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Species: Mus musculus
Genetic Insert: Hmga2
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_52038 Copy
Species: Mus musculus
Genetic Insert: Ngn2
Vector Backbone Description: Backbone Size:7745; Vector Backbone:Tet-O-FUW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_52047 Copy
Species: Mus musculus
Genetic Insert: T cell receptor alpha chain
Vector Backbone Description: Backbone Marker:Dario Vignali, Addgene plasmid 52107; Vector Backbone:pMIG II; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: The cDNAs encoding the TCRa (lacking the stop codon) and TCRb chains were linked via a 2A peptide into a single open reading frame by recombinant PCR and cloned into a mouse stem cell virus (MSCV)-based retroviral vector containing an IRES-GFP cassette to facilitate the identification of marked cells.
The 2A peptide remains attached to the TCRa carboxy terminus and can be used to verify the complete separation of the two chains. Inclusion of a Gly-Ser-Gly (GSG) spacer between TCRa and the 2A peptide ensures complete ‘cleavage’. We have not seen any deleterious effects of the 2A peptide on the function of any proteins examined thus far.
Proper citation: RRID:Addgene_52111 Copy
Species: Mus musculus
Genetic Insert: T cell receptor alpha chain
Vector Backbone Description: Backbone Marker:Dario Vignali, Addgene plasmid 52107; Vector Backbone:pMIG II; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: The cDNAs encoding the TCRa (lacking the stop codon) and TCRb chains were linked via a 2A peptide into a single open reading frame by recombinant PCR and cloned into a mouse stem cell virus (MSCV)-based retroviral vector containing an IRES-GFP cassette to facilitate the identification of marked cells.
The 2A peptide remains attached to the TCRa carboxy terminus and can be used to verify the complete separation of the two chains. Inclusion of a Gly-Ser-Gly (GSG) spacer between TCRa and the 2A peptide ensures complete ‘cleavage’. We have not seen any deleterious effects of the 2A peptide on the function of any proteins examined thus far.
Proper citation: RRID:Addgene_52112 Copy
Species: Mus musculus
Genetic Insert: JNK interacting protein-1b (JIP-1b)(471-707)(M1-5)
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_52133 Copy
Species: Mus musculus
Genetic Insert: Estrogen-related receptor beta
Vector Backbone Description: Backbone Marker:Agilent; Backbone Size:4100; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_52188 Copy
Species: Mus musculus
Genetic Insert: Ink4c
Vector Backbone Description: Backbone Marker:Reya lab, Addgene Plasmid 20672; Backbone Size:6573; Vector Backbone:MSCV-IRES-GFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_52220 Copy
Species: Mus musculus
Genetic Insert: TDG
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:6707; Vector Backbone:pTXB3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_52266 Copy
Species: Mus musculus
Genetic Insert: TDG
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:6707; Vector Backbone:pTXB3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_52269 Copy
Species: Mus musculus
Genetic Insert: Dominant negative RFX
Vector Backbone Description: Backbone Size:4798; Vector Backbone:pCAGEN; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Mouse RFX1 DNA binding domain (400-527 aa) with a C-terminal Myc tag
Proper citation: RRID:Addgene_52296 Copy
Species: Mus musculus
Genetic Insert: Arf6
Vector Backbone Description: Backbone Marker:Sigma; Vector Backbone:p3XFLAG-CMV-14; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_52407 Copy
Species: Mus musculus
Genetic Insert: Arf4
Vector Backbone Description: Backbone Marker:Sigma; Vector Backbone:p3XFLAG-CMV-14; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_52405 Copy
Species: Mus musculus
Genetic Insert: Arf1
Vector Backbone Description: Backbone Marker:Sigma; Vector Backbone:p3XFLAG-CMV-14; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_52402 Copy
Species: Mus musculus
Genetic Insert: Mbd3
Vector Backbone Description: Vector Backbone:pBRPy; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_52376 Copy
Species: Mus musculus
Genetic Insert: Mbd3
Vector Backbone Description: Vector Backbone:Fuw; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments: Please note that there are several mismatches in between Addgene's quality control and the depositor's sequence. The mismatches do NOT affect plasmid function.
Proper citation: RRID:Addgene_52373 Copy
Species: Mus musculus
Genetic Insert: Mbd3
Vector Backbone Description: Vector Backbone:Fuw; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments: Please note that there are several mismatches in between Addgene's quality control and the depositor's sequence. The mismatches do NOT affect plasmid function.
Proper citation: RRID:Addgene_52372 Copy
Species: Mus musculus
Genetic Insert: JNK interacting protein-1b (JIP-1b)(471-707) )
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_52428 Copy
Species: Mus musculus
Genetic Insert: p21-p53 Tandem Response Element Promoter
Vector Backbone Description: Backbone Marker:Evrogen; Backbone Size:4127; Vector Backbone:pTurboRFP-PRL; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_52432 Copy
Species: Mus musculus
Genetic Insert: IL-17RA
Vector Backbone Description: Backbone Size:5750; Vector Backbone:pCMV4-Neo; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_46859 Copy
Species: Mus musculus
Genetic Insert: attB, RFP and PGK-puro
Vector Backbone Description: Backbone Size:2977; Vector Backbone:pX1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_46850 Copy
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