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Species: Homo sapiens
Genetic Insert: EWSR1 G511A
Vector Backbone Description: Backbone Marker:Iain Fraser(Addgene plasmid #25735); Backbone Size:13286; Vector Backbone:pSLIK-neo; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_50734 Copy
Species: Homo sapiens
Genetic Insert: EWSR1
Vector Backbone Description: Backbone Marker:Iain Fraser(Addgene plasmid #25735); Backbone Size:13286; Vector Backbone:pSLIK-neo; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_50733 Copy
Species: Homo sapiens
Genetic Insert: CrkII
Vector Backbone Description: Vector Backbone:pGZ21dxZ; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_50730 Copy
Species: Homo sapiens
Genetic Insert: CrkII Y221F
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCDNA 3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_50732 Copy
Species: Homo sapiens
Genetic Insert: CrkII
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCDNA 3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_50731 Copy
Species: Homo sapiens
Genetic Insert: Cdc42BPB
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_50759 Copy
Species: Homo sapiens
Genetic Insert: c-Myc -dN2
Vector Backbone Description: Backbone Marker:Yamanaka Lab, Addgene plasmid #18656; Backbone Size:4750; Vector Backbone:pMXs-gw; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_50774 Copy
Species: Homo sapiens
Genetic Insert: c-Myc -dN1
Vector Backbone Description: Backbone Marker:Yamanaka Lab, Addgene plasmid #18656; Backbone Size:4750; Vector Backbone:pMXs-gw; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_50773 Copy
Species: Homo sapiens
Genetic Insert: miR-34A promoter M2
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3-Basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_50828 Copy
Species: Homo sapiens
Genetic Insert: miR-34A promoter M53
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3-Basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_50830 Copy
Species: Homo sapiens
Genetic Insert: Triosephosphate isomerase 1
Vector Backbone Description: Backbone Size:5805; Vector Backbone:p413GPD; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_50719 Copy
Species: Homo sapiens
Genetic Insert: SOS2cat
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments: The discrepancies between the full sequence and Addgene QC sequence do not affect function.
Proper citation: RRID:Addgene_50850 Copy
Species: Homo sapiens
Genetic Insert: cofilin 1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Human cofilin 1 cDNA was PCRed with a 5' primer that had an EcoRI, while the 3' primer had KpnI and EcoRV. EcoRI and KpnI were then used to ligate into pcDNA3.1- (leaving the EcoRV on the 3’ end of the insert).
Forward primer sequence: CGGAATTCACCACCATGGCCTCCGGTGTGGCTGTCTC.
Reverse primer sequence: GGGGTACCGATATCCTCACAAAGGCTTGCCCTCCAGG.
Proper citation: RRID:Addgene_50853 Copy
Species: Homo sapiens
Genetic Insert: cofilin 1 S3A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_50860 Copy
Species: Homo sapiens
Genetic Insert: human NKCC1 (synthetic)
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:5316; Vector Backbone:pcDNA 3.1 neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Plasmid constructed from pcDNA3.1 Flag YFP hNKCC1 WT (NT17). See Addgene plasmid# 49085 (http://www.addgene.org/49085/) for plasmid map of wild-type construct.
Proper citation: RRID:Addgene_50865 Copy
Species: Homo sapiens
Genetic Insert: human NKCC1 (synthetic)
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:5316; Vector Backbone:pcDNA 3.1 neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Plasmid constructed from pcDNA3.1 Flag YFP hNKCC1 WT (NT17). See Addgene plasmid# 49085 (http://www.addgene.org/49085/) for plasmid map of wild-type construct.
Proper citation: RRID:Addgene_50868 Copy
Species: Homo sapiens
Genetic Insert: human NKCC1 (synthetic)
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:5316; Vector Backbone:pcDNA 3.1 neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Plasmid constructed from pcDNA3.1 Flag YFP hNKCC1 WT (NT17). See Addgene plasmid# 49085 (http://www.addgene.org/49085/) for plasmid map of wild-type construct.
Proper citation: RRID:Addgene_50900 Copy
Species: Homo sapiens
Genetic Insert: cofilin 1 S3E
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_50861 Copy
Species: Homo sapiens
Genetic Insert: human NKCC1 (synthetic)
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:5316; Vector Backbone:pcDNA 3.1 neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Plasmid constructed from pcDNA3.1 Flag YFP hNKCC1 WT (NT17). See Addgene plasmid# 49085 (http://www.addgene.org/49085/) for plasmid map of wild-type construct.
Proper citation: RRID:Addgene_50863 Copy
Species: Homo sapiens
Genetic Insert: human NKCC1 (synthetic)
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:5316; Vector Backbone:pcDNA 3.1 neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Plasmid constructed from pcDNA3.1 Flag YFP hNKCC1 WT (NT17). See Addgene plasmid# 49085 (http://www.addgene.org/49085/) for plasmid map of wild-type construct.
Proper citation: RRID:Addgene_50907 Copy
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