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Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 ORF8 protein
Vector Backbone Description: Backbone Size:6080; Vector Backbone:pTwist_CMV_Puro_6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: NCBI reference sequence: YP_009724396.1
Proper citation: RRID:Addgene_152420 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 nsp8
Vector Backbone Description: Backbone Size:6525; Vector Backbone:pTwist_CMV_Hygro_6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: NCBI reference sequence: YP_009725304.1
Proper citation: RRID:Addgene_152437 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 nsp8
Vector Backbone Description: Backbone Size:4180; Vector Backbone:pTwist_CMV_6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: NCBI reference sequence: YP_009725304.1
Proper citation: RRID:Addgene_152435 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 3'-to-5' exonuclease
Vector Backbone Description: Backbone Size:4180; Vector Backbone:pTwist_CMV_6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: NCBI reference sequence: YP_009725309.1
Proper citation: RRID:Addgene_152439 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 nsp8
Vector Backbone Description: Backbone Size:6525; Vector Backbone:pTwist_CMV_Hygro_6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: NCBI reference sequence: YP_009725304.1
Proper citation: RRID:Addgene_152430 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 nsp9
Vector Backbone Description: Backbone Size:6080; Vector Backbone:pTwist_CMV_Puro_6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: NCBI reference sequence: YP_009725305.1
Proper citation: RRID:Addgene_152434 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: Nsp15
Vector Backbone Description: Backbone Marker:purchased from GenScript; Backbone Size:5644; Vector Backbone:pET-11b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Protein sequence is MHHHHHHSSGVDLGTENLYFQSNAM + Nsp15 (amino acid residues 6453-6798 of YP_009724389).
Used for the determination of the atomic-resolution (2.6 angstrom) structure of the room temperature form of SARS-CoV-2 NendoU by serial femtosecond crystallography at an X-ray free-electron laser (XFEL), Protein Data Bank accession number 7K9P, Jernigan et al 2023 Structure 31:1-14, https://doi.org/10.1016/j.str.2022.12.009, PMID 36630960.
Confers ampicillin-resistance.
Backbone vector is pET-11b from GenScript.
Construct design by Debra T. Hansen.
Proper citation: RRID:Addgene_187656 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: S-GSAS-2P-N317P
Vector Backbone Description: Backbone Size:4746; Vector Backbone:pαH; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_196378 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: Nsp1
Vector Backbone Description: Vector Backbone:EF1-alpha-promoter; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_154071 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 nsp8
Vector Backbone Description: Backbone Size:3069; Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_154758 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 nsp12
Vector Backbone Description: Backbone Size:6610; Vector Backbone:pFastBac; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_154759 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: Spike (HexaPro variant)
Vector Backbone Description: Backbone Size:4746; Vector Backbone:pαH; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_154754 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 ORF6 protein
Vector Backbone Description: Backbone Size:4180; Vector Backbone:pTwist_CMV_6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: NCBI reference sequence: YP_009724394.1
Proper citation: RRID:Addgene_154789 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 ORF7b
Vector Backbone Description: Backbone Size:4180; Vector Backbone:pTwist_CMV_6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: NCBI reference sequence: YP_009725318.1
Proper citation: RRID:Addgene_154796 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 ORF7b
Vector Backbone Description: Backbone Size:6525; Vector Backbone:pTwist_CMV_Hygro_6 with AarI site removed; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: NCBI reference sequence: YP_009725318.1
Proper citation: RRID:Addgene_154797 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 ORF10 protein
Vector Backbone Description: Backbone Size:4180; Vector Backbone:pTwist_CMV_6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: NCBI reference sequence: YP_009725255.1
Proper citation: RRID:Addgene_154798 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 ORF7b
Vector Backbone Description: Backbone Size:4180; Vector Backbone:pTwist_CMV_6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: NCBI reference sequence: YP_009725318.1
Proper citation: RRID:Addgene_154794 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 E (envelope)
Vector Backbone Description: Backbone Size:6080; Vector Backbone:pTwist_CMV_Puro_6 with AarI site removed; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: NCBI reference sequence: YP_009724392.1
Proper citation: RRID:Addgene_154795 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 nsp7
Vector Backbone Description: Backbone Size:6080; Vector Backbone:pTwist_CMV_Puro_6 with AarI site removed; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: NCBI reference sequence: YP_009725303.1
Proper citation: RRID:Addgene_154808 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 ORF7b
Vector Backbone Description: Backbone Size:6525; Vector Backbone:pTwist_CMV_Hygro_6 with AarI site removed; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: NCBI reference sequence: YP_009725318.1
Proper citation: RRID:Addgene_154809 Copy
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