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Species: Homo sapiens
Genetic Insert: Human immunoglobulin Epsilon constant region
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
References:
Comments:
Proper citation: RRID:Addgene_61879 Copy
Species: Homo sapiens
Genetic Insert: HER2/neu receptor specific humanized Gamma 4 heavy chain expression cassette
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
References:
Comments:
Proper citation: RRID:Addgene_61887 Copy
Species: Homo sapiens
Genetic Insert: HER2/neu receptor specific humanized Alpha 1 heavy chain expression cassette
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
References:
Comments:
Proper citation: RRID:Addgene_61888 Copy
Species: Homo sapiens
Genetic Insert: Rab4B
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pAcGFP1-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_61801 Copy
Species: Homo sapiens
Genetic Insert: HER2/neu receptor specific humanized Alpha 2 heavy chain expression cassette
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
References:
Comments:
Proper citation: RRID:Addgene_61889 Copy
Species: Homo sapiens
Genetic Insert: HER2/neu receptor specific humanized Gamma 1 heavy chain expression cassette
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
References:
Comments:
Proper citation: RRID:Addgene_61883 Copy
Species: Homo sapiens
Genetic Insert: HER2/neu receptor specific humanized Gamma 3 heavy chain expression cassette
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
References:
Comments:
Proper citation: RRID:Addgene_61885 Copy
Species: Homo sapiens
Genetic Insert: Rtn4a
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pAcGFP1-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: This plasmid has been found to run as a multimer. Plasmid multimerization often does not impact plasmid function, but may reduce transformation efficiencies. You may need to screen multiple colonies to isolate the monomeric version of this plasmid. If you still have trouble isolating the monomeric version, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.
Proper citation: RRID:Addgene_61807 Copy
Species: Homo sapiens
Genetic Insert: hUCH37 (ISF1)
Vector Backbone Description: Backbone Marker:Invotrogen; Backbone Size:5800; Vector Backbone:pET151-D-TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_61929 Copy
Species: Homo sapiens
Genetic Insert: UBL4A
Vector Backbone Description: Backbone Marker:Origene; Backbone Size:4929; Vector Backbone:pCMV6-ENTRY; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_61809 Copy
Species: Homo sapiens
Genetic Insert: AICDA
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_61926 Copy
Species: Homo sapiens
Genetic Insert: grass pollen allergen Phl p 7 specific human Mu heavy chain expression cassette
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
References:
Comments:
Proper citation: RRID:Addgene_61880 Copy
Species: Homo sapiens
Genetic Insert: USP13
Vector Backbone Description: Backbone Marker:Addgene; Backbone Size:8506; Vector Backbone:pX330-U6-Chimeric_BB-CBh-hSpCas9; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
References:
Comments: Because there is no selective marker in the vector, Western Blot should be used to screen the knockout clones.
Proper citation: RRID:Addgene_61812 Copy
Species: Homo sapiens
Genetic Insert: hUCH37 (ISF1)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5800; Vector Backbone:pET151-D-TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Isoform 2 was created by deletion of E246 by Quickchange mutagenesis from pET151-hUCH37 (ISF1) vector.
Proper citation: RRID:Addgene_61930 Copy
Species: Homo sapiens
Genetic Insert: gp78
Vector Backbone Description: Backbone Marker:Addgene; Backbone Size:8506; Vector Backbone:pX330-U6-Chimeric_BB-CBh-hSpCas9; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
References:
Comments: Because there is no selective marker, Western blot should be used to screen the knockout clones.
Proper citation: RRID:Addgene_61833 Copy
Species: Homo sapiens
Genetic Insert: cyclin A
Vector Backbone Description: Backbone Marker:Pines Lab; Vector Backbone:pt7 venus cks1 (1843); Vector Types:in vitro transcription; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_61839 Copy
Species: Homo sapiens
Genetic Insert: cyclin A
Vector Backbone Description: Backbone Marker:Amersham; Vector Backbone:pGEX 6P1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_61844 Copy
Species: Homo sapiens
Genetic Insert: cyclin B1
Vector Backbone Description: Backbone Marker:unknown; Vector Backbone:pcDNA5/FRT/TO/STREP 3x FLAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_61841 Copy
Species: Homo sapiens
Genetic Insert: cyclin A
Vector Backbone Description: Backbone Marker:unknown; Vector Backbone:pCeru-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_61842 Copy
Species: Homo sapiens
Genetic Insert: cdc20
Vector Backbone Description: Backbone Marker:Pines Lab; Vector Backbone:pcDNA5/FRT/TO(Neo)-Venus-Flag; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_61848 Copy
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