: Lentiviral vector that expresses shRNA under the mouse U6 promoter. A CMV-EGFP reporter cassette is included in the vector to monitor expression. Supported by the NIAAA INIA West consortium.
Proper citation: RRID:Addgene_59296 Copy
Species: Mus musculus
Genetic Insert: Mor2 shRNA
Vector Backbone Description: Backbone Marker:Addgene Plasmid #11795; Backbone Size:7650; Vector Backbone:pLL3.7; Vector Types:Mammalian Expression, Lentiviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
References:
Comments: Lentiviral vector that expresses shRNA under the mouse U6 promoter. A CMV-EGFP reporter cassette is included in the vector to monitor expression. Supported by the NIAAA INIA West consortium.
Proper citation: RRID:Addgene_59298 Copy
Species: Mus musculus
Genetic Insert: Alk.2 shRNA
Vector Backbone Description: Backbone Marker:Addgene Plasmid #11795; Backbone Size:7650; Vector Backbone:pLL3.7; Vector Types:Mammalian Expression, Lentiviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
References:
Comments: Lentiviral vector that expresses shRNA under the mouse U6 promoter. A CMV-EGFP reporter cassette is included in the vector to monitor expression. Supported by the NIAAA INIA West consortium.
Proper citation: RRID:Addgene_59297 Copy
Species: Mus musculus
Genetic Insert: Hrnr
Vector Backbone Description: Backbone Size:7967; Vector Backbone:Brachyury-eGFP; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments: Please note that the final plasmid sequence is post-Gateway recombination. The plasmid contains attB sites (not attR sites as the map implies).
Proper citation: RRID:Addgene_59451 Copy
Species: Mus musculus
Genetic Insert: mLor-IGR3
Vector Backbone Description: Backbone Size:7967; Vector Backbone:Brachyury-eGFP; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments: Please note that the final plasmid sequence is post-Gateway recombination. The plasmid contains attB sites (not attR sites as the map implies).
Proper citation: RRID:Addgene_59454 Copy
Species: Mus musculus
Genetic Insert: mK10-IGR5
Vector Backbone Description: Backbone Size:7967; Vector Backbone:Brachyury-eGFP; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments: Please note that the final plasmid sequence is post-Gateway recombination. The plasmid contains attB sites (not attR sites as the map implies).
Proper citation: RRID:Addgene_59453 Copy
Species: Mus musculus
Genetic Insert: Neuroligin 2 shRNA (rat and mouse)
Vector Backbone Description: Backbone Size:8777; Vector Backbone:pLL3.7; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments: Suitable for knockdown of rat and mouse Nlgn2.
Proper citation: RRID:Addgene_59358 Copy
Species: Mus musculus
Genetic Insert: mSYD1A
Vector Backbone Description: Backbone Size:4015; Vector Backbone:pNICE-HA-N; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: mSYD1A can be released without HA-tag (by SalI / BamHI) or with HA-tag (e.g. by EcoRI / BamHI).
Proper citation: RRID:Addgene_59361 Copy
Species: Mus musculus
Genetic Insert: Nrxn1beta AS4(-)
Vector Backbone Description: Backbone Size:4869; Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: See map & sequence file for insert-releasing enzymes (e.g. NheI / XmaI).
The Nrxn 1 beta variant encoded by this plasmid is AS4(-) / AS5(-):
- Exon 21 constituting alternatively spliced segment 4 (AS4) (=ENSMUSE00001309616) is absent.
- AS5 is absent, i.e. the short form of exon 23 (23.2) is present (=ENSMUSE00001290978; "GTGGGTTAG" missing compared to (longer) exon 23.1 (=ENSMUSE00000862714)).
See Schreiner et al., Neuron, 2014 for nomenclature of alternatively spliced segments (doi:10.1016/j.neuron.2014.09.011).
Proper citation: RRID:Addgene_59409 Copy
Species: Mus musculus
Genetic Insert: HIRA
Vector Backbone Description: Vector Backbone:pcDNA3.1-polyA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_59782 Copy
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