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Species: Homo sapiens
Genetic Insert: Potassium voltage-gated channel subfamily E member 1
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3000; Vector Backbone:pSP64; Vector Types:Xenopus Oocyte Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_53049 Copy
Species: Homo sapiens
Genetic Insert: Potassium voltage-gated channel subfamily H member 2
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3017; Vector Backbone:pSP64; Vector Types:Xenopus Oocyte Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_53051 Copy
Species: Homo sapiens
Genetic Insert: Potassium voltage-gated channel subfamily E member 1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:10185; Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_53050 Copy
Species: Homo sapiens
Genetic Insert: Potassium voltage-gated channel subfamily H member 2
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3017; Vector Backbone:pSP64; Vector Types:Xenopus Oocyte Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_53057 Copy
Species: Homo sapiens
Genetic Insert: Potassium voltage-gated channel subfamily H member 2
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3017; Vector Backbone:pSP64; Vector Types:Xenopus Oocyte Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_53058 Copy
Species: Homo sapiens
Genetic Insert: Potassium voltage-gated channel subfamily H member 2
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3017; Vector Backbone:pSP64; Vector Types:Xenopus Oocyte Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_53055 Copy
Species: Homo sapiens
Genetic Insert: Potassium voltage-gated channel subfamily H member 2
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3017; Vector Backbone:pSP64; Vector Types:Xenopus Oocyte Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_53054 Copy
Species: Homo sapiens
Genetic Insert: OCT1
Vector Backbone Description: Vector Backbone:pLenti-CMV-BSD; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_53118 Copy
Species: Homo sapiens
Genetic Insert: Gfa2
Vector Backbone Description: Vector Backbone:pUC18; Vector Types:Mammalian Expression, Mouse Targeting; Bacterial Resistance:Ampicillin
References:
Comments: The plasmid is a pUC18 vector containing the human GFAP sequences from -2163 to +47 (the human gfa2 segment), with the initiating ATG mutated to TTG, placed in front of the nuclear targeted E. coli lacZ gene, which in turn is followed by a fragment of the mouse protamine-1 gene. The latter supplies an intron, stabilizing 3' UTR, and a polyadenylation signal. If cloning a cDNA, it is advisable to retain the mP-1 segment. The lacZ gene can be excised by digestion with BamHI, and replaced with your gene of interest. If cloning a genomic sequence that includes an intron and polyadenylation site (this may compromise astrocyte specificity--see Su et al. 2004), the mP-1 region can also be excised, or alternatively, a number of sites flank the gfa2 segment allowing its isolation.
Restriction sites that may be useful are as follows:
EcoRI: flanks the gfa2, lacZ, and mP-1 segments
Bgl II: 5' flank of gfa2 & 3' flank of mP-1
Bam HI: flanks the lacZ gene
Sal I: cuts uniquely between the gfa2 promoter and the LacZ gene
Sph I and Hind III: cut uniquely just 3' of the mP-1 segment
Although the GFAP promoter appears to be the best choice for targeting transgene expression to astrocytes in mice, please be aware that neuronal expression has also occasionally been observed (Su et al. 2004).
Proper citation: RRID:Addgene_53126 Copy
Species: Homo sapiens
Genetic Insert: Gfa2
Vector Backbone Description: Vector Backbone:pUC18; Vector Types:Mammalian Expression, Mouse Targeting; Bacterial Resistance:Ampicillin
References:
Comments: This plasmid is a variant of pGfa2-nLac (Addgene plasmid #53126) constructed by inserting 3 copies of the gfa2 B segment (Besnard et al. 1991) into the Sma I site just upstream of the basal promoter. It has about 75 times higher activity than pGfa2-nLac in transfected cells, but does not work in transgenic mice; possibly the high level of expression it produces is toxic.
Proper citation: RRID:Addgene_53132 Copy
Species: Homo sapiens
Genetic Insert: CD59
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6651; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Article ref 86
Proper citation: RRID:Addgene_53154 Copy
Species: Homo sapiens
Genetic Insert: MEK1
Vector Backbone Description: Backbone Size:5100; Vector Backbone:pWZLblasti; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_53161 Copy
Species: Homo sapiens
Genetic Insert: ERK2
Vector Backbone Description: Backbone Marker:GE Healthcare; Backbone Size:4968; Vector Backbone:pGEX4T3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: The sequence discrepancies found during the QC process have no functional consequence.
Proper citation: RRID:Addgene_53202 Copy
Species: Homo sapiens
Genetic Insert: MEK5
Vector Backbone Description: Backbone Size:5100; Vector Backbone:pWZLblasti; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: The sequence discrepancies found during the QC process have no functional consequence.
Proper citation: RRID:Addgene_53167 Copy
Species: Homo sapiens
Genetic Insert: ERK2
Vector Backbone Description: Backbone Marker:GE Healthcare; Backbone Size:4968; Vector Backbone:pGEX4T3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: The sequence discrepancies found during the QC process have no functional consequence.
Proper citation: RRID:Addgene_53200 Copy
Species: Homo sapiens
Genetic Insert: MEK1
Vector Backbone Description: Backbone Size:5100; Vector Backbone:pWZLblasti; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: The L197P mutation found during the QC process has no functional consequence.
Proper citation: RRID:Addgene_53165 Copy
Species: Homo sapiens
Genetic Insert: MEK1
Vector Backbone Description: Backbone Marker:GE Healthcare; Backbone Size:4984; Vector Backbone:pGEX6P1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_53163 Copy
Species: Homo sapiens
Genetic Insert: MEK1
Vector Backbone Description: Backbone Marker:GE Healthcare; Backbone Size:4984; Vector Backbone:pGEX6P1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_53164 Copy
Species: Homo sapiens
Genetic Insert: TRF2 Delta Basic
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4404; Vector Backbone:pTrcHisB; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please note that the correct TERF2 reference sequence for this plasmid is Q15554.2 (not the more recent Q15554.3 version).
Proper citation: RRID:Addgene_53208 Copy
Species: Homo sapiens
Genetic Insert: TRF1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4404; Vector Backbone:pTrcHisB; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_53209 Copy
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