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Genetic Insert: sacB
Vector Backbone Description: Vector Backbone:pDAI-SceI; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:25172289
Comments: Addgene NGS found K43E and T318P within the SacB translation compared to the best match NCBI reference sequence (ACF93714.1). The depositing laboratory confirms that these mutations should not adversely affect plasmid function
Proper citation: RRID:Addgene_113635 Copy
Vector Backbone Description: Backbone Size:6775; Vector Backbone:pMLBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Trimethoprim
Defining Citation: PMID:15925406
Proper citation: RRID:Addgene_113634 Copy
Genetic Insert: LbCas12a
Vector Backbone Description: Vector Backbone:pMBP; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29449511
Proper citation: RRID:Addgene_113518 Copy
Species: Mus musculus
Genetic Insert: I-Ab beta Derp1-117
Vector Backbone Description: Backbone Size:3818; Vector Backbone:pRMHa-3; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29956152
Comments: This construct, when co-expressed with the corresponding alpha chain construct in Drosophila S2 cells and induced with copper sulfate, results in secreted expression of soluble peptide:MHCII complexes. The antigenic peptide sequence (residues 117-127 of Der p 1 allergen) is encoded as a fusion to the N-terminus of I-Ab beta, connected via a Gly-Ser linker. This fusion is encoded just downstream of the signal peptide, which should be cleaved during post-translational processing. Alternative antigenic peptides can be cloned in place of Der p 1 117-127 by exploiting the XmaI and SpeI restriction sites flanking this sequence.
Proper citation: RRID:Addgene_113639 Copy
Genetic Insert: LbCas12a
Vector Backbone Description: Vector Backbone:pMBP; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29449511
Proper citation: RRID:Addgene_113517 Copy
Species: Mus musculus
Genetic Insert: I-Ab alpha BirA
Vector Backbone Description: Backbone Size:3808; Vector Backbone:pRMHa-3; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29956152
Comments: This construct, when co-expressed with the corresponding beta chain construct in Drosophila S2 cells and induced with copper sulfate, results in secreted expression of soluble peptide:MHCII complexes.
Proper citation: RRID:Addgene_113638 Copy
Genetic Insert: TBG promoter, Top tumor suppressor gRNAs
Vector Backbone Description: Vector Backbone:TBG-Vector (Addgene #109396); Vector Types:Mammalian Expression, Lentiviral, AAV, CRISPR, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29503867
Proper citation: RRID:Addgene_113585 Copy
Species: Synthetic
Genetic Insert: EJ7-GFP variant of EGFP
Vector Backbone Description: Vector Backbone:pCMV6-AC; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29950655
Proper citation: RRID:Addgene_113617 Copy
Species: Synthetic
Genetic Insert: NIR-GECO1
Vector Backbone Description: Backbone Size:6160; Vector Backbone:pcDNA; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30664778
Proper citation: RRID:Addgene_113680 Copy
Genetic Insert: N/A
Vector Backbone Description: Backbone Marker:Agilent Technologies; Vector Backbone:AAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30483052
Proper citation: RRID:Addgene_113684 Copy
Genetic Insert: SaCas9
Vector Backbone Description: Backbone Marker:Agilent Technologies; Vector Backbone:AAV; Vector Types:AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30483052
Comments: P2A-HAFLAGHA-KASH was cloned from a custom gBlock.
Proper citation: RRID:Addgene_113688 Copy
Genetic Insert: SaCas9
Vector Backbone Description: Backbone Marker:Agilent Technologies; Vector Backbone:AAV; Vector Types:AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30483052
Comments: P2A-HAFLAGHA-KASH was cloned from a custom gBlock.
Proper citation: RRID:Addgene_113687 Copy
Species: Synthetic
Genetic Insert: de-catalyzed SaCas9
Vector Backbone Description: Backbone Marker:Alligent Technologies; Vector Backbone:AAV; Vector Types:AAV, Cre/Lox, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30483052
Proper citation: RRID:Addgene_113686 Copy
Species: Synthetic
Genetic Insert: inverted de-catalyzed SaCas9
Vector Backbone Description: Backbone Marker:Alligent Technologies; Backbone Size:6739; Vector Backbone:AAV; Vector Types:AAV, Cre/Lox, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30483052
Proper citation: RRID:Addgene_113685 Copy
Species: Synthetic
Genetic Insert: mTagBFP2
Vector Backbone Description: Backbone Size:7894; Vector Backbone:pLenti6.2; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34568302
Proper citation: RRID:Addgene_113725 Copy
Species: Synthetic
Genetic Insert: TagBFP
Vector Backbone Description: Backbone Size:7895; Vector Backbone:pLenti6.2; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_113724 Copy
Genetic Insert: SaCas9
Vector Backbone Description: Backbone Marker:Agilent Technologies; Vector Backbone:AAV; Vector Types:AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30483052
Comments: P2A-HAFLAGHA-KASH was cloned from a custom gBlock.
Proper citation: RRID:Addgene_113689 Copy
Species: Synthetic
Genetic Insert: miRFP670
Vector Backbone Description: Backbone Size:7909; Vector Backbone:pLenti6.2; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_113726 Copy
Species: Homo sapiens
Genetic Insert: Rictor
Vector Backbone Description: Backbone Marker:BD Pharmingen; Backbone Size:4754; Vector Backbone:pRK-5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15268862
Comments: The amino acid sequence of this plasmid matches the NCBI published sequence. No functional differences between the plasmid with the point mutations (Addgene #1860) and this plasmid have been observed thus far. In this version, silent point mutations in the N-terminus were created to destroy the NotI site and facilitate cloning. These will not change the amino acid sequence of the protein. The ORF sequence of the new insert has been uploaded and is linked from this datasheet.
Proper citation: RRID:Addgene_11367 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ulp1_R4
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29976752
Proper citation: RRID:Addgene_113672 Copy
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