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Species: Homo sapiens
Genetic Insert: Parkin
Vector Backbone Description: Backbone Size:4800; Vector Backbone:pRK5-Myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_17612 Copy
Species: Homo sapiens
Genetic Insert: Parkin
Vector Backbone Description: Backbone Size:4800; Vector Backbone:pRK5-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_17613 Copy
Species: Homo sapiens
Genetic Insert: EF-1a promoter
Vector Backbone Description: Backbone Marker:Dr. Didier Trono; Backbone Size:0; Vector Backbone:SIN LV pRLLhPGK.GFP Sin-18 (modified); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments: SIN lentiviral vector that expresses GFP controlled by the promoter of the housekeeping gene EF-1alpha.
We constructed EF.GFP by replacing the human PGK promoter (EcoRV to BamHI sites in the PGK.GFP vector) with human EF-1alpha promoter from plasmid pEF1/Myc-His-A (Invitrogen, Carlsbad, CA). The EF-1alpha promoter (nucleotide 373 to 1560 as numbered in GenBank accession J04616) contains the first exon, first intron, and part of the second exon before the coding sequence (starting at nucleotide 1582).
Recombinant LVs were generated using the 3-plasmid system by cotransfection of 293T cells through calcium phosphate precipitation. The ratio of a transducing vector, pMD.G, and pCMVDeltaR8.91 was fixed at 1.5:0.5:2 µg for 10^6 293T cells.
Proper citation: RRID:Addgene_17616 Copy
Species: Homo sapiens
Genetic Insert: TRIO
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_176111 Copy
Species: Homo sapiens
Genetic Insert: ITSN1
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: Please visit https://doi.org/10.1101/2022.10.17.512253 for bioRxiv preprint.
Proper citation: RRID:Addgene_176113 Copy
Species: Homo sapiens
Genetic Insert: RHOGEF p63
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: Please visit https://doi.org/10.1101/2022.10.17.512253 for bioRxiv preprint.
Proper citation: RRID:Addgene_176112 Copy
Species: Homo sapiens
Genetic Insert: PBZ-C
Vector Backbone Description: Vector Backbone:Lentivirus; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_176070 Copy
Species: Homo sapiens
Genetic Insert: WWE
Vector Backbone Description: Vector Backbone:Lentivirus; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_176072 Copy
Species: Homo sapiens
Genetic Insert: LivePAR (Y107A)
Vector Backbone Description: Vector Backbone:Lentivirus; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_176073 Copy
Species: Homo sapiens
Genetic Insert: Light chain of Fab_8D3_2
Vector Backbone Description: Backbone Marker:addgene; Backbone Size:4879; Vector Backbone:pCAGEN; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_176076 Copy
Species: Homo sapiens
Genetic Insert: His6 tagged heavy chain of Fab_8D3_2
Vector Backbone Description: Backbone Marker:addgene; Backbone Size:4879; Vector Backbone:pCAGEN; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_176075 Copy
Species: Homo sapiens
Genetic Insert: Ubiquitin C
Vector Backbone Description: Backbone Size:4800; Vector Backbone:pRK5-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: The wild-type ubiquitin sequence in this plasmid is: MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGG
Proper citation: RRID:Addgene_17608 Copy
Species: Homo sapiens
Genetic Insert: LRRK2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1 myc/his A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Compared to GenBank reference sequence NP_940980.3, the LRRK2 insert in this plasmid contains a M2397T mutation.
Proper citation: RRID:Addgene_17609 Copy
Species: Homo sapiens
Genetic Insert: Paxillin
Vector Backbone Description: Vector Backbone:pLV; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_176107 Copy
Species: Homo sapiens
Genetic Insert: PBM
Vector Backbone Description: Vector Backbone:Lentivirus; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_176069 Copy
Species: Homo sapiens
Genetic Insert: TIAM1
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: Please visit https://doi.org/10.1101/2022.10.17.512253 for bioRxiv preprint.
Proper citation: RRID:Addgene_176104 Copy
Species: Homo sapiens
Genetic Insert: ITSN1
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: Please visit https://doi.org/10.1101/2022.10.17.512253 for bioRxiv preprint.
Proper citation: RRID:Addgene_176103 Copy
Species: Homo sapiens
Genetic Insert: FHA
Vector Backbone Description: Vector Backbone:Lentivirus; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_176065 Copy
Species: Homo sapiens
Genetic Insert: Ubiquitin C
Vector Backbone Description: Backbone Size:4800; Vector Backbone:pRK5-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please note that Addgene's sequencing results identified P19S and E24D mutations in the ubiquitin sequence. These mutations were not intentional, but were present in the original source clone from which this plasmid was created (see associated publication for details).
Proper citation: RRID:Addgene_17607 Copy
Species: Homo sapiens
Genetic Insert: TIAM1
Vector Backbone Description: Vector Backbone:pEntr1A; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
References:
Comments: Please note: Plasmid contains N394S, NV416-417KL, I524S, and E631G mutations in the mcherry-FKBP-TIAM1(DHPH) insert. These mutations are not known to affect plasmid function. Please visit https://doi.org/10.1101/2022.10.17.512253 for bioRxiv preprint.
Proper citation: RRID:Addgene_176135 Copy
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