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Species: Homo sapiens
Genetic Insert: Rb
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: *CDK mutations (all changed to Ala): T5, S239, S249, T252, T356, T373, S608, S612, S780, S788, S795, S807, S811, T821, T826
Proper citation: RRID:Addgene_58913 Copy
Species: Homo sapiens
Genetic Insert: TEM4
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: Please note that the Addgene verified sequence identified a L722F mutation in TEM4. It is unknown whether this mutation affects protein function.
Proper citation: RRID:Addgene_58895 Copy
Species: Homo sapiens
Genetic Insert: TEM4
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_58894 Copy
Species: Homo sapiens
Genetic Insert: KRAS
Vector Backbone Description: Backbone Marker:unknown; Backbone Size:5169; Vector Backbone:pBabe-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_58899 Copy
Species: Homo sapiens
Genetic Insert: TEM4
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: Please note that the Addgene verified sequence identified a L722F mutation in TEM4. It is unknown whether this mutation affects protein function.
Proper citation: RRID:Addgene_58893 Copy
Species: Homo sapiens
Genetic Insert: hDib1
Vector Backbone Description: Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_58787 Copy
Species: Homo sapiens
Genetic Insert: hDib1
Vector Backbone Description: Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_58788 Copy
Species: Homo sapiens
Genetic Insert: collagen, type IV, alpha 3 binding protein PH domain
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pRS406; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments: PH domain of CERT= Methionine 1 - Glycine 122
Proper citation: RRID:Addgene_58833 Copy
Species: Homo sapiens
Genetic Insert: XIAP
Vector Backbone Description: Vector Backbone:pSil; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_58832 Copy
Species: Homo sapiens
Genetic Insert: Oct4
Vector Backbone Description: Backbone Marker:Dowdy lab; Vector Backbone:T7-VEE-IRES-puro (Addgene plasmid 58970); Vector Types:Mammalian Expression, Venezuelan equine encephalitis (VEE) virus RNA replicon; Bacterial Resistance:Ampicillin
References:
Comments: Summary of features:
1-7561: VEE RNA replicases (nsP1, nsP2, nsP3, nsP4 and 26S promoter, nsP1~4 are nonstructural VEE sequences which encode RNA replicases)
7592-8671: human Oct4
8678-8731: T2A peptide sequence
8738-10147: human Klf4
10154-10213: E2A peptide sequence
10223-11176: human Sox2
11195-11805: IRES sequence
11818-13140: human cMyc
13165-13776: IRES sequence
13777-14376: Puromycin resistance gene
14383-14510:VEE 3’UTR and poly A tail
14679-15539: Ampicillin resistance gene
16319-16336: T7 promoter
Proper citation: RRID:Addgene_58972 Copy
Species: Homo sapiens
Genetic Insert: Oct4
Vector Backbone Description: Backbone Marker:Dowdy lab; Vector Backbone:SP6-VEE-IRES-puro (Addgene plasmid 58971); Vector Types:Mammalian Expression, Venezuelan equine encephalitis (VEE) virus RNA replicon; Bacterial Resistance:Ampicillin
References:
Comments: Summary of features:
1-7561: VEE RNA replicases (nsP1, nsP2, nsP3, nsP4 and 26S promoter, nsP1~4 are nonstructural VEE sequences which encode RNA replicases)
7592-8671: human Oct4
8678-8731: T2A peptide sequence
8738-10147: human Klf4
10154-10213: E2A peptide sequence
10223-11176: human Sox2
11195-11805: IRES sequence
11818-13140: human cMyc
13165-13776: IRES sequence
13777-14376: Puromycin resistance gene
14383-14510:VEE 3’UTR and poly A tail
14679-15539: Ampicillin resistance gene
16320-16337: SP6 promoter
Proper citation: RRID:Addgene_58973 Copy
Species: Homo sapiens
Genetic Insert: Oct4
Vector Backbone Description: Backbone Marker:Dowdy lab; Vector Backbone:SP6-VEE-IRES-puro (Addgene plasmid 58971); Vector Types:Mammalian Expression, Venezuelan equine encephalitis (VEE) virus RNA replicon; Bacterial Resistance:Ampicillin
References:
Comments: Summary of features:
1-7561: VEE RNA replicases (nsP1, nsP2, nsP3, nsP4 and 26S promoter)
7592-8671: human Oct4
8678-8731: T2A peptide sequence
8738-10147: human Klf4
10154-10213: E2A peptide sequence
10223-11176: human Sox2
11195-11805: IRES sequence
11818-13680: human Glis1
13689-14300: IRES sequence
14301-14900: Puromycin resistance gene
14907-15034: VEE 3’UTR and poly A tail
15203-16063: Ampicillin resistance gene
16844-16861: SP6 promoter
Proper citation: RRID:Addgene_58975 Copy
Species: Homo sapiens
Genetic Insert: CAMSAP2
Vector Backbone Description: Vector Backbone:pFasBac; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_59037 Copy
Species: Homo sapiens
Genetic Insert: CAMSAP2 CC+CKK domains
Vector Backbone Description: Vector Backbone:pFasBac; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_59039 Copy
Species: Homo sapiens
Genetic Insert: CAMSAP2 CKK domain
Vector Backbone Description: Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_59032 Copy
Species: Homo sapiens
Genetic Insert: CAMSAP1 CKK domain
Vector Backbone Description: Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_59031 Copy
Species: Homo sapiens
Genetic Insert: CAMSAP2 CC domain
Vector Backbone Description: Vector Backbone:pFasBac; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_59040 Copy
Species: Homo sapiens
Genetic Insert: Rb
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: *CDK mutations (all changed to Ala): T5, S239, S249, T252, T356, T373, S608, S612, S780, S788, S795, S807, S811, T821, T826
Proper citation: RRID:Addgene_58914 Copy
Species: Homo sapiens
Genetic Insert: Rb
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: *CDK mutations (all changed to Ala): T5, S239, S249, T252, T356, T373, S608, S612, S780, S788, S795, S807, S811, T821, T826
Proper citation: RRID:Addgene_58918 Copy
Species: Homo sapiens
Genetic Insert: Rb
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: *CDK mutations (all changed to Ala): T5, S239, S249, T252, T356, T373, S608, S612, S780, S788, S795, S807, S811, T821, T826
Proper citation: RRID:Addgene_58920 Copy
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