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Species: Homo sapiens
Genetic Insert: RING finger protein 1 (ROC1)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4620; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_20717 Copy
Species: Homo sapiens
Genetic Insert: VHL
Vector Backbone Description: Backbone Marker:Pharmacia; Backbone Size:4959; Vector Backbone:pGex2TK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_20790 Copy
Species: Homo sapiens
Genetic Insert: HOXB4
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3000; Vector Backbone:pSP65; Vector Types:in vitro TNT; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_20986 Copy
Species: Homo sapiens
Genetic Insert: HOXB5
Vector Backbone Description: Backbone Size:2900; Vector Backbone:pECE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_20985 Copy
Species: Homo sapiens
Genetic Insert: HOXB5
Vector Backbone Description: Backbone Size:4820; Vector Backbone:pCMV1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_20983 Copy
Species: Homo sapiens
Genetic Insert: E12 2/5
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_20913 Copy
Species: Homo sapiens
Genetic Insert: HOXB4
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3000; Vector Backbone:pSP65 (?); Vector Types:in vitro trans/trans; Bacterial Resistance:Ampicillin
References:
Comments: Though Addgene could confirm the presence of the insert, we could not confirm the vector. Addgene suggests that you sequence this primer if you order it.
Proper citation: RRID:Addgene_20990 Copy
Species: Homo sapiens
Genetic Insert: MLL
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6500; Vector Backbone:pMSCV-neo; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: This is a control construct testing the newly developed fMLLpl construct for transforming activity in a MLL-ENL fusion.
SmaI/XhoI fragment from pBluescriptII-ENL3' was inserted into a PmlI/XhoI cut pMSCV-flagMLLpl vector. The end plasmid has ~4.18kb MLL + pl remainings + ~0.4kb ENL with remaining pBSII.
Proper citation: RRID:Addgene_20873 Copy
Species: Homo sapiens
Genetic Insert: HOXB6
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6500; Vector Backbone:MSCV; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: Protein does not bind PBX
Has IRES-GFP
Proper citation: RRID:Addgene_20993 Copy
Species: Homo sapiens
Genetic Insert: HOXB6
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6500; Vector Backbone:MSCV; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: Product of N->A mutation is a DNA-binding mutant.
Has IRES-GF
Proper citation: RRID:Addgene_20992 Copy
Species: Homo sapiens
Genetic Insert: Histone cluster 1, H2bb
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: The accession number for H2B.1 sequence X57127 was obtained from Fraser et al., 2005 (https://doi.org/10.1002/gene.20139). The actual DNA was PCR amplified from HEK293 genomic DNA then cloned into pEGFP-N1 as described in Kanda et al., 1998 (https://doi.org/10.1016/s0960-9822(98)70156-3). That H2B.1-EGFP construct was used as template to synthesize the construct here. An EcoRI site in the H2B.1 sequence was silently mutated. The Orange cDNA was obtained from Dr. Roger Tsien.
Proper citation: RRID:Addgene_20969 Copy
Species: Homo sapiens
Genetic Insert: Histone cluster 1, H2bb
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: The accession number for H2B.1 sequence X57127 was obtained from Fraser et al., 2005 (https://doi.org/10.1002/gene.20139). The actual DNA was PCR amplified from HEK293 genomic DNA then cloned into pEGFP-N1 as described in Kanda et al., 1998 (https://doi.org/10.1016/s0960-9822(98)70156-3). That H2B.1-EGFP construct was used as template to synthesize the construct here. An EcoRI site in the H2B.1 sequence was silently mutated. The Venus cDNA was obtained from Dr. Anna-Katerina Hadjantonakis.
Proper citation: RRID:Addgene_20971 Copy
Species: Homo sapiens
Genetic Insert: Histone cluster 1, H2bb
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: The accession number for H2B.1 sequence X57127 was obtained from Fraser et al., 2005 (https://doi.org/10.1002/gene.20139). The actual DNA was PCR amplified from HEK293 genomic DNA then cloned into pEGFP-N1 as described in Kanda et al., 1998 (https://doi.org/10.1016/s0960-9822(98)70156-3). That H2B.1-EGFP construct was used as template to synthesize the construct here. An EcoRI site in the H2B.1 sequence was silently mutated. The Strawberry cDNA was obtained from Dr. Roger Tsien.
Proper citation: RRID:Addgene_20970 Copy
Species: Homo sapiens
Genetic Insert: BAF60b
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2961; Vector Backbone:pBlueScript SK(-); Vector Types:phagemid; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_21035 Copy
Species: Homo sapiens
Genetic Insert: BAF60a
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2961; Vector Backbone:pBlueScript SK(-); Vector Types:phagemid; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_21034 Copy
Species: Homo sapiens
Genetic Insert: alpha Tubulin
Vector Backbone Description: Backbone Size:4684; Vector Backbone:pmEGFP_C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_21039 Copy
Species: Homo sapiens
Genetic Insert: pMyrPalm
Vector Backbone Description: Backbone Size:5904; Vector Backbone:IRES_puro2b; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_21038 Copy
Species: Homo sapiens
Genetic Insert: pMyrPalm
Vector Backbone Description: Backbone Size:4780; Vector Backbone:pmEGFP_C1; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_21037 Copy
Species: Homo sapiens
Genetic Insert: BAF60c
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2961; Vector Backbone:pBlueScript SK(-); Vector Types:phagemid; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_21036 Copy
Species: Homo sapiens
Genetic Insert: HOXC5
Vector Backbone Description: Backbone Size:0; Vector Backbone:pCT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_20999 Copy
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