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Species: Homo sapiens
Genetic Insert: OPN-b
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5300; Vector Backbone:pDest490; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Addgene QC results detected a D102G mutation in the insert. This variant was present in the original sample and should not alter the function of the plasmid as described in the associated publication.
Proper citation: RRID:Addgene_17591 Copy
Species: Homo sapiens
Genetic Insert: ZMAT4-Nickase1
Vector Backbone Description: Backbone Marker:addgene ID: 62987; Vector Backbone:pX462; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
References:
Comments: gRNA sequence also includes uncloned PAM
Proper citation: RRID:Addgene_175871 Copy
Species: Homo sapiens
Genetic Insert: Myc
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_176049 Copy
Species: Homo sapiens
Genetic Insert: HEY2-Nickase1
Vector Backbone Description: Backbone Marker:addgene ID: 62987; Vector Backbone:pX462; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
References:
Comments: gRNA sequence also includes uncloned PAM
Proper citation: RRID:Addgene_175873 Copy
Species: Homo sapiens
Genetic Insert: HEY2-Nickase2
Vector Backbone Description: Backbone Marker:addgene ID: 62987; Vector Backbone:pX462; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
References:
Comments: gRNA sequence also includes uncloned PAM
Proper citation: RRID:Addgene_175874 Copy
Species: Homo sapiens
Genetic Insert: interferon-gamma cDNA
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:2665; Vector Backbone:pUC8; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: IFN-gamma cDNA. Young lab stock #D2994.
Proper citation: RRID:Addgene_17600 Copy
Species: Homo sapiens
Genetic Insert: Ubiquitin C
Vector Backbone Description: Backbone Size:4800; Vector Backbone:pRK5-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please note that Addgene's sequencing results identified a V5 deletion in the ubiquitin sequence. This deletion was not intentional, but was present in the original source clone from which this plasmid was created (see associated publication for details).
Proper citation: RRID:Addgene_17602 Copy
Species: Homo sapiens
Genetic Insert: Ubiquitin C
Vector Backbone Description: Backbone Size:4800; Vector Backbone:pRK5-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_17604 Copy
Species: Homo sapiens
Genetic Insert: Ubiquitin C
Vector Backbone Description: Backbone Size:4800; Vector Backbone:pRK5-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_17605 Copy
Species: Homo sapiens
Genetic Insert: X-ray repair cross complementing 1
Vector Backbone Description: Backbone Marker:VectorBuilder; Vector Backbone:Lentivirus; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_176062 Copy
Species: Homo sapiens
Genetic Insert: Ubiquitin C
Vector Backbone Description: Backbone Size:4800; Vector Backbone:pRK5-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_17606 Copy
Species: Homo sapiens
Genetic Insert: DDX41
Vector Backbone Description: Backbone Size:6217; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_175845 Copy
Species: Homo sapiens
Genetic Insert: ERBB2
Vector Backbone Description: Backbone Marker:Addgene; Backbone Size:9377; Vector Backbone:pLX304; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_175846 Copy
Species: Homo sapiens
Genetic Insert: WIPI2B (S395E)
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:6143; Vector Backbone:pHTN; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_176006 Copy
Species: Homo sapiens
Genetic Insert: OPN-NT
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5300; Vector Backbone:pDest490; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_17594 Copy
Species: Homo sapiens
Genetic Insert: IFN gamma promoter with 1st intron enhancer
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4800; Vector Backbone:pGL3-basic; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Young lab stock #D3314.
SalI should give 8.6kb + 0.6kb bands.
Proper citation: RRID:Addgene_17598 Copy
Species: Homo sapiens
Genetic Insert: IFN gamma promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4800; Vector Backbone:pGL3-basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments: Young lab stock #D3299.
SalI should give 8.5kb band
Proper citation: RRID:Addgene_17599 Copy
Species: Homo sapiens
Genetic Insert: ZBED9-Nickase1
Vector Backbone Description: Backbone Marker:addgene ID: 62987; Vector Backbone:pX462; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
References:
Comments: gRNA sequence also includes uncloned PAM
Proper citation: RRID:Addgene_175854 Copy
Species: Homo sapiens
Genetic Insert: CHCHD3-Nickase1
Vector Backbone Description: Backbone Marker:addgene ID: 62987; Vector Backbone:pX462; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
References:
Comments: gRNA sequence also includes uncloned PAM
Proper citation: RRID:Addgene_175857 Copy
Species: Homo sapiens
Genetic Insert: CDX4-Nickase1
Vector Backbone Description: Backbone Marker:addgene ID: 62987; Vector Backbone:pX462; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
References:
Comments: gRNA sequence also includes uncloned PAM
Proper citation: RRID:Addgene_175852 Copy
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