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Species: Homo sapiens
Genetic Insert: SHANK-associated RH domain interactor
Vector Backbone Description: Backbone Marker:Agilent (Stratagene); Backbone Size:5171; Vector Backbone:pCMV-3Tag-8; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_50014 Copy
Species: Homo sapiens
Genetic Insert: TRBP
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: Fragment encoding a full length human TRBP was amplified using a cDNA mixture of total RNA extracted from human HeLa cells by PCR with primers containing restriction enzyme sites of HindIII and NotI. The amplified fragment was digested with the same enzyme sites and cloned into pcDNA3.1-myc/His A vector (Invitrogen). C-terminal myc-tagged TRBP fragment was amplified from pcDNA3.1-TRBP using primer with restriction enzyme sites NheI and HindIII. The amplified fragments were digested with same enzyme sites and cloned into pET-28a vector (Novagen).
Proper citation: RRID:Addgene_50351 Copy
Species: Homo sapiens
Genetic Insert: eIF2C1
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:Unknown (a derivative of pVP22/Myc-His); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Addgene sequencing has found an I638L mutation.
Proper citation: RRID:Addgene_50362 Copy
Species: Homo sapiens
Genetic Insert: grass pollen allergen Phl p 7 specific human epsilon heavy chain expression cassette
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
References:
Comments:
Proper citation: RRID:Addgene_50365 Copy
Species: Homo sapiens
Genetic Insert: eIF2C1
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:Unknown (a derivative of pVP22/Myc-His); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Addegene sequencing identified a D45G mutation.
Proper citation: RRID:Addgene_50361 Copy
Species: Homo sapiens
Genetic Insert: eIF2C1
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:Unknown (a derivative of pVP22/Myc-His); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_50360 Copy
Species: Homo sapiens
Genetic Insert: grass pollen allergen Phl p 7 specific human Gamma 1 heavy chain expression cassette
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
References:
Comments:
Proper citation: RRID:Addgene_50366 Copy
Species: Homo sapiens
Genetic Insert: grass pollen allergen Phl p 7 specific human Gamma 4 heavy chain expression cassette
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
References:
Comments:
Proper citation: RRID:Addgene_50369 Copy
Species: Homo sapiens
Genetic Insert: grass pollen allergen Phl p 7 specific human Gamma 3 heavy chain expression cassette
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
References:
Comments:
Proper citation: RRID:Addgene_50368 Copy
Species: Homo sapiens
Genetic Insert: PGAP3 post-GPI attachment to proteins 3
Vector Backbone Description: Vector Backbone:pME-hygro(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_50373 Copy
Species: Homo sapiens
Genetic Insert: MICU2
Vector Backbone Description: Backbone Size:9379; Vector Backbone:PLEX 983; Vector Types:Mammalian Expression, LENTIVIRUS; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_50372 Copy
Species: Homo sapiens
Genetic Insert: eGFP with I-Sce I TS
Vector Backbone Description: Vector Backbone:pCVL.Active/Repressed TLR (Sce); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments: Note that the only difference between this lasmid and Plasmid #45575 is that there is a Puromycin selection marker
Proper citation: RRID:Addgene_50417 Copy
Species: Homo sapiens
Genetic Insert: EPO
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:9387; Vector Backbone:pLenti6.3/V5-DEST gateway vector; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments: Human erythropoietin (hEPO) ORF with stop codon (582 bp; Ultimate ORF clone number IOH7334) from GeneCopoeia ORF cDNA clones in pDONR vector (GeneCopoeia; GC-A1011).
Because the stop codon is inserted before V5 epitope, the V5 tag on the pLenti6.3/V5-DEST vector won’t be transcribed.
Proper citation: RRID:Addgene_50436 Copy
Species: Homo sapiens
Genetic Insert: MKK3b
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_50450 Copy
Species: Homo sapiens
Genetic Insert: MKK3b
Vector Backbone Description: Backbone Marker:David Baltimore (Addgene Plasmid # 22227); Backbone Size:6034; Vector Backbone:pEBG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_50452 Copy
Species: Homo sapiens
Genetic Insert: hM4D(Gi)-IRES-mCitrine
Vector Backbone Description: Backbone Size:4818; Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
References:
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community.
Proper citation: RRID:Addgene_50455 Copy
Species: Homo sapiens
Genetic Insert: hM3D(Gq)-IRES-mCitrine
Vector Backbone Description: Backbone Size:4818; Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
References:
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community.
Proper citation: RRID:Addgene_50454 Copy
Species: Homo sapiens
Genetic Insert: hM4D(Gi)-IRES-mCitrine
Vector Backbone Description: Backbone Size:4818; Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
References:
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community.
Proper citation: RRID:Addgene_50464 Copy
Species: Homo sapiens
Genetic Insert: hM3D(Gq)-IRES-mCitrine
Vector Backbone Description: Backbone Size:4818; Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
References:
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community.
Proper citation: RRID:Addgene_50463 Copy
Species: Homo sapiens
Genetic Insert: hM3D(Gq)-mCherry
Vector Backbone Description: Backbone Size:4818; Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
References:
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community.
Proper citation: RRID:Addgene_50460 Copy
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