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Species: Homo sapiens
Genetic Insert: human Kv2.1 + HA
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22442077
Proper citation: RRID:Addgene_111525 Copy
Species: Homo sapiens
Genetic Insert: human C73S-Kv2.1 + HA
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22442077
Proper citation: RRID:Addgene_111529 Copy
Species: Homo sapiens
Genetic Insert: human C73L-Kv2.1-HA
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_111527 Copy
Species: Homo sapiens
Genetic Insert: ZF(VEGFA)-StaPL(AI)-YFP-VPR
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCMV-SPORT6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29967496
Comments: [Ref. 1]
NAGAI T., IBATA K., PARK E.S., KUBOTA M., MIKOSHIBA K. & MIYAWAKI A. 2002. A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat Biotechnol 20: 87-90.
[Ref. 2]
CHAVEZ A., SCHEIMAN J., VORA S., PRUITT B.W., TUTTLE M., P R IYER E., LIN S., KIANI S., GUZMAN C.D., WIEGAND D.J., TER-OVANESYAN D., BRAFF J.L., DAVIDSOHN N., HOUSDEN B.E., PERRIMON N., WEISS R., AACH J., COLLINS J.J. & CHURCH G.M. 2015. Highly efficient Cas9-mediated transcriptional programming. Nat Methods 12: 326-328.
Proper citation: RRID:Addgene_111502 Copy
Species: Homo sapiens
Genetic Insert: ZF(VEGFA)-StaPL(TI)-tdRFP-KRAB
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCMV-SPORT6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29967496
Comments: [Ref. 1]
SHANER N.C., CAMPBELL R.E., STEINBACH P.A., GIEPMANS B.N., PALMER A.E. & TSIEN R.Y. 2004. Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nat Biotechnol 22: 1567-1572.
Proper citation: RRID:Addgene_111504 Copy
Species: Homo sapiens
Genetic Insert: VPR-dSpCas9[StaPL(TI)]
Vector Backbone Description: Backbone Marker:System Biosciences; Vector Backbone:pPB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29967496
Comments: [Ref. 1]
SUBACH O.M., GUNDOROV I.S., YOSHIMURA M., SUBACH F.V., ZHANG J., GRUENWALD D., SOUSLOVA E.A., CHUDAKOV D.M., & VERKHUSHA V.V. 2008. Conversion of Red Fluorescent Protein into a Bright Blue Probe. Chem Biol 15 (10):1116-24.
[Ref. 2]
CHAVEZ A., SCHEIMAN J., VORA S., PRUITT B.W., TUTTLE M., P R IYER E., LIN S., KIANI S., GUZMAN C.D., WIEGAND D.J., TER-OVANESYAN D., BRAFF J.L., DAVIDSOHN N., HOUSDEN B.E., PERRIMON N., WEISS R., AACH J., COLLINS J.J. & CHURCH G.M. 2015. Highly efficient Cas9-mediated transcriptional programming. Nat Methods 12: 326-328.
[Ref. 3]
GAO Y., XIONG X., WONG S., CHARLES E.J., LIM W.A. & QI L.S. 2016. Complex transcriptional modulation with orthogonal and inducible dCas9 regulators. Nat Methods 13: 1043-1049.
Proper citation: RRID:Addgene_111508 Copy
Species: Homo sapiens
Genetic Insert: Plasma membrane calcium pump 2, variant x/b
Vector Backbone Description: Backbone Size:5386; Vector Backbone:pcDNA3.1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30174118
Comments: This sequence for PMCA2x/b contains a G73D mutation compared to the reference but the depositing lab confirms that this mutation does not affect plasmid function.
Proper citation: RRID:Addgene_111571 Copy
Species: Homo sapiens
Genetic Insert: PARP1
Vector Backbone Description: Vector Backbone:CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30013063
Proper citation: RRID:Addgene_111575 Copy
Species: Homo sapiens
Genetic Insert: PARP1
Vector Backbone Description: Vector Backbone:pACEBac1; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:30013063
Proper citation: RRID:Addgene_111573 Copy
Species: Homo sapiens
Genetic Insert: ARH3
Vector Backbone Description: Vector Backbone:pET30; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30013063
Proper citation: RRID:Addgene_111578 Copy
Species: Homo sapiens
Genetic Insert: Human heavy chain
Vector Backbone Description: Backbone Marker:ATUM (formerly DNA 2.0); Backbone Size:2673; Vector Backbone:pJ201; Vector Types:Synthetic Biology, Cloning Vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29086492
Proper citation: RRID:Addgene_111567 Copy
Species: Homo sapiens
Genetic Insert: AKIP
Vector Backbone Description: Backbone Marker:Markku Varjosalo, Addgene plasmid #108077; Vector Backbone:MAC-tag-C; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29568061
Comments: CCSB-DFCI hORFeome collection(31022_E10)
Proper citation: RRID:Addgene_111660 Copy
Species: Homo sapiens
Genetic Insert: COX14
Vector Backbone Description: Backbone Marker:Markku Varjosalo, Addgene plasmid #108077; Vector Backbone:MAC-tag-C; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29568061
Comments: CCSB-DFCI hORFeome collection(11040_B05)
Proper citation: RRID:Addgene_111663 Copy
Species: Homo sapiens
Genetic Insert: COX41
Vector Backbone Description: Backbone Marker:Markku Varjosalo, Addgene plasmid #108077; Vector Backbone:MAC-tag-C; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29568061
Comments: CCSB-DFCI hORFeome collection(31028_A04)
Proper citation: RRID:Addgene_111664 Copy
Species: Homo sapiens
Genetic Insert: HXK1
Vector Backbone Description: Backbone Marker:Markku Varjosalo, Addgene plasmid #108077; Vector Backbone:MAC-tag-C; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29568061
Comments: CCSB-DFCI hORFeome collection(11025_D02)
Proper citation: RRID:Addgene_111668 Copy
Species: Homo sapiens
Genetic Insert: EZRI
Vector Backbone Description: Backbone Marker:Markku Varjosalo, Addgene plasmid #108077; Vector Backbone:MAC-tag-C; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29568061
Comments: CCSB-DFCI hORFeome collection(11038_F07)
Proper citation: RRID:Addgene_111665 Copy
Species: Homo sapiens
Genetic Insert: ARP2
Vector Backbone Description: Backbone Marker:Markku Varjosalo, Addgene plasmid #108077; Vector Backbone:MAC-tag-C; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29568061
Comments: CCSB-DFCI hORFeome collection(11035_E02)
Proper citation: RRID:Addgene_111658 Copy
Species: Homo sapiens
Genetic Insert: FLAG-hsDicer
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6264; Vector Backbone:pMSCV-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Comments: Cloning performed in 2 steps: 1) PCR amplification of the original plasmid using primers featuring an overhang containing an XhoI restriction site, 2) Digestion of the pMSCV puro plamid by XhoI and ligation of the insert
Proper citation: RRID:Addgene_111630 Copy
Species: Homo sapiens
Genetic Insert: Exonuclease 1a
Vector Backbone Description: Vector Backbone:pmCherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21808022
Proper citation: RRID:Addgene_111629 Copy
Species: Homo sapiens
Genetic Insert: RAB11A
Vector Backbone Description: Backbone Marker:Markku Varjosalo, Addgene plasmid #108078; Vector Backbone:MAC-tag-N; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29568061
Comments: CCSB-DFCI hORFeome collection(11003_H10)
Proper citation: RRID:Addgene_111691 Copy
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