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Species: Homo sapiens
Genetic Insert: MePCE
Vector Backbone Description: Backbone Marker:GE Healthcare Life Sciences; Backbone Size:4972; Vector Backbone:pGEX-5X-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29425494
Proper citation: RRID:Addgene_113543 Copy
Species: Homo sapiens
Genetic Insert: MePCE
Vector Backbone Description: Backbone Marker:GE Healthcare Life Sciences; Backbone Size:4972; Vector Backbone:pGEX-5X-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29425494
Proper citation: RRID:Addgene_113542 Copy
Species: Homo sapiens
Genetic Insert: MePCE
Vector Backbone Description: Backbone Marker:Thermo Fisher; Backbone Size:5344; Vector Backbone:pcDNA5/FRT/TO/intron; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29425494
Proper citation: RRID:Addgene_113549 Copy
Species: Other
Genetic Insert: Flag
Vector Backbone Description: Backbone Marker:Thermo Fisher; Backbone Size:5344; Vector Backbone:pcDNA5/FRT/TO/intron; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29425494
Proper citation: RRID:Addgene_113548 Copy
Species: Homo sapiens
Genetic Insert: shMePCE
Vector Backbone Description: Backbone Marker:OligoEngine; Backbone Size:6349; Vector Backbone:pSUPER.retro.puro; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29425494
Proper citation: RRID:Addgene_113536 Copy
Species: Synthetic
Genetic Insert: shNC (scrambled control shRNA)
Vector Backbone Description: Backbone Marker:OligoEngine; Backbone Size:6349; Vector Backbone:pSUPER.retro.puro; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29425494
Proper citation: RRID:Addgene_113535 Copy
Genetic Insert: BirA Biotin Ligase
Vector Backbone Description: Backbone Size:3830; Vector Backbone:pRMHa-3; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29956152
Comments: This gene encodes a biotin ligase that mediates in vivo biotinylation of BirA signal sequences on recombinant proteins co-expressed in the same cells.
Proper citation: RRID:Addgene_113640 Copy
Species: Synthetic
Genetic Insert: mRFP1-linker-sfGFP
Vector Backbone Description: Vector Backbone:pQE-80L. ColE1; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27648665
Comments: https://benchling.com/s/IF2YcdLA
Proper citation: RRID:Addgene_113642 Copy
Species: Other
Genetic Insert: ndmC
Vector Backbone Description: Vector Backbone:pSB1C3; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31540989
Comments: https://benchling.com/s/COGhnD32 . Please visit https://www.biorxiv.org/content/early/2018/09/16/419283 for bioRxiv preprint.
Proper citation: RRID:Addgene_113648 Copy
Species: Other
Genetic Insert: ndmB
Vector Backbone Description: Vector Backbone:pSB1C3; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31540989
Comments: https://benchling.com/s/2C3Oh1ew . Please visit https://www.biorxiv.org/content/early/2018/09/16/419283 for bioRxiv preprint.
Proper citation: RRID:Addgene_113647 Copy
Species: Other
Genetic Insert: ndmA
Vector Backbone Description: Vector Backbone:pSB1C3; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31540989
Comments: https://benchling.com/s/4vRm4arA . Please visit https://www.biorxiv.org/content/early/2018/09/16/419283 for bioRxiv preprint.
Proper citation: RRID:Addgene_113646 Copy
Species: Synthetic
Genetic Insert: IodoYRS and tRNA gene
Vector Backbone Description: Vector Backbone:pACYC184. p15A; Vector Types:Synthetic Biology; Bacterial Resistance:Gentamicin
Defining Citation: PMID:27648665
Comments: https://benchling.com/s/vJhpn35J
Proper citation: RRID:Addgene_113645 Copy
Genetic Insert: sgRNA cloning site
Vector Backbone Description: Backbone Marker:Broad Institute; Backbone Size:5180; Vector Backbone:pLX_TRC931; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30971695
Proper citation: RRID:Addgene_113591 Copy
Genetic Insert: Hybrid Promoter : Plas_lac_tet_bD
Vector Backbone Description: Backbone Size:3886; Vector Backbone:pmb1, +ROP; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29302024
Proper citation: RRID:Addgene_113595 Copy
Genetic Insert: Hybrid Promoter : Plux_lac_tet_bD
Vector Backbone Description: Backbone Size:3886; Vector Backbone:pmb1, +ROP; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29302024
Comments: Note: Plasmid contains a tet operator instead of a lac operator between rop and myc. This change is not known to affect plasmid function.
Proper citation: RRID:Addgene_113594 Copy
Genetic Insert: Hybrid Promoter : Prhl_lac_tet_bD
Vector Backbone Description: Backbone Size:3886; Vector Backbone:pmb1, +ROP; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29302024
Comments: Note: Plasmid contains a tet operator instead of a lac operator between rop and myc. This change is not known to affect plasmid function.
Proper citation: RRID:Addgene_113593 Copy
Species: E. coli
Genetic Insert: Chloramphenicol resistance gene
Vector Backbone Description: Vector Backbone:pCas; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29884781
Proper citation: RRID:Addgene_113633 Copy
Species: Homo sapiens
Genetic Insert: p97
Vector Backbone Description: Vector Backbone:pFL; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30344098
Proper citation: RRID:Addgene_113510 Copy
Species: Other
Genetic Insert: mKaxxte2.5
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30804405
Comments: One clones a fragment of DNA sequence at the MCS, then uses a targeted nuclease, such as Cas9 to trigger a double-strand break. This is then repaired by homologous recombination resulting in restoration of an intact mKate2.5 fluorescent protein (cloned from Addgene #54828). It can be used to test Cas9 gRNA efficiency, as it was in the paper, or HR-capacity in different cells.
Proper citation: RRID:Addgene_113630 Copy
Species: Homo sapiens
Genetic Insert: I3
Vector Backbone Description: Vector Backbone:pFL; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30344098
Proper citation: RRID:Addgene_113515 Copy
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