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Species: Homo sapiens
Genetic Insert: SDC1
Vector Backbone Description: Backbone Marker:NRC Biotechnology Research Institute; Backbone Size:4401; Vector Backbone:pTT5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_52370 Copy
Species: Homo sapiens
Genetic Insert: SDC1
Vector Backbone Description: Backbone Marker:NRC Biotechnology Research Institute; Backbone Size:4401; Vector Backbone:pTT5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Note that the last 3 amino acids of hSDC1, ‘FYA’, in the cytoplasmic region is an important PDZ domain binding site. This was not an issue in the associated JBC article, but please be aware of the difference. The truncation of hSDC1 also removed the native stop codon and a portion of the beta-globin polyA signal.
Proper citation: RRID:Addgene_52378 Copy
Species: Homo sapiens
Genetic Insert: ERAS
Vector Backbone Description: Vector Backbone:FUW-TetO- lox; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments: Please note that there are several mismatches in between Addgene's quality control and the depositor's sequence. The mismatches do NOT affect plasmid function.
Proper citation: RRID:Addgene_52417 Copy
Species: Homo sapiens
Genetic Insert: Proximal element of hOCT4 promoter
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL3 promoter; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments: Please note that there are several mismatches in between Addgene's quality control and the depositor's sequence. The mismatches do NOT affect plasmid function.
Proper citation: RRID:Addgene_52415 Copy
Species: Homo sapiens
Genetic Insert: FOXO3a
Vector Backbone Description: Vector Backbone:pECE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Generated from Addgene plasmid #1787, HA-FOXO3a WT
Proper citation: RRID:Addgene_52426 Copy
Species: Homo sapiens
Genetic Insert: Phosphomevalonate kinase
Vector Backbone Description: Backbone Marker:Novagen ; Backbone Size:5708; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_52430 Copy
Species: Homo sapiens
Genetic Insert: mevalonate diphosphate decarboxylase
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3666; Vector Backbone:pET23d(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: The 5’ primer (5’-TTCCCCATGGCCTCGGAGAAGC-3’) encoded an NcoI site harboring the ATG start codon; the 3’ primer (5’-GAGGCTCGAGGGCAGCTGGCTTCGGCAGGC-3’) encoded a XhoI site.
Proper citation: RRID:Addgene_52431 Copy
Species: Homo sapiens
Genetic Insert: GP1BBm
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6618; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Article ref 76
Proper citation: RRID:Addgene_52314 Copy
Species: Homo sapiens
Genetic Insert: SDC1
Vector Backbone Description: Backbone Marker:NRC Biotechnology Research Institute; Backbone Size:4401; Vector Backbone:pTT5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_52326 Copy
Species: Homo sapiens
Genetic Insert: C20
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6749; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Article ref 103
Proper citation: RRID:Addgene_52327 Copy
Species: Homo sapiens
Genetic Insert: SGCD
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6749; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Article ref 111
Proper citation: RRID:Addgene_52333 Copy
Species: Homo sapiens
Genetic Insert: PR594
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6749; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Article ref 110
Proper citation: RRID:Addgene_52332 Copy
Species: Homo sapiens
Genetic Insert: LRRC59
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6749; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Article ref 107
Proper citation: RRID:Addgene_52330 Copy
Species: Homo sapiens
Genetic Insert: TFRC
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6749; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Article ref 116
Proper citation: RRID:Addgene_52338 Copy
Species: Homo sapiens
Genetic Insert: TTMP
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6749; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Article ref 113
Proper citation: RRID:Addgene_52335 Copy
Species: Homo sapiens
Genetic Insert: HPV52 L1
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6295; Vector Backbone:pVITRO1-neo-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_52584 Copy
Species: Homo sapiens
Genetic Insert: human transforming growth factor-beta3
Vector Backbone Description: Backbone Size:10527; Vector Backbone:pLV-tTRKRAB-red; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_52580 Copy
Species: Homo sapiens
Genetic Insert: RIF1
Vector Backbone Description: Backbone Size:7510; Vector Backbone:pDEST pcDNA5/FRT/TO-eGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: The Rif1 insert contains G836S, V1362M, N2021Y, L2392V variants compared to the NCBI reference for isoform 2 [NM_001177663.1] but matches the NCBI protein sequence AAV51401.1.
Proper citation: RRID:Addgene_52506 Copy
Species: Homo sapiens
Genetic Insert: 53BP1
Vector Backbone Description: Backbone Size:5198; Vector Backbone:pcDNA5 FRT/T0-Flag; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_52507 Copy
Species: Homo sapiens
Genetic Insert: BRCA1
Vector Backbone Description: Backbone Size:6856; Vector Backbone:pDEST 3' Triple Flag pcDNA5 FRT TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_52504 Copy
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