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Species: Homo sapiens
Genetic Insert: pri-miR-29b-1~29a
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Point mutations: insertion (1115bp), deletion (A47, T666) (Unnecessary region for miRNA biogenesis)
Proper citation: RRID:Addgene_51376 Copy
Species: Homo sapiens
Genetic Insert: Lin28B
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_51373 Copy
Species: Homo sapiens
Genetic Insert: Lin28A
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: H147A, H169A
Proper citation: RRID:Addgene_51372 Copy
Species: Homo sapiens
Genetic Insert: pri-let-7b
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_51378 Copy
Species: Homo sapiens
Genetic Insert: Kinesin-associated protein 3
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP-C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_51413 Copy
Species: Homo sapiens
Genetic Insert: pri-let-7e
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_51380 Copy
Species: Homo sapiens
Genetic Insert: DGCR8
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_51386 Copy
Species: Homo sapiens
Genetic Insert: DGCR8
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_51385 Copy
Species: Homo sapiens
Genetic Insert: Lin28A
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_51387 Copy
Species: Homo sapiens
Genetic Insert: pri-let-7g
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_51381 Copy
Species: Homo sapiens
Genetic Insert: DGCR8
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Addgene Quality Control sequencing has found some discrepancies in the full plasmid sequence in the V5 tag and linker region of the plasmid. The V5 tag and DGCR8 insert are not effected.
Proper citation: RRID:Addgene_51383 Copy
Species: Homo sapiens
Genetic Insert: NM_001039802.1
Vector Backbone Description: Backbone Marker:Promega (modified to have 3' multi cloning sites); Vector Backbone:Cdc42 3'UTR; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
References:
Comments: miR-29 seed mutant (position: 578-584, 997-1003, TGCT->GTAG)
Proper citation: RRID:Addgene_51390 Copy
Species: Homo sapiens
Genetic Insert: p85alpha 3'UTR
Vector Backbone Description: Backbone Marker:Promega (modified to have 3' multi cloning sites); Vector Backbone:pGL3-UC; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
References:
Comments: miR-29 seed mutant (TGCT->GTAG)
Proper citation: RRID:Addgene_51393 Copy
Species: Homo sapiens
Genetic Insert: 4I79
Vector Backbone Description: Vector Backbone:pFBOH-LIC; Vector Types:baculovirus expression; Bacterial Resistance:Ampicillin
References:
Comments: http://www.thesgc.org/structures/4I79/
Proper citation: RRID:Addgene_51319 Copy
Species: Homo sapiens
Genetic Insert: 4H4H
Vector Backbone Description: Vector Backbone:pET28a-LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: http://www.thesgc.org/structures/4H4H/
http://www.thesgc.org/structures/2RFI/
http://www.rcsb.org/pdb/explore/explore.do?structureId=3CZU
Proper citation: RRID:Addgene_51314 Copy
Species: Homo sapiens
Genetic Insert: 2Q3F
Vector Backbone Description: Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: http://www.thesgc.org/structures/2Q3F/
Proper citation: RRID:Addgene_51356 Copy
Species: Homo sapiens
Genetic Insert: Dicer
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_51366 Copy
Species: Homo sapiens
Genetic Insert: Dicer
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_51365 Copy
Species: Homo sapiens
Genetic Insert: Ska1 IMAGE 3909951
Vector Backbone Description: Backbone Marker:Addgene Plasmid # 44432; Backbone Size:5977; Vector Backbone:pIC242; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_51403 Copy
Species: Homo sapiens
Genetic Insert: PH-PLCD1 (aa 1–170)
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: The S34T mutation found during the QC process has no functional consequence.
Proper citation: RRID:Addgene_51408 Copy
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