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Species: Homo sapiens
Genetic Insert: OTUD3
Vector Backbone Description: Backbone Marker:OPPF-UK; Backbone Size:6299; Vector Backbone:pOPINK; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: Hospenthal MK, Mevissen TET, Komander D. Nature Protoc. 2015 Jan 29;10:349-361. doi:10.1038/nprot.2015.018
Proper citation: RRID:Addgene_61412 Copy
Species: Homo sapiens
Genetic Insert: TRAIL receptor 2
Vector Backbone Description: Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_61383 Copy
Species: Homo sapiens
Genetic Insert: TRAIL receptor 4
Vector Backbone Description: Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_61385 Copy
Species: Homo sapiens
Genetic Insert: TRAIL receptor 3
Vector Backbone Description: Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_61384 Copy
Species: Homo sapiens
Genetic Insert: Inhibitor-3
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP_C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: resistant against siRNA oligomer I3 S2 (CUGCUGTAUUUAUGAGAAA)
Proper citation: RRID:Addgene_61399 Copy
Species: Homo sapiens
Genetic Insert: CenpB-GFP-Halo
Vector Backbone Description: Backbone Marker:Dr. Eugene Makeyev; Vector Backbone:pEM791; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_61502 Copy
Species: Homo sapiens
Genetic Insert: HaloTag-GFP-Mito
Vector Backbone Description: Backbone Marker:Dr. Eugene Makeyev; Vector Backbone:pEM791; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_61500 Copy
Species: Homo sapiens
Genetic Insert: HaloTag-GFP-PACT
Vector Backbone Description: Backbone Marker:Dr. Eugene Makeyev; Vector Backbone:pEM791; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_61501 Copy
Species: Homo sapiens
Genetic Insert: PK-hLC3∆G
Vector Backbone Description: Backbone Size:9000; Vector Backbone:FUGW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments: We thank Dr. James Edward Rothman for providing respective pHluorin.
Proper citation: RRID:Addgene_61461 Copy
Species: Homo sapiens
Genetic Insert: NEAT1
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:4000; Vector Backbone:PCRII; Vector Types:general cloning vector; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments: *To create this plasmid, hNEAT1 was amplified from HeLa cDNA. The insert contains two mismatches (C->T at bp 78, and A->G at bp 91, an A->G at bp 1666, and a deletion of TA at bp 2031 and 2032 using the numbering of NR_028272.1) compared to the canonical hNEAT1 sequence. These variants are likely to be encoded in the HeLa genome, as they appeared in multiple clones, but this is yet to be verified by sequencing of HeLa genomic DNA.
Proper citation: RRID:Addgene_61518 Copy
Species: Homo sapiens
Genetic Insert: Ngn1
Vector Backbone Description: Backbone Marker:Lab of David Baltimore; Vector Backbone:pFUGW; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_61473 Copy
Species: Homo sapiens
Genetic Insert: RAB14 3' UTR
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:7350; Vector Backbone:pmirGLO Dual-Luciferase; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_61489 Copy
Species: Homo sapiens
Genetic Insert: FK506 binding protein 1A
Vector Backbone Description: Vector Backbone:C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: T429E in AIP following (in-frame with) FKBP caused by GA mismatch at 1313 bp, AC mismatch at 1314 bp, and AT mismatch at 1315 bp in Author's partial sequence (please refer to Tom20-mChF-AIP for comparison).
Proper citation: RRID:Addgene_61522 Copy
Species: Homo sapiens
Genetic Insert: FK506 binding protein 1A
Vector Backbone Description: Vector Backbone:C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: T389A in AIP following (in-frame with) FKBP caused by GA mismatch at 1180 bp and CT mismatch at 1182 bp in Author's partial sequence (please refer to mChF-AIP for comparison).
Proper citation: RRID:Addgene_61528 Copy
Species: Homo sapiens
Genetic Insert: MEF2CA
Vector Backbone Description: Backbone Size:8376; Vector Backbone:TetO-FUW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_61538 Copy
Species: Homo sapiens
Genetic Insert: HaloTag-GFP-Nuf2
Vector Backbone Description: Backbone Marker:Dr. Eugene Makeyev; Vector Backbone:pEM791; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_61499 Copy
Species: Homo sapiens
Genetic Insert: REST
Vector Backbone Description: Backbone Size:8376; Vector Backbone:TetO-FUW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_61533 Copy
Species: Homo sapiens
Genetic Insert: mir124a
Vector Backbone Description: Backbone Size:8376; Vector Backbone:TetO-FUW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_61539 Copy
Species: Homo sapiens
Genetic Insert: FK506 binding protein 1A
Vector Backbone Description: Vector Backbone:C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: T389E in AIP (in-frame with) FKBP caused by GA mismatch at 1180 bp, AC mismatch at 1181 bp, and AT mismatch at 1182 bp in Author's partial sequence (please refer to mChF-AIP for comparison).
Proper citation: RRID:Addgene_61530 Copy
Species: Homo sapiens
Genetic Insert: RAB14 3' UTR
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:7350; Vector Backbone:pmirGLO Dual-Luciferase; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments: Oligos used for site-directed mutagenesis:
F-ATTACAGGCTGCATTCTTGCGAAAAAGAAAAAAGGCTGGG
R-CCCAGCCTTTTTTCTTTTTCGCAAGAATGCAGCCTGTAAT
Proper citation: RRID:Addgene_61490 Copy
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