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Species: Mus musculus
Genetic Insert: miR-106a
Vector Backbone Description: Backbone Size:4600; Vector Backbone:pMXs; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_31705 Copy
Species: Mus musculus
Genetic Insert: NLS
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4678; Vector Backbone:N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_31870 Copy
Species: Mus musculus
Genetic Insert: miR-9/9* and miR-124
Vector Backbone Description: Backbone Marker:home made/Crabtree lab; Vector Backbone:pTLemiR; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments: Addgene's quality control sequence is missing the following sequence upstream of IRES - "CCGCAAATT". This deletion should not affect plasmid function.
Proper citation: RRID:Addgene_31874 Copy
Species: Mus musculus
Genetic Insert: nMyc mutant-1 3'UTR
Vector Backbone Description: Backbone Size:6273; Vector Backbone:psiCHECK-2; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments: Please note that there are 3 additional point mutations (in addition to the mutated seed sequence). The additional mutations should not affect plasmid function.
Proper citation: RRID:Addgene_31860 Copy
Species: Mus musculus
Genetic Insert: Sal4 wild-type 3'UTR
Vector Backbone Description: Backbone Size:6273; Vector Backbone:psiCHECK-2; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_31862 Copy
Species: Mus musculus
Genetic Insert: TAZ
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1/His C; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_31793 Copy
Species: Mus musculus
Genetic Insert: Akt1
Vector Backbone Description: Backbone Size:7000; Vector Backbone:pT3-EF1a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: This plasmid is in the pT3-EF1a vector which contains loxP sites flanking the inverted repeats of SB (sleeping beauty) sequence. Therefore this plasmid cannot not used with Cre for in vivo studies.
Proper citation: RRID:Addgene_31789 Copy
Species: Mus musculus
Genetic Insert: Erk5
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6300; Vector Backbone:pMSCVpuro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: ERK5-AEF was added by shuttling the NheI(blunted)-NotI cDNA fragments from a previously described pCI plasmid construct (Kasler et al, 2000) into pBS-IRES and then cloning the NotI fragments into the NotI site of the MSCV-puro vector.
Addgene sequencing results found an additional L->V point mutation at residue 188. This is a conservative change and depositor says this should not alter the function of this construct.
Proper citation: RRID:Addgene_31816 Copy
Species: Mus musculus
Genetic Insert: lipin1
Vector Backbone Description: Backbone Size:7100; Vector Backbone:pLKO.1-puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_32010 Copy
Species: Mus musculus
Genetic Insert: ULK1
Vector Backbone Description: Backbone Size:4754; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_31960 Copy
Species: Mus musculus
Genetic Insert: SREBP1
Vector Backbone Description: Backbone Size:7100; Vector Backbone:pLKO.1-puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_32017 Copy
Species: Mus musculus
Genetic Insert: ULK1
Vector Backbone Description: Backbone Size:4754; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_31964 Copy
Species: Mus musculus
Genetic Insert: lipin1
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_32005 Copy
Species: Mus musculus
Genetic Insert: Lipin1
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_32006 Copy
Species: Mus musculus
Genetic Insert: lipin1
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: The 17xS/T→ A mutant encompasses the following residues: S106, S150, (S281, T282), S285, S287, S293, T298, S328, (S353, S356), S392, S468, S472, S483, S634, S635, (S647, S648). Residues in parentheses were both mutated to alanine because the singly phosphorylated site in each case could not be unambiguously determined by mass spectrometry
S921A, S923A were previously reported to have been present in this plasmid, but these residues are in fact WT. This change should no affect the reported function.
Proper citation: RRID:Addgene_32007 Copy
Species: Mus musculus
Genetic Insert: Lipin1
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: The 17xS/T→ A mutant encompasses the following residues: S106, S150, (S281, T282), S285, S287, S293, T298, S328, (S353, S356), S392, S468, S472, S483, S634, S635, (S647, S648). Residues in parentheses were both mutated to alanine because the singly phosphorylated site in each case could not be unambiguously determined by mass spectrometry
S921A, S923A were previously reported to have been present in this plasmid, but these residues are in fact WT. This change should no affect the reported function.
Proper citation: RRID:Addgene_32008 Copy
Species: Mus musculus
Genetic Insert: SREBP2
Vector Backbone Description: Backbone Size:7100; Vector Backbone:pLKO.1-puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_32018 Copy
Species: Mus musculus
Genetic Insert: MT (3x)
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:6700; Vector Backbone:pMAL-c2x; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: In this construct, a translational modification was made that changes an alanine to an aspartic acid at the junction between MT genes. The third copy of the MT gene was inserted into the pMAL-c2x-MT2 plasmid (Addgene plasmids 32102) before the existing MT copies. The inserted MT gene was amplified from pET-3d-MT using the forward primer (50-CTCGGGATCGAGGGAAGGATTTCAAGATATACCATGGACCCC-30), which codes for the MBP linker region containing an XmnI and NcoI site fused in frame to the MT gene, and the reverse primer (50-GTGACCACATGTCACAGCACGTGCACTTGTCC-3 0 ), which contains an AflIII site at the MT–MT junction. The pMAL-c2x-MT2 plasmid was prepared by digesting with XmnI and NcoI, and the PCR product was constructed with XmnI and AflIII. Since NcoI and AflIII produced equivalent DNA ends, the second copy of MT was inserted with removal of both restriction sites at the ligation junction. This process can be reproduced iteratively to introduce as many copies of MT in frame into the construct as desired. In addition to this plasmid Addgene offers versions with 1 copy (32101), 2 copies (32102) and 4 copies (32100) of MT.
Proper citation: RRID:Addgene_32115 Copy
Species: Mus musculus
Genetic Insert: MT (2x)
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:6700; Vector Backbone:pMAL-c2x; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: In this construct, a translational modification was made that changes an alanine to an aspartic acid at the junction between the two MT genes. The second copy of the MT gene was inserted into the pMAL-c2x-MT1 plasmid (Addgene plasmids 32101) before the existing MT copy. The inserted MT gene was amplified from pET-3d-MT using the forward primer (50-CTCGGGATCGAGGGAAGGATTTCAAGATATACCATGGACCCC-30), which codes for the MBP linker region containing an XmnI and NcoI site fused in frame to the MT gene, and the reverse primer (50-GTGACCACATGTCACAGCACGTGCACTTGTCC-3 0 ), which contains an AflIII site at the MT–MT junction. The pMAL-c2x-MT plasmid was prepared by digesting with XmnI and NcoI, and the PCR product was constructed with XmnI and AflIII. Since NcoI and AflIII produced equivalent DNA ends, the second copy of MT was inserted with removal of both restriction sites at the ligation junction. This process can be reproduced iteratively to introduce as many copies of MT in frame into the construct as desired. In addition to this plasmid Addgene offers versions with 1 copy (32101), 3 copies (32115) and 4 copies (32100) of MT.
Proper citation: RRID:Addgene_32102 Copy
Species: Mus musculus
Genetic Insert: N-cadherin
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_32237 Copy
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