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Species: Homo sapiens
Genetic Insert: TNRC6A
Vector Backbone Description: Vector Backbone:custom-made pDEST:gfp vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_21522 Copy
Species: Homo sapiens
Genetic Insert: Cited2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: HA tag added to 5'end of the cited2 cDNA using a blunt-ha-stop-xho oligo. The insert was ligated into the EcoRI/XhoI sites of pcDNA3
Proper citation: RRID:Addgene_21488 Copy
Species: Homo sapiens
Genetic Insert: TNRC6A
Vector Backbone Description: Vector Backbone:custom-made pDEST:gfp vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_21521 Copy
Species: Homo sapiens
Genetic Insert: ALIX
Vector Backbone Description: Backbone Size:4700; Vector Backbone:mCherry; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_21504 Copy
Species: Homo sapiens
Genetic Insert: ALIX
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pGST2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_21502 Copy
Species: Homo sapiens
Genetic Insert: mitogen-activated protein kinase kinase kinase 3
Vector Backbone Description: Backbone Marker:Unknown; Backbone Size:5100; Vector Backbone:pMT2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: lysine mutant activity unknown
Proper citation: RRID:Addgene_21585 Copy
Species: Homo sapiens
Genetic Insert: MKK6
Vector Backbone Description: Backbone Marker:Dr. David Russell, UTSW; Backbone Size:4700; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: M2-FLAG,
Lysine mutant, inactive
Proper citation: RRID:Addgene_21583 Copy
Species: Homo sapiens
Genetic Insert: MKK6
Vector Backbone Description: Backbone Marker:Dr. David Russell, UTSW; Backbone Size:4700; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: M2-FLAG
Proper citation: RRID:Addgene_21582 Copy
Species: Homo sapiens
Genetic Insert: CEP55-EABR (ESCRT and ALIX Binding Region) domain
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pGST2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: PNC to MGS mut in beginning of protein; author says that this is the plasmid that was used in publication.
Proper citation: RRID:Addgene_21501 Copy
Species: Homo sapiens
Genetic Insert: TNF receptor-associated factor 2
Vector Backbone Description: Backbone Marker:Bruce Mayer Lab; Backbone Size:6100; Vector Backbone:pEBG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_21586 Copy
Species: Homo sapiens
Genetic Insert: Hypoxia inducible factor 2 alpha
Vector Backbone Description: Backbone Size:5188; Vector Backbone:pBabe-Hygro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_21676 Copy
Species: Homo sapiens
Genetic Insert: p21 promoter deletion mutant
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:5600; Vector Backbone:pBlueScript II KS(+); Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Chloramphenicol
References:
Comments:
Proper citation: RRID:Addgene_21728 Copy
Species: Homo sapiens
Genetic Insert: IGF-II mRNA-binding protein 1
Vector Backbone Description: Backbone Size:7600; Vector Backbone:pMSCV PIG; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: Short means always shortest 3’UTR due to alternative polyadenylation.
Constructs for Soft-agar assay
Proper citation: RRID:Addgene_21659 Copy
Species: Homo sapiens
Genetic Insert: Cyclin D2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6295; Vector Backbone:pMSCVpuro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: Long means that all functional alternative polyadenylation signals have been deleted.
Mut means that all microRNA binding sites for an indicated microRNA have been mutated.
Constructs for cell cycle
Proper citation: RRID:Addgene_21657 Copy
Species: Homo sapiens
Genetic Insert: Dicer 1
Vector Backbone Description: Backbone Marker:Available from Addgene (#12179); Backbone Size:4085; Vector Backbone:pIS1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments: Long means that all functional alternative polyadenylation signals have been deleted.
Mut means that all microRNA binding sites for an indicated microRNA have been mutated.
Proper citation: RRID:Addgene_21651 Copy
Species: Homo sapiens
Genetic Insert: Cyclin D2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6295; Vector Backbone:pMSCVpuro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: Short means always shortest 3’UTR due to alternative polyadenylation.
Constructs for cell cycle
Proper citation: RRID:Addgene_21655 Copy
Species: Homo sapiens
Genetic Insert: Dicer 1
Vector Backbone Description: Backbone Marker:Available from Addgene (#12179); Backbone Size:4085; Vector Backbone:pIS1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments: Long means that all functional alternative polyadenylation signals have been deleted.
Mut means that all microRNA binding sites for an indicated microRNA have been mutated.
Proper citation: RRID:Addgene_21653 Copy
Species: Homo sapiens
Genetic Insert: E2F-1
Vector Backbone Description: Backbone Marker:Pharmacia; Backbone Size:4959; Vector Backbone:pGex2TK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_21668 Copy
Species: Homo sapiens
Genetic Insert: DEPTOR PDZ domain
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pRK5-FLAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_21701 Copy
Species: Homo sapiens
Genetic Insert: DEPTOR DEP domains
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pRK5-FLAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_21700 Copy
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