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Species: Homo sapiens
Genetic Insert: protein kinase B beta
Vector Backbone Description: Backbone Size:6600; Vector Backbone:pLNCX; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Comments: There is a silent mutation at nt552 (CGG->CGA) of the depositor's seq. This mutation does not alter the amino acid sequence.
Proper citation: RRID:Addgene_27295 Copy
Species: Danio rerio
Genetic Insert: Zinc finger array targeting fox1 (a2bp1l)
Vector Backbone Description: Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18657511
Comments: This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene fox1 (a2bp1l) (see below). Please note that this plasmid does NOT contain the fox1 (a2bp1l) sequence.
Users must order the complementary plasmid fox1 (a2bp1l)_R (OZ520) [Addgene plasmid 27195] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene.
Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203).
This zinc finger array was tested for binding activity to the sequence 5'-GACGGAGTC-3' in a bacterial two hybrid assay, and resulted in 7.12 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus).
Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication.
Other Articles:
"Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082
"Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934
Proper citation: RRID:Addgene_27194 Copy
Species: Danio rerio
Genetic Insert: Zinc finger array targeting pitrm1
Vector Backbone Description: Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18657511
Comments: This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene pitrm1 (see below). Please note that this plasmid does NOT contain the pitrm1 sequence.
Users must order the complementary plasmid pitrm1_L (OZ515) [Addgene plasmid 27190] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene.
Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203).
This zinc finger array was tested for binding activity to the sequence 5'-GAAGAAGAG-3' in a bacterial two hybrid assay, and resulted in 8.11 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus).
Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication.
Other Articles:
"Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082
"Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934
Proper citation: RRID:Addgene_27191 Copy
Species: Homo sapiens
Genetic Insert: Smad3
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4900; Vector Backbone:pGEX-6P; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19917253
Proper citation: RRID:Addgene_27222 Copy
Species: Homo sapiens
Genetic Insert: TREX1
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20871604
Proper citation: RRID:Addgene_27220 Copy
Species: Homo sapiens
Genetic Insert: low density lipoprotein receptor-related protein 6
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:xenopus injection; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14731402
Comments: Contains full-length human LRP6. Please note that the VSVG-tag was inserted after the LRP6 signal peptide at amino acid 25.
Proper citation: RRID:Addgene_27282 Copy
Species: Mus musculus
Genetic Insert: Pax3
Vector Backbone Description: Backbone Size:5600; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7744814
Proper citation: RRID:Addgene_27319 Copy
Species: Mus musculus
Genetic Insert: Taz
Vector Backbone Description: Backbone Size:5800; Vector Backbone:pEF-BOS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_27318 Copy
Species: Homo sapiens
Genetic Insert: integrin alpha 5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6200; Vector Backbone:pEF1/V5-HisA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11323715
Comments: There is a minor discrepancy between BLAST truncation position and depositor's truncation position. The discrepancies could be the secretion peptide that the depositing lab does not include in their numbering, or the C-terminal tags (that's about 40 aa.)
Proper citation: RRID:Addgene_27313 Copy
Species: Homo sapiens
Genetic Insert: Septin 5
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3791; Vector Backbone:pCMV-Myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18385322
Proper citation: RRID:Addgene_27274 Copy
Species: Homo sapiens
Genetic Insert: Septin 5
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3791; Vector Backbone:pCMV-Myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18385322
Proper citation: RRID:Addgene_27275 Copy
Species: Mus musculus
Genetic Insert: protein kinase B gamma
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6157; Vector Backbone:pcDNA3.1/CT-GFP-Topo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: The GFP is not expressed as there is a stop codon after the HA tag.
mTORC2 reportedly can phosphorylate FSY and FSF sites, so the Y473F mutation should not affect expression or function.
Proper citation: RRID:Addgene_27293 Copy
Species: Homo sapiens
Genetic Insert: integrin alpha V
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6200; Vector Backbone:pEF1/V5-HisA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12230977
Proper citation: RRID:Addgene_27290 Copy
Species: Danio rerio
Genetic Insert: Zinc finger array targeting dpf2
Vector Backbone Description: Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18657511
Comments: This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene dpf2 (see below). Please note that this plasmid does NOT contain the dpf2 sequence.
Users must order the complementary plasmid dpf2_L (OZ531) [Addgene plasmid 27208] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene.
Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203).
This zinc finger array was tested for binding activity to the sequence 5'-GGAGAAACG-3' in a bacterial two hybrid assay, and resulted in 5.71 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus).
Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication.
Other Articles:
"Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082
"Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934
Proper citation: RRID:Addgene_27209 Copy
Species: Danio rerio
Genetic Insert: Zinc finger array targeting dpf2
Vector Backbone Description: Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18657511
Comments: This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene dpf2 (see below). Please note that this plasmid does NOT contain the dpf2 sequence.
Users must order the complementary plasmid dpf2_R (OZ532) [Addgene plasmid 27209] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene.
Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203).
This zinc finger array was tested for binding activity to the sequence 5'-GAAGTAGCA-3' in a bacterial two hybrid assay, and resulted in 8.58 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus).
Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication.
Other Articles:
"Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082
"Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934
Proper citation: RRID:Addgene_27208 Copy
Species: Danio rerio
Genetic Insert: Zinc finger array targeting mdka
Vector Backbone Description: Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18657511
Comments: This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene mdka (see below). Please note that this plasmid does NOT contain the mdka sequence.
Users must order the complementary plasmid mdka (midkine-related growth factor)_L (OZ527) [Addgene plasmid 27204] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene.
Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203).
This zinc finger array was tested for binding activity to the sequence 5'-GCTGGTGGC-3' in a bacterial two hybrid assay, and resulted in 3.75 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus).
Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication.
Other Articles:
"Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082
"Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934
Proper citation: RRID:Addgene_27205 Copy
Genetic Insert: FokI endonuclease
Vector Backbone Description: Backbone Size:5727; Vector Backbone:pMLM802; Vector Types:Mammalian Expression, zebrafish expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17603475
Comments: For expression of a "-" heterodimeric FokI domain. (Miller et al., Nat Biotech 2007) in mammalian cells or zebrafish for ZFN targets with a 7bp spacer (Handel et al 2008, Mol Ther). To clone a zinc finger array into this plasmid, perform the following steps: (1) amplify the zinc finger array coding sequence from a B2H expression vector by PCR using primers OMM429 (5’-GATGACAAATCTAGACCCGGGGAGCG-3’) and OMM430 (5’-CTGCGGCCGCACCGGGTCCTGTGTGGGTTTTTAGGTG-3’), (2) digest the resulting PCR fragment with XbaI and NotI, and (3) clone the digested fragment into MLM802 vector backbone that has been digested with XbaI and NotI. The resulting plasmid will encode a ZFN that can be paired with a ZFN cloned into expression vector MLM800 to target a ZFN target site with a 7 bp spacer (Handel et al. 2008, Mol Ther.).
The difference between pMLM800/802 and pMLM290/292 is the length of the “spacer” sequence in the full ZFN target site. If the spacer is 7 bp, scientists should use pMLM800/802. If the spacer is 5 or 6 bps, scientists should use pMLM290/292.
Proper citation: RRID:Addgene_27203 Copy
Species: Danio rerio
Genetic Insert: Zinc finger array targeting mdka
Vector Backbone Description: Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18657511
Comments: This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene mdka (see below). Please note that this plasmid does NOT contain the mdka sequence.
Users must order the complementary plasmid mdka (midkine-related growth factor)_R (OZ528) [Addgene plasmid 27205] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene.
Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203).
This zinc finger array was tested for binding activity to the sequence 5'-GAGGAGGGT-3' in a bacterial two hybrid assay, and resulted in 7.20 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus).
Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication.
Other Articles:
"Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082
"Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934
Proper citation: RRID:Addgene_27204 Copy
Species: Danio rerio
Genetic Insert: ubiquitin:EGFP reporter
Vector Backbone Description: Backbone Size:4179; Vector Backbone:pDestTol2A2; Vector Types:Tol2 transposon; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21138979
Comments: There are a few nucleotide discrepancies between author sequence and Addgene quality control sequence. According to the depositing lab, these do not affect plasmid function.
Proper citation: RRID:Addgene_27323 Copy
Species: Danio rerio
Genetic Insert: zebrafish ubiquitin promoter
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2692; Vector Backbone:pENTR5' TOPO; Vector Types:Multisite Gateway 5' entry vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21138979
Proper citation: RRID:Addgene_27320 Copy
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