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Species: Homo sapiens
Genetic Insert: p53
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5639; Vector Backbone:pET28A; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: Includes a thrombin cleavage site
Proper citation: RRID:Addgene_62083 Copy
Species: Homo sapiens
Genetic Insert: CHRFAM7A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5600; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_62284 Copy
Species: Homo sapiens
Genetic Insert: gp78
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pCDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: *P179L mutation found during Addgene's quality control sequencing is not known to affect function
Proper citation: RRID:Addgene_62370 Copy
Species: Homo sapiens
Genetic Insert: Keap1
Vector Backbone Description: Backbone Size:6470; Vector Backbone:pMIR; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_62459 Copy
Species: Homo sapiens
Genetic Insert: Keap1
Vector Backbone Description: Backbone Size:6470; Vector Backbone:pMIR; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_62457 Copy
Species: Homo sapiens
Genetic Insert: CBX8
Vector Backbone Description: Backbone Marker:SGC (Addgene Plasmid # 26096); Backbone Size:7325; Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: For more information, please see:
http://www.thesgc.org/structures/3i91/
http://www.thesgc.org/structures/5EQ0/
Proper citation: RRID:Addgene_62514 Copy
Species: Homo sapiens
Genetic Insert: CHRFAM7ADelta2bp
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5600; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_62515 Copy
Species: Homo sapiens
Genetic Insert: human Drosha with lysine 382 changed to arginine
Vector Backbone Description: Backbone Marker:clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: Drosha contains M2V, K214R and K380R. Depositor states that these mutation should not affect function.
Proper citation: RRID:Addgene_62519 Copy
Species: Homo sapiens
Genetic Insert: human Drosha with serine 300 changed to alanine
Vector Backbone Description: Backbone Marker:clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: Drosha contains H278R mutation. Depositor states this mutation should not affect function.
Proper citation: RRID:Addgene_62526 Copy
Species: Homo sapiens
Genetic Insert: human Drosha with aa 851-1374 deleted
Vector Backbone Description: Backbone Marker:clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: Drosha contains S195G mutation. Depositor states this mutation should not affect function.
Proper citation: RRID:Addgene_62522 Copy
Species: Homo sapiens
Genetic Insert: human Drosha with aa 1-217 deleted
Vector Backbone Description: Backbone Marker:clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: Drosha contains H278R mutation. Depositor states this mutation should not affect function.
Proper citation: RRID:Addgene_62523 Copy
Species: Homo sapiens
Genetic Insert: human Drosha with aa 1-269 deleted
Vector Backbone Description: Backbone Marker:clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_62524 Copy
Species: Homo sapiens
Genetic Insert: human Drosha with serine 302 changed to aspartic acid
Vector Backbone Description: Backbone Marker:clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: Drosha contains H278R mutation. Depositor states this mutation should not affect function.
Proper citation: RRID:Addgene_62529 Copy
Species: Homo sapiens
Genetic Insert: truncated human Drosha mutant
Vector Backbone Description: Backbone Marker:clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_62532 Copy
Species: Homo sapiens
Genetic Insert: truncated human Drosha mutant
Vector Backbone Description: Backbone Marker:clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_62533 Copy
Species: Homo sapiens
Genetic Insert: human Drosha with serines 300 and 302 changed alanines
Vector Backbone Description: Backbone Marker:clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: Drosha contains H278R. Depositor states that this mutation should not affect function.
Proper citation: RRID:Addgene_62530 Copy
Species: Homo sapiens
Genetic Insert: KRAS
Vector Backbone Description: Backbone Marker:unknown; Backbone Size:5169; Vector Backbone:pBabe-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_58902 Copy
Species: Homo sapiens
Genetic Insert: Rb
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: *CDK mutations (all changed to Ala): T5, S239, S249, T252, T356, T373, S608, S612, S780, S788, S795, S807, S811, T821, T826
Proper citation: RRID:Addgene_58908 Copy
Species: Homo sapiens
Genetic Insert: Rb
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: *CDK mutations (all changed to Ala): T5, S239, S249, T252, T356, T373, S608, S612, S780, S788, S795, S807, S811, T821, T826
Proper citation: RRID:Addgene_58912 Copy
Species: Homo sapiens
Genetic Insert: Rb
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: *CDK mutations (all changed to Ala): T5, S239, S249, T252, T356, T373, S608, S612, S780, S788, S795, S807, S811, T821, T826
Proper citation: RRID:Addgene_58911 Copy
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