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Species: Mus musculus
Genetic Insert: Stat3 S727A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pRc/CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Diagnostic Restriction Digest with BamHI (3.2kb, 3.1kb, and 2.0kb).
* E16K mutation found during QC
Proper citation: RRID:Addgene_8708 Copy
Species: Mus musculus
Genetic Insert: Stat3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pRc/CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Diagnostic Restriction Digest with BamHI (3.2kb, 2.8kb, and 2.0kb). Due to a stop codon error made when tagging the Stat3 clone with FLAG (DYKDDDDK) additional residues precede (SR) and follow the tag (GPYSIVSPKC STOP). The ApaI cloning site is GGG CCC, which encodes the G and P residues coming after the FLAG tag. Therefore, if the insert is removed with a NotI and ApaI digestion, it will not have a stop codon included.
* E16K mutation found during QC
Proper citation: RRID:Addgene_8707 Copy
Species: Mus musculus
Genetic Insert: Stat3
Vector Backbone Description: Backbone Marker:Identical to pRc/CMV except at its MCS; Backbone Size:5400; Vector Backbone:pcDNA3.0; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Diagnostic restriction digest with XmaI (1.6kb and 6.1kb).
* E16K mutation found during QC. This variant is found in other published versions of this protein; should function as described in associated publication.
Proper citation: RRID:Addgene_8706 Copy
Species: Mus musculus
Genetic Insert: TRIM30B
Vector Backbone Description: Backbone Size:5427; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_87082 Copy
Species: Mus musculus
Genetic Insert: TRIM12C
Vector Backbone Description: Backbone Size:5427; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_87080 Copy
Species: Mus musculus
Genetic Insert: TRIM30C
Vector Backbone Description: Backbone Size:5427; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_87083 Copy
Species: Mus musculus
Genetic Insert: TRIM12B
Vector Backbone Description: Backbone Size:5427; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_87079 Copy
Species: Mus musculus
Genetic Insert: Setd7
Vector Backbone Description: Vector Backbone:pCS2+ MycTag; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_87131 Copy
Species: Mus musculus
Genetic Insert: TZAP
Vector Backbone Description: Vector Backbone:pCDNA-H1-sgRNA; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_87187 Copy
Species: Mus musculus
Genetic Insert: GLI1
Vector Backbone Description: Vector Backbone:NEB DHFR control plasmid; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: The DNA binding domain of zinc finger protein GLI1 in mouse genome, used for expression in E. coli.
Note: There is a K58R mutation in SUMO in this plasmid, the depositor has confirmed that it does not affect plasmid function.
Proper citation: RRID:Addgene_87225 Copy
Species: Mus musculus
Genetic Insert: MYO10
Vector Backbone Description: Backbone Marker:Thermo Fisher; Backbone Size:5428; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_87255 Copy
Species: Mus musculus
Genetic Insert: MYO10
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_87256 Copy
Species: Mus musculus
Genetic Insert: MYO10
Vector Backbone Description: Backbone Marker:Thermo Scientific; Backbone Size:2900; Vector Backbone:pIZ/V5-His; Vector Types:Insect Expression; Bacterial Resistance:Bleocin (Zeocin)
References:
Comments:
Proper citation: RRID:Addgene_87257 Copy
Species: Mus musculus
Genetic Insert: Cre-dependent mouse codon-optimized Flp recombinase.
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:5601; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_87306 Copy
Species: Mus musculus
Genetic Insert: Hox11 (genomic fragment)
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript KS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: From primer A1.5 (just 3' of homeobox) to SalI site.
For use in whole-mount and regular in situ probe generation.
Proper citation: RRID:Addgene_8748 Copy
Species: Mus musculus
Genetic Insert: Bcl-2 5' genomic
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript KS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_8747 Copy
Species: Mus musculus
Genetic Insert: Bad
Vector Backbone Description: Backbone Size:7200; Vector Backbone:pSFFV-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_8759 Copy
Species: Mus musculus
Genetic Insert: Bad
Vector Backbone Description: Backbone Size:2746; Vector Backbone:pGEM-4Z; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_8758 Copy
Species: Mus musculus
Genetic Insert: Mcl-1 cDNA PCR fragment
Vector Backbone Description: Backbone Size:3948; Vector Backbone:pCR II; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Use as an external probe for genomic Southerns (for in situ use NotI). Amp and Kan resistance markers.
Proper citation: RRID:Addgene_8757 Copy
Species: Mus musculus
Genetic Insert: BAD
Vector Backbone Description: Backbone Size:7200; Vector Backbone:pSFFV-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_8756 Copy
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