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Species: Homo sapiens
Genetic Insert: Rab32
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:EGFP C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_49611 Copy
Species: Homo sapiens
Genetic Insert: Rab31
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:EGFP C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_49610 Copy
Species: Homo sapiens
Genetic Insert: Rab35
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:EGFP C2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_49614 Copy
Species: Homo sapiens
Genetic Insert: GPBP
Vector Backbone Description: Backbone Marker:c; Backbone Size:4700; Vector Backbone:EGFP C2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: PH binding domain of GPBP recognizes PI4P
Proper citation: RRID:Addgene_49572 Copy
Species: Homo sapiens
Genetic Insert: OSBP-PH (AA 91-179)
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:EGFP C2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_49571 Copy
Species: Homo sapiens
Genetic Insert: Tet methylcytosine dioxygenase 1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6200; Vector Backbone:pEF1a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please note that compared to GenBank reference sequence NM_030625.2, the TET1 insert in this plasmid contains a SNP at amino acid 1123. The resulting I1223M amino acid change does not affect TET1 expression or catalytic activity.
Proper citation: RRID:Addgene_49792 Copy
Species: Homo sapiens
Genetic Insert: YOD1
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_49785 Copy
Species: Homo sapiens
Genetic Insert: YOD1
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_49784 Copy
Species: Homo sapiens
Genetic Insert: steroid receptor RNA activator protein aa 13-87
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:7375; Vector Backbone:pTWIN1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_49816 Copy
Species: Homo sapiens
Genetic Insert: steroid receptor RNA activator protein
Vector Backbone Description: Backbone Marker:none; Backbone Size:6518; Vector Backbone:pHMG6; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_49814 Copy
Species: Homo sapiens
Genetic Insert: Rab30
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:7300; Vector Backbone:GBK-T7; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_49849 Copy
Species: Homo sapiens
Genetic Insert: Rab14
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:7300; Vector Backbone:GBK-T7; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_49844 Copy
Species: Homo sapiens
Genetic Insert: 3x Flag Tet1CD HB-6
Vector Backbone Description: Backbone Marker:Joung lab (Addgene plasmid #49237); Vector Backbone:pJFA334E9; Vector Types:TALEN; Bacterial Resistance:Ampicillin
References:
Comments: TALE target site is: TGCTACTAAAAACATCCTCC.
See Supplementary Figure 13 of associated publication for amino acid sequences of backbone and TALE repeat array.
Proper citation: RRID:Addgene_49964 Copy
Species: Homo sapiens
Genetic Insert: 3x Flag Tet1CDmut HB-5
Vector Backbone Description: Backbone Marker:Joung lab (Addgene plasmid #49239); Vector Backbone:pJFA274; Vector Types:TALEN; Bacterial Resistance:Ampicillin
References:
Comments: TALE target site is: TATGCTTACCAAGCTGTGAT. Note that due to the TET1 mutations, this plasmid neither demethylated the proximal CpGs nor activated HBB gene expression.
See Supplementary Figure 13 of associated publication for amino acid sequences of backbone and TALE repeat array.
Proper citation: RRID:Addgene_49962 Copy
Species: Homo sapiens
Genetic Insert: Tet1CD HB-10
Vector Backbone Description: Backbone Marker:Joung lab (Addgene plasmid #49238); Vector Backbone:pJFA345D4; Vector Types:TALEN; Bacterial Resistance:Ampicillin
References:
Comments: TALE target site is: TGATACCAACCTGCCCAGGG.
See Supplementary Figure 13 of associated publication for amino acid sequences of backbone and TALE repeat array.
Proper citation: RRID:Addgene_49969 Copy
Species: Homo sapiens
Genetic Insert: Rab14
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:7300; Vector Backbone:GBK-T7; Vector Types:; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_49847 Copy
Species: Homo sapiens
Genetic Insert: Tet1CD HB-9
Vector Backbone Description: Backbone Marker:Joung lab (Addgene plasmid #49239); Vector Backbone:pJFA274; Vector Types:TALEN; Bacterial Resistance:Ampicillin
References:
Comments: TALE target site is: TACCAACCTGCCCAGGGCCT.
See Supplementary Figure 13 of associated publication for amino acid sequences of backbone and TALE repeat array.
Proper citation: RRID:Addgene_49968 Copy
Species: Homo sapiens
Genetic Insert: Rab6B
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:7300; Vector Backbone:GBK-T7; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_49839 Copy
Species: Homo sapiens
Genetic Insert: 3x Flag Tet1CDmut HB-4
Vector Backbone Description: Backbone Marker:Joung lab (Addgene plasmid #49238); Vector Backbone:pJFA345D4; Vector Types:TALEN; Bacterial Resistance:Ampicillin
References:
Comments: TALE target site is: TCTTCAATATGCTTACCAAG. Note that due to the TET1 mutations, this plasmid neither demethylated the proximal CpGs nor activated HBB gene expression.
See Supplementary Figure 13 of associated publication for amino acid sequences of backbone and TALE repeat array.
Proper citation: RRID:Addgene_49959 Copy
Species: Homo sapiens
Genetic Insert: Tet1CD HB-1
Vector Backbone Description: Backbone Marker:Joung lab (Addgene plasmid #49236); Vector Backbone:pJFA344C7; Vector Types:TALEN; Bacterial Resistance:Ampicillin
References:
Comments: TALE target site is: TCAACTTCTTATTTGTGTAA.
See Supplementary Figure 13 of associated publication for amino acid sequences of backbone and TALE repeat array.
Proper citation: RRID:Addgene_49954 Copy
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