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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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|---|---|---|---|---|---|---|---|---|---|---|
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pMXs-B1 Resource Report Resource Website |
RRID:Addgene_31705 | miR-106a | Mus musculus | Ampicillin | PMID:21454525 | Backbone Size:4600; Vector Backbone:pMXs; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | 2026-07-25 12:45:45 | 0 | ||
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pNLS-LSSmKate1-N1 Resource Report Resource Website |
RRID:Addgene_31870 | NLS | Mus musculus | Kanamycin | PMID:20212155 | Backbone Marker:Clontech; Backbone Size:4678; Vector Backbone:N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | K69Y/P131T/S148G/M167E/T183S/M196V compared to mKate but numbering is relative to EGFP | 2026-07-25 12:45:46 | 0 | |
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pTight-9-124 Resource Report Resource Website 1+ mentions |
RRID:Addgene_31874 | miR-9/9* and miR-124 | Mus musculus | Ampicillin | PMID:21753754 | Addgene's quality control sequence is missing the following sequence upstream of IRES - "CCGCAAATT". This deletion should not affect plasmid function. | Backbone Marker:home made/Crabtree lab; Vector Backbone:pTLemiR; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-07-25 12:45:46 | 3 | |
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psiCHECK-2-nMyc-m2_3'UTR Resource Report Resource Website |
RRID:Addgene_31860 | nMyc mutant-1 3'UTR | Mus musculus | Ampicillin | PMID:20054295 | Please note that there are 3 additional point mutations (in addition to the mutated seed sequence). The additional mutations should not affect plasmid function. | Backbone Size:6273; Vector Backbone:psiCHECK-2; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin | Second Let-7c Seed changed from TACCTC to TAACGC | 2026-07-25 12:45:46 | 0 |
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psiCHECK-2-Sal4-wt_3'UTR Resource Report Resource Website 1+ mentions |
RRID:Addgene_31862 | Sal4 wild-type 3'UTR | Mus musculus | Ampicillin | PMID:20054295 | Backbone Size:6273; Vector Backbone:psiCHECK-2; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin | 2026-07-25 12:45:46 | 1 | ||
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pcDNA3.1/HisC-mTAZ Resource Report Resource Website 1+ mentions |
RRID:Addgene_31793 | TAZ | Mus musculus | Ampicillin | PMID:16099986 | Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1/His C; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-07-25 12:45:45 | 2 | ||
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pT3-myr-AKT-HA Resource Report Resource Website 10+ mentions |
RRID:Addgene_31789 | Akt1 | Mus musculus | Ampicillin | PMID:21147110 | This plasmid is in the pT3-EF1a vector which contains loxP sites flanking the inverted repeats of SB (sleeping beauty) sequence. Therefore this plasmid cannot not used with Cre for in vivo studies. | Backbone Size:7000; Vector Backbone:pT3-EF1a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-07-25 12:45:45 | 42 | |
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MSCV-ERK5-AEF Resource Report Resource Website |
RRID:Addgene_31816 | Erk5 | Mus musculus | Ampicillin | PMID:16166637 | ERK5-AEF was added by shuttling the NheI(blunted)-NotI cDNA fragments from a previously described pCI plasmid construct (Kasler et al, 2000) into pBS-IRES and then cloning the NotI fragments into the NotI site of the MSCV-puro vector. Addgene sequencing results found an additional L->V point mutation at residue 188. This is a conservative change and depositor says this should not alter the function of this construct. | Backbone Marker:Clontech; Backbone Size:6300; Vector Backbone:pMSCVpuro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | T219A and Y221F | 2026-07-25 12:45:46 | 0 |
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pLKO-puro FLAG wildtype, catalytic active lipin 1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_32010 | lipin1 | Mus musculus | Ampicillin | PMID:21816276 | Backbone Size:7100; Vector Backbone:pLKO.1-puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | 2026-07-25 12:45:47 | 1 | ||
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myc-mULK1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_31960 | ULK1 | Mus musculus | Ampicillin | PMID:19225151 | Backbone Size:4754; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-07-25 12:45:47 | 6 | ||
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pLKO-puro FLAG SREBP1 Resource Report Resource Website 10+ mentions |
RRID:Addgene_32017 | SREBP1 | Mus musculus | Ampicillin | PMID:21816276 | Backbone Size:7100; Vector Backbone:pLKO.1-puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | V225M | 2026-07-25 12:45:47 | 15 | |
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HA-mULK1M92A Resource Report Resource Website |
RRID:Addgene_31964 | ULK1 | Mus musculus | Ampicillin | PMID:19225151 | Backbone Size:4754; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | M92A | 2026-07-25 12:45:47 | 0 | |
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pRK5 FLAG wildtype, catalytic active lipin 1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_32005 | lipin1 | Mus musculus | Ampicillin | PMID:21816276 | Backbone Size:4700; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-07-25 12:45:47 | 6 | ||
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pRK5 FLAG wildtype, catalytic dead lipin 1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_32006 | Lipin1 | Mus musculus | Ampicillin | PMID:21816276 | Backbone Size:4700; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | D712E and D714E | 2026-07-25 12:45:47 | 1 | |
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pRK5 FLAG 17xS/T->A, catalytic active lipin 1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_32007 | lipin1 | Mus musculus | Ampicillin | PMID:21816276 | The 17xS/T→ A mutant encompasses the following residues: S106, S150, (S281, T282), S285, S287, S293, T298, S328, (S353, S356), S392, S468, S472, S483, S634, S635, (S647, S648). Residues in parentheses were both mutated to alanine because the singly phosphorylated site in each case could not be unambiguously determined by mass spectrometry S921A, S923A were previously reported to have been present in this plasmid, but these residues are in fact WT. This change should no affect the reported function. | Backbone Size:4700; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | S106A, S150A, (S281A, T282A), S285A, S287A, S293A, T298A, S328A, (S353A, S356A), S392A, S468A, S472A, S483A, S634A, S635A, (S647A, S648A) | 2026-07-25 12:45:47 | 2 |
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pRK5 FLAG 17xS/T->A, catalytic dead lipin 1 Resource Report Resource Website |
RRID:Addgene_32008 | Lipin1 | Mus musculus | Ampicillin | PMID:21816276 | The 17xS/T→ A mutant encompasses the following residues: S106, S150, (S281, T282), S285, S287, S293, T298, S328, (S353, S356), S392, S468, S472, S483, S634, S635, (S647, S648). Residues in parentheses were both mutated to alanine because the singly phosphorylated site in each case could not be unambiguously determined by mass spectrometry S921A, S923A were previously reported to have been present in this plasmid, but these residues are in fact WT. This change should no affect the reported function. | Backbone Size:4700; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | D712E, D714E AND S106A, S150A, (S281A, T282A), S285A, S287A, S293A, T298A, S328A, (S353A, S356A), S392A, S468A, S472A, S483A, S634A, S635A, (S647A, S648A) | 2026-07-25 12:45:47 | 0 |
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pLKO-puro FLAG SREBP2 Resource Report Resource Website 10+ mentions |
RRID:Addgene_32018 | SREBP2 | Mus musculus | Ampicillin | PMID:21816276 | Backbone Size:7100; Vector Backbone:pLKO.1-puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | 2026-07-25 12:45:47 | 11 | ||
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pMAL-c2x-MT3 Resource Report Resource Website |
RRID:Addgene_32115 | MT (3x) | Mus musculus | Ampicillin | PMID:19114107 | In this construct, a translational modification was made that changes an alanine to an aspartic acid at the junction between MT genes. The third copy of the MT gene was inserted into the pMAL-c2x-MT2 plasmid (Addgene plasmids 32102) before the existing MT copies. The inserted MT gene was amplified from pET-3d-MT using the forward primer (50-CTCGGGATCGAGGGAAGGATTTCAAGATATACCATGGACCCC-30), which codes for the MBP linker region containing an XmnI and NcoI site fused in frame to the MT gene, and the reverse primer (50-GTGACCACATGTCACAGCACGTGCACTTGTCC-3 0 ), which contains an AflIII site at the MT–MT junction. The pMAL-c2x-MT2 plasmid was prepared by digesting with XmnI and NcoI, and the PCR product was constructed with XmnI and AflIII. Since NcoI and AflIII produced equivalent DNA ends, the second copy of MT was inserted with removal of both restriction sites at the ligation junction. This process can be reproduced iteratively to introduce as many copies of MT in frame into the construct as desired. In addition to this plasmid Addgene offers versions with 1 copy (32101), 2 copies (32102) and 4 copies (32100) of MT. | Backbone Marker:New England Biolabs; Backbone Size:6700; Vector Backbone:pMAL-c2x; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-07-25 12:45:48 | 0 | |
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pMAL-c2x-MT2 Resource Report Resource Website 1+ mentions |
RRID:Addgene_32102 | MT (2x) | Mus musculus | Ampicillin | PMID:17692533 | In this construct, a translational modification was made that changes an alanine to an aspartic acid at the junction between the two MT genes. The second copy of the MT gene was inserted into the pMAL-c2x-MT1 plasmid (Addgene plasmids 32101) before the existing MT copy. The inserted MT gene was amplified from pET-3d-MT using the forward primer (50-CTCGGGATCGAGGGAAGGATTTCAAGATATACCATGGACCCC-30), which codes for the MBP linker region containing an XmnI and NcoI site fused in frame to the MT gene, and the reverse primer (50-GTGACCACATGTCACAGCACGTGCACTTGTCC-3 0 ), which contains an AflIII site at the MT–MT junction. The pMAL-c2x-MT plasmid was prepared by digesting with XmnI and NcoI, and the PCR product was constructed with XmnI and AflIII. Since NcoI and AflIII produced equivalent DNA ends, the second copy of MT was inserted with removal of both restriction sites at the ligation junction. This process can be reproduced iteratively to introduce as many copies of MT in frame into the construct as desired. In addition to this plasmid Addgene offers versions with 1 copy (32101), 3 copies (32115) and 4 copies (32100) of MT. | Backbone Marker:New England Biolabs; Backbone Size:6700; Vector Backbone:pMAL-c2x; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-07-25 12:45:48 | 1 | |
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1099-N-cadherin-AAA-YFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_32237 | N-cadherin | Mus musculus | Kanamycin | PMID:14610057 | Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | Glu–Glu–Asp (aa 780–782) ---> Ala–Ala–Ala | 2026-07-25 12:45:49 | 1 |
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