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Species: Homo sapiens
Genetic Insert: NM23 H1
Vector Backbone Description: Backbone Size:5558; Vector Backbone:pBABE-hygro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10611312
Proper citation: RRID:Addgene_11366 Copy
Species: Synthetic
Genetic Insert: ncpGCaMP6s
Vector Backbone Description: Backbone Size:3500; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29934970
Proper citation: RRID:Addgene_113674 Copy
Species: Streptococcus pyogenes
Genetic Insert: Cas9
Vector Backbone Description: Backbone Marker:Scott Gradia; Backbone Size:5938; Vector Backbone:pET His6 MBP N10 TEV LIC cloning vector (2C-T); Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29809142
Comments: Cloning of pNS20-SpCas9-SNAP: pMJ922-SpyCas9-GFP bacterial expression vector was a kind gift from Prof. Martin Jinek. GFP was digested using BamHI and KpnI, and SNAPtag-NLS gBlocks (Integrated DNA Technologies) were integrated using In-Fusion HD Cloning Plus CE (Takara).
Proper citation: RRID:Addgene_113717 Copy
Genetic Insert: mutRFP-2A-GFP-IRES-Puro
Vector Backbone Description: Backbone Size:8339; Vector Backbone:Duet011; Vector Types:Mammalian Expression, Lentiviral, Reporter system; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29809142
Comments: Cloning of pNS19-LV-mutRFP-2A-GFP: pEGIP (addgene plasmid #26777) was mutagenized using QuikChange Lightning Multi Site-Directed Mutagenesis Kit (Agilent Technologies) to destroy the start codon of eGFP. Next the vector was linearized with BamHI and In-Fusion HD Cloning Plus CE (Takara) was used to insert the mutRFP-2A gBlocks Gene Fragment (Integrated DNA Technologies).
Proper citation: RRID:Addgene_113716 Copy
Genetic Insert: None
Vector Backbone Description: Vector Backbone:EF.hICN1.CMV.GFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31860685
Comments: The hICN1 insert contains a difference that results in A184S compared to the NCBI reference. This difference does not impact the function of the plasmid.
Proper citation: RRID:Addgene_113715 Copy
Genetic Insert: wbbL
Vector Backbone Description: Vector Backbone:pEXT21; Vector Types:; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:10574995
Comments: Please Note: The plasmid contains two wbbL inserts, which is not known to affect plasmid function.
Proper citation: RRID:Addgene_113662 Copy
Species: humanized monoclonal antibody
Genetic Insert: Emicizumab anti-FX/FXa heavy chain
Vector Backbone Description: Vector Backbone:pcDNA 3.1(+) Hygro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30887655
Proper citation: RRID:Addgene_113665 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ulp1_R1
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29976752
Proper citation: RRID:Addgene_113669 Copy
Vector Backbone Description: Backbone Marker:Genetic Perturbation Platform; Backbone Size:9879; Vector Backbone:pLX_TRC311; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_113668 Copy
Species: Synthetic
Genetic Insert: SaCas9 gRNA Cassete
Vector Backbone Description: Backbone Marker:Alligent Technologies; Vector Backbone:AAV; Vector Types:AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30483052
Comments: The H1TO promoter renders the gRNA inducible via the presence of Doxycycline. See: The Development of a Viral Mediated CRISPR/Cas9 System with Doxycycline Dependent gRNA Expression for Inducible In vitro and In vivo Genome Editing. de Solis CA, Ho A, Holehonnur R, Ploski JE. Front Mol Neurosci. 2016 Aug 18;9:70. doi: 10.3389/fnmol.2016.00070. eCollection 2016. 10.3389/fnmol.2016.00070 PubMed 27587996
Proper citation: RRID:Addgene_113701 Copy
Species: Synthetic
Genetic Insert: SaCas9 gRNA Cassete
Vector Backbone Description: Backbone Marker:Alligent Technologies; Vector Backbone:AAV; Vector Types:AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30483052
Comments: The H1TO promoter renders the gRNA inducible via the presence of Doxycycline. See: The Development of a Viral Mediated CRISPR/Cas9 System with Doxycycline Dependent gRNA Expression for Inducible In vitro and In vivo Genome Editing. de Solis CA, Ho A, Holehonnur R, Ploski JE. Front Mol Neurosci. 2016 Aug 18;9:70. doi: 10.3389/fnmol.2016.00070. eCollection 2016. 10.3389/fnmol.2016.00070 PubMed 27587996
Proper citation: RRID:Addgene_113700 Copy
Genetic Insert: CBF (notch dependent promoter)
Vector Backbone Description: Backbone Size:4403; Vector Backbone:pMuLE ENTR SV40 tdTomato L3-L2; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31860685
Proper citation: RRID:Addgene_113708 Copy
Species: Other
Genetic Insert: ndmB and ndmC
Vector Backbone Description: Vector Backbone:pSB1C3; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31540989
Comments: https://benchling.com/s/kNCaeGXO . Please visit https://www.biorxiv.org/content/early/2018/09/16/419283 for bioRxiv preprint.
Proper citation: RRID:Addgene_113651 Copy
Species: Other
Genetic Insert: promoter T5 and sgRNA
Vector Backbone Description: Backbone Size:2906; Vector Backbone:pYTK095; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30703321
Comments: Please visit https://www.biorxiv.org/content/early/2018/09/16/419283 for bioRxiv preprint.
Proper citation: RRID:Addgene_113655 Copy
Species: Other
Genetic Insert: promoter J23119 and sgRNA
Vector Backbone Description: Backbone Size:2906; Vector Backbone:pYTK095; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30703321
Comments: Please visit https://www.biorxiv.org/content/early/2018/09/16/419283 for bioRxiv preprint.
Proper citation: RRID:Addgene_113654 Copy
Species: Other
Genetic Insert: ndmA and ndmB and ndmC
Vector Backbone Description: Vector Backbone:pSB1C3; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31540989
Comments: https://benchling.com/s/xeoN8t6c . Please visit https://www.biorxiv.org/content/early/2018/09/16/419283 for bioRxiv preprint.
Proper citation: RRID:Addgene_113652 Copy
Vector Backbone Description: Vector Backbone:p6000; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:18944940
Proper citation: RRID:Addgene_113659 Copy
Species: Other
Genetic Insert: TDK gene promoter and dCas9
Vector Backbone Description: Backbone Size:2906; Vector Backbone:pYTK095; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30703321
Comments: Note: This plasmid contains a L64H mutation in Cas9. This mutation is not known to affect plasmid function.
Please visit https://www.biorxiv.org/content/early/2018/09/16/419283 for bioRxiv preprint.
Proper citation: RRID:Addgene_113657 Copy
Species: Other
Genetic Insert: mEGFP-mEGFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2540; Vector Backbone:pDONR221-P4rP3r; Vector Types:CRISPR, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31523744
Comments: Second position vector for three-way Gateway LR reaction to insert C-terminal mEGFP-mEGFP at your locus of interest.
Proper citation: RRID:Addgene_113750 Copy
Species: Other
Genetic Insert: mRuby2-mRuby2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2540; Vector Backbone:pDONR221-P4rP3r; Vector Types:CRISPR, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31523744
Comments: Second position vector for three-way Gateway LR reaction to insert N-terminal mRuby2-mRuby2 at your locus of interest (stop codon removed).
Proper citation: RRID:Addgene_113753 Copy
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