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Species: Mus musculus
Genetic Insert: PDZD7
Vector Backbone Description: Backbone Marker:Vector Builder ; Backbone Size:3855; Vector Backbone:pRP[Exp]-CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_83198 Copy
Species: Mus musculus
Genetic Insert: FoxO1
Vector Backbone Description: Backbone Marker:Invivogen; Backbone Size:3390; Vector Backbone:pSELECT-puro; Vector Types:Mammalian Expression; Bacterial Resistance:None
References:
Comments: To construct pSELECT-HA-mFOXO1 the coding sequence of HA tag and FOXO1 were amplified from pCMV-HA-FOXO1 by PCR. The PCR product was digested with NheI and SalI restriction enzymes and inserted into the NheI and SalI sites of pSELECT-puro.
The insert was sequenced and compared to mouse FOXO1 (NM_019739.3). Sequence identity was confirmed except for a conservative A->G mutation at base 474, a non-conservative A->G mutation at base 1121 and a non-conservative mutation T->C mutation at base 2321 of NM_019739.3. These mutations were confirmed to exist in the original pCMV-HA-FOXO1 plasmid. The non-conservative mutations result in a K->R substitution at amino acid 219 and a L->P substitution at amino acid 619 of mFOXO1 (NP_062713.2).
Mutations at bases 1121 and 2321 were reversed to wild type by site-directed mutagenesis and the corrections were confirmed by sequencing
Proper citation: RRID:Addgene_83308 Copy
Species: Mus musculus
Genetic Insert: Stat5b
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pAD/CMV/V5-DEST; Vector Types:Adenoviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_83262 Copy
Species: Mus musculus
Genetic Insert: Stat5A
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pAD/CMV/V5-DEST; Vector Types:Adenoviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_83253 Copy
Species: Mus musculus
Genetic Insert: Stat5a
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pAD/CMV/V5-DEST; Vector Types:Adenoviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_83252 Copy
Species: Mus musculus
Genetic Insert: TRIOBP
Vector Backbone Description: Backbone Marker:Vector Builder ; Backbone Size:3855; Vector Backbone:pRP[Exp]-CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_83201 Copy
Species: Mus musculus
Genetic Insert: SLC9A3R2
Vector Backbone Description: Backbone Marker:Vector Builder ; Backbone Size:3855; Vector Backbone:pRP[Exp]-CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_83200 Copy
Species: Mus musculus
Genetic Insert: Rab21
Vector Backbone Description: Backbone Size:4681; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_83422 Copy
Species: Mus musculus
Genetic Insert: Rab21
Vector Backbone Description: Backbone Size:4681; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_83421 Copy
Species: Mus musculus
Genetic Insert: Talin1
Vector Backbone Description: Vector Backbone:pLPCX; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: XhoI RE site in the vector backbone is disrupted. Insert also contains ClaI site.
F40 refers to the 40-aa peptide (GPGGA)8 derived from the elastic spider silk protein flagelliform
Proper citation: RRID:Addgene_83377 Copy
Species: Mus musculus
Genetic Insert: HGF
Vector Backbone Description: Vector Backbone:WAP-SKII (pBluescript SKII); Vector Types:Mouse Targeting; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_83503 Copy
Species: Mus musculus
Genetic Insert: CALR
Vector Backbone Description: Vector Backbone:pmcsg7; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_83506 Copy
Species: Mus musculus
Genetic Insert: CALR
Vector Backbone Description: Vector Backbone:pmcsg7; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_83508 Copy
Species: Mus musculus
Genetic Insert: polycystin-1
Vector Backbone Description: Vector Backbone:pcDNA4/TO/2xMyc/His; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_83453 Copy
Species: Mus musculus
Genetic Insert: polycystin-1
Vector Backbone Description: Vector Backbone:pcDNA4/TO/2xMyc/His; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_83452 Copy
Species: Mus musculus
Genetic Insert: mouse Fc epsilon RI gamma cDNA
Vector Backbone Description: Backbone Marker:Pharmacia; Backbone Size:4889; Vector Backbone:pSVL; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_8372 Copy
Species: Mus musculus
Genetic Insert: MYRF
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:7791; Vector Backbone:pLVX-Tight-Puro; Vector Types:Mammalian Expression, Bacterial Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_64843 Copy
Species: Mus musculus
Genetic Insert: lnc-BATE1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6600; Vector Backbone:pSIREN-RetroQZsGreen; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_64864 Copy
Species: Mus musculus
Genetic Insert: Akt2
Vector Backbone Description: Backbone Size:8892; Vector Backbone:pLVX-IRES-tdTomato; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_64832 Copy
Species: Mus musculus
Genetic Insert: Akt2
Vector Backbone Description: Backbone Size:8892; Vector Backbone:pLVX-IRES-tdTomato; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_64834 Copy
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