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Species: Mus musculus
Genetic Insert: 2kb genomic DNA sequence from murine CD45.1
Vector Backbone Description: Backbone Marker:Genscript; Backbone Size:2710; Vector Backbone:pUC57; Vector Types:HDR template for gene editing; Bacterial Resistance:Ampicillin
References:
Comments: This HDR template can be used in conjunction with sgRNA CD45.2 (Addgene ID 82672). In the template we introduced a PAM mutation. These plasmids were previously associated with the following preprint: Highly efficient and versatile plasmid-based gene editing in primary T cells bioRxiv. 13 January 2018. doi:10.1101/247544
Proper citation: RRID:Addgene_82662 Copy
Species: Mus musculus
Genetic Insert: 4kb genomic DNA sequence from murine CD45.1
Vector Backbone Description: Backbone Marker:Genscript; Backbone Size:2710; Vector Backbone:pUC57; Vector Types:HDR template for gene editing; Bacterial Resistance:Ampicillin
References:
Comments: This HDR template can be used in conjunction with sgRNA CD45.2 (Addgene ID 82672). In the template we introduced a PAM mutation. These plasmids were previously associated with the following preprint: Highly efficient and versatile plasmid-based gene editing in primary T cells bioRxiv. 13 January 2018. doi:10.1101/247544
Proper citation: RRID:Addgene_82663 Copy
Species: Mus musculus
Genetic Insert: 1kb genomic DNA sequence from murine CD90.1
Vector Backbone Description: Backbone Marker:Genscript; Backbone Size:2710; Vector Backbone:pUC57; Vector Types:HDR template for gene editing; Bacterial Resistance:Ampicillin
References:
Comments: This HDR template can be used in conjunction with sgRNA CD90.2 (Addgene ID 82670 or Addgene ID 82671). In the template we introduced a PAM mutation. These plasmids were previously associated with the following preprint: Highly efficient and versatile plasmid-based gene editing in primary T cells bioRxiv. 13 January 2018. doi:10.1101/247544
Proper citation: RRID:Addgene_82667 Copy
Species: Mus musculus
Genetic Insert: 2kb genomic DNA sequence from murine CD90.1
Vector Backbone Description: Backbone Marker:Genscript; Backbone Size:2710; Vector Backbone:pUC57; Vector Types:HDR template for gene editing; Bacterial Resistance:Ampicillin
References:
Comments: This HDR template can be used in conjunction with sgRNA CD90.2 (Addgene ID 82670 or Addgene ID 82671). In the template we introduced a PAM mutation. These plasmids were previously associated with the following preprint: Highly efficient and versatile plasmid-based gene editing in primary T cells bioRxiv. 13 January 2018. doi:10.1101/247544
Proper citation: RRID:Addgene_82668 Copy
Species: Mus musculus
Genetic Insert: 4kb genomic DNA sequence from murine CD90.1
Vector Backbone Description: Backbone Marker:Genscript; Backbone Size:2710; Vector Backbone:pUC57; Vector Types:HDR template for gene editing; Bacterial Resistance:Ampicillin
References:
Comments: This HDR template can be used in conjunction with sgRNA CD90.2 (Addgene ID 82670 or Addgene ID 82671). In the template we introduced a PAM mutation. These plasmids were previously associated with the following preprint: Highly efficient and versatile plasmid-based gene editing in primary T cells bioRxiv. 13 January 2018. doi:10.1101/247544
Proper citation: RRID:Addgene_82669 Copy
Species: Mus musculus
Genetic Insert: 1kb genomic DNA sequence from murine CD45.1
Vector Backbone Description: Backbone Marker:Genscript; Backbone Size:2710; Vector Backbone:pUC57; Vector Types:HDR template for gene editing; Bacterial Resistance:Ampicillin
References:
Comments: This HDR template can be used in conjunction with sgRNA CD45.2 (Addgene ID 82672). In the template we introduced a PAM mutation. These plasmids were previously associated with the following preprint: Highly efficient and versatile plasmid-based gene editing in primary T cells bioRxiv. 13 January 2018. doi:10.1101/247544
Proper citation: RRID:Addgene_82661 Copy
Species: Mus musculus
Genetic Insert: NLS-tdiRFP670-LBR
Vector Backbone Description: Vector Backbone:pPB-LR5; Vector Types:Mammalian Expression, piggyBac vector; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_82620 Copy
Species: Mus musculus
Genetic Insert: 2x DHFR
Vector Backbone Description: Backbone Size:5035; Vector Backbone:pGST-parallele; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_83073 Copy
Species: Mus musculus
Genetic Insert: shId2
Vector Backbone Description: Backbone Marker:Addgene #21915; Backbone Size:10633; Vector Backbone:Tet-pLKO-puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments: The targeting sequence was identified from the TRC ( TRCN0000218289). Knockdown validated by Western blot of transient, ectopic expression of murine Id2 in 293T cells.
Proper citation: RRID:Addgene_83090 Copy
Species: Mus musculus
Genetic Insert: Stat5A
Vector Backbone Description: Backbone Marker:Cell Biolabs; Vector Backbone:pMX-Neo; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_83249 Copy
Species: Mus musculus
Genetic Insert: ANKRD24
Vector Backbone Description: Backbone Marker:Vector Builder ; Backbone Size:3855; Vector Backbone:pRP[Exp]-CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_83190 Copy
Species: Mus musculus
Genetic Insert: DFNB31
Vector Backbone Description: Backbone Marker:Vector Builder ; Backbone Size:3855; Vector Backbone:pRP[Exp]-CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_83196 Copy
Species: Mus musculus
Genetic Insert: PDZD7
Vector Backbone Description: Backbone Marker:Vector Builder ; Backbone Size:3855; Vector Backbone:pRP[Exp]-CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_83198 Copy
Species: Mus musculus
Genetic Insert: FoxO1
Vector Backbone Description: Backbone Marker:Invivogen; Backbone Size:3390; Vector Backbone:pSELECT-puro; Vector Types:Mammalian Expression; Bacterial Resistance:None
References:
Comments: To construct pSELECT-HA-mFOXO1 the coding sequence of HA tag and FOXO1 were amplified from pCMV-HA-FOXO1 by PCR. The PCR product was digested with NheI and SalI restriction enzymes and inserted into the NheI and SalI sites of pSELECT-puro.
The insert was sequenced and compared to mouse FOXO1 (NM_019739.3). Sequence identity was confirmed except for a conservative A->G mutation at base 474, a non-conservative A->G mutation at base 1121 and a non-conservative mutation T->C mutation at base 2321 of NM_019739.3. These mutations were confirmed to exist in the original pCMV-HA-FOXO1 plasmid. The non-conservative mutations result in a K->R substitution at amino acid 219 and a L->P substitution at amino acid 619 of mFOXO1 (NP_062713.2).
Mutations at bases 1121 and 2321 were reversed to wild type by site-directed mutagenesis and the corrections were confirmed by sequencing
Proper citation: RRID:Addgene_83308 Copy
Species: Mus musculus
Genetic Insert: Stat5b
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pAD/CMV/V5-DEST; Vector Types:Adenoviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_83262 Copy
Species: Mus musculus
Genetic Insert: Stat5A
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pAD/CMV/V5-DEST; Vector Types:Adenoviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_83253 Copy
Species: Mus musculus
Genetic Insert: Stat5a
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pAD/CMV/V5-DEST; Vector Types:Adenoviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_83252 Copy
Species: Mus musculus
Genetic Insert: TRIOBP
Vector Backbone Description: Backbone Marker:Vector Builder ; Backbone Size:3855; Vector Backbone:pRP[Exp]-CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_83201 Copy
Species: Mus musculus
Genetic Insert: SLC9A3R2
Vector Backbone Description: Backbone Marker:Vector Builder ; Backbone Size:3855; Vector Backbone:pRP[Exp]-CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_83200 Copy
Species: Mus musculus
Genetic Insert: Rab21
Vector Backbone Description: Backbone Size:4681; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_83422 Copy
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