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Species: Homo sapiens
Genetic Insert: JMJD3
Vector Backbone Description: Backbone Size:6300; Vector Backbone:pMSCVpuro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_21214 Copy
Species: Homo sapiens
Genetic Insert: PLCdelta
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: This construct encodes a PI(4,5)P2 lipid selective PH domain that can be used as a fluorescent translocation biosensor to monitor changes or local differences in the concentration of plasma membrane PI(4,5)P2 lipids (Stauffer TP, Ahn S, Meyer T; Curr Biol. 1998 8:343-6). It is best used in combination with a second fluorescent protein that uniformly marks the plasma membrane (the last 20 amino acids "CAAX" region of KRas is often used as a PM marker). Relative changes in cell-wide or local plasma membrane PI(4,5)P2 can be determined by using a mask for the PM region and by dividing the two background subtracted PH domain and CAAX images. At high expression levels, this construct interferes with PI(4,5)P2 signaling and alters cortical actin (Raucher D et al Cell. 2000 100:221-8).
Proper citation: RRID:Addgene_21179 Copy
Species: Homo sapiens
Genetic Insert: JMJD3
Vector Backbone Description: Backbone Size:6300; Vector Backbone:pMSCVpuro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_21212 Copy
Species: Homo sapiens
Genetic Insert: E2F transcription factor 1
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5256; Vector Backbone:pGL3-Control; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_21170 Copy
Species: Homo sapiens
Genetic Insert: DUX4
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_21172 Copy
Species: Homo sapiens
Genetic Insert: E2F 3'UTR Mutant
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5256; Vector Backbone:pGL3-Control; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_21171 Copy
Species: Homo sapiens
Genetic Insert: HIF1AN
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_21399 Copy
Species: Homo sapiens
Genetic Insert: TRX1
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3-His; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_21286 Copy
Species: Homo sapiens
Genetic Insert: TRX1
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3-FLAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_21285 Copy
Species: Homo sapiens
Genetic Insert: TRX1
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3-FLAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_21284 Copy
Species: Homo sapiens
Genetic Insert: autosomal dominant polycystic kidney disease type II
Vector Backbone Description: Backbone Size:4352; Vector Backbone:pCS2+MT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: *Note that this construct has a rare SNP (rs1131408) at position 4:88967820 that results in a missense change (G449V). The polymorphism is not known to affect function.
Proper citation: RRID:Addgene_21370 Copy
Species: Homo sapiens
Genetic Insert: VEGFR2 promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
References:
Comments: -225 to +268 region of the promoter.
Proper citation: RRID:Addgene_21306 Copy
Species: Homo sapiens
Genetic Insert: VEGFR2 promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
References:
Comments: -570 to +268 region of the promoter.
Proper citation: RRID:Addgene_21309 Copy
Species: Homo sapiens
Genetic Insert: PLCdelta
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4701; Vector Backbone:C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_21262 Copy
Species: Homo sapiens
Genetic Insert: EGLN1
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3-Basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_21404 Copy
Species: Homo sapiens
Genetic Insert: HIF1AN
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_21403 Copy
Species: Homo sapiens
Genetic Insert: autosomal dominant polycystic kidney disease type I
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:8500; Vector Backbone:pREP10; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please note that Addgene NGS observed a S999P mutation in the PKD1 ORF. The effect of this mutation in unknown.
Proper citation: RRID:Addgene_21368 Copy
Species: Homo sapiens
Genetic Insert: autosomal dominant polycystic kidney disease type I
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4713; Vector Backbone:pCI (modified); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: *Please note: during Addgene's quality control process, the following mutations were found in PKD1: A691P, M792L, S999P, N1056T, T1724A, M1976V. The depositor has noted that the plasmid appears to be functional using several assays but it is not the standard PKD1 sequence in the database (Genbank ID L33243).
Proper citation: RRID:Addgene_21367 Copy
Species: Homo sapiens
Genetic Insert: autosomal dominant polycystic kidney disease type I
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4713; Vector Backbone:pCI (modified); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_21366 Copy
Species: Homo sapiens
Genetic Insert: shMTH1-2
Vector Backbone Description: Backbone Marker:Available at Addgene (#8453); Backbone Size:7000; Vector Backbone:pLKO.1 puro; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
References:
Comments: shRNA #2 directed against MTH1 sequence 5-GAAATTCCACGGGTACTTCAA-3′.
Proper citation: RRID:Addgene_21298 Copy
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