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Species: Homo sapiens
Genetic Insert: Ras
Vector Backbone Description: Backbone Size:11100; Vector Backbone:LZRS-RfA; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_21203 Copy
Species: Homo sapiens
Genetic Insert: miR-20 Control
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5523; Vector Backbone:pGL3-Control; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_21168 Copy
Species: Homo sapiens
Genetic Insert: pBabePuro-bcl 2
Vector Backbone Description: Backbone Size:5035; Vector Backbone:pBabePuro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_21144 Copy
Species: Homo sapiens
Genetic Insert: PKC alpha
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pHACE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_21233 Copy
Species: Homo sapiens
Genetic Insert: EsR1- HRas
Vector Backbone Description: Backbone Size:11100; Vector Backbone:LZRS no LacZ; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_21199 Copy
Species: Homo sapiens
Genetic Insert: HRas
Vector Backbone Description: Backbone Size:11100; Vector Backbone:LZRS no LacZ; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_21198 Copy
Species: Homo sapiens
Genetic Insert: PKC alpha
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pHACE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_21235 Copy
Species: Homo sapiens
Genetic Insert: Mek2
Vector Backbone Description: Backbone Size:11100; Vector Backbone:LZRS-RfA; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_21191 Copy
Species: Homo sapiens
Genetic Insert: Mek1
Vector Backbone Description: Backbone Size:11100; Vector Backbone:LZRS-RfA; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_21196 Copy
Species: Homo sapiens
Genetic Insert: Mek2
Vector Backbone Description: Backbone Size:11100; Vector Backbone:LZRS-RfA; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_21194 Copy
Species: Homo sapiens
Genetic Insert: Mek2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2200; Vector Backbone:pENTR1A; Vector Types:Subclone vector; Bacterial Resistance:Kanamycin
References:
Comments: Constitutive active protein has the following aa sequence deleted - EQQKKRLE, residues 48 to 55.
Proper citation: RRID:Addgene_21211 Copy
Species: Homo sapiens
Genetic Insert: Phosphoglycerate kinase promoter
Vector Backbone Description: Backbone Size:7226; Vector Backbone:SIN18; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments: There are several mismatches between depositor's and Addgene sequence. These mutations do not affect eGFP expression.
Proper citation: RRID:Addgene_21210 Copy
Species: Homo sapiens
Genetic Insert: DUX4
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_21176 Copy
Species: Homo sapiens
Genetic Insert: JMJD3
Vector Backbone Description: Backbone Size:6300; Vector Backbone:pMSCVpuro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_21214 Copy
Species: Homo sapiens
Genetic Insert: PLCdelta
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: This construct encodes a PI(4,5)P2 lipid selective PH domain that can be used as a fluorescent translocation biosensor to monitor changes or local differences in the concentration of plasma membrane PI(4,5)P2 lipids (Stauffer TP, Ahn S, Meyer T; Curr Biol. 1998 8:343-6). It is best used in combination with a second fluorescent protein that uniformly marks the plasma membrane (the last 20 amino acids "CAAX" region of KRas is often used as a PM marker). Relative changes in cell-wide or local plasma membrane PI(4,5)P2 can be determined by using a mask for the PM region and by dividing the two background subtracted PH domain and CAAX images. At high expression levels, this construct interferes with PI(4,5)P2 signaling and alters cortical actin (Raucher D et al Cell. 2000 100:221-8).
Proper citation: RRID:Addgene_21179 Copy
Species: Homo sapiens
Genetic Insert: JMJD3
Vector Backbone Description: Backbone Size:6300; Vector Backbone:pMSCVpuro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_21212 Copy
Species: Homo sapiens
Genetic Insert: E2F transcription factor 1
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5256; Vector Backbone:pGL3-Control; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_21170 Copy
Species: Homo sapiens
Genetic Insert: DUX4
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_21172 Copy
Species: Homo sapiens
Genetic Insert: E2F 3'UTR Mutant
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5256; Vector Backbone:pGL3-Control; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_21171 Copy
Species: Homo sapiens
Genetic Insert: HIF1AN
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_21399 Copy
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