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Species: Homo sapiens
Genetic Insert: PIK3CB
Vector Backbone Description: Backbone Marker:William Hahn Lab (available at Addgene, #15300); Backbone Size:0; Vector Backbone:pWZL-Neo-Myr-Flag-DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: ORFs were cloned into Gateway compatible pEntry vectors. An LR recombination reaction was performed to move the ORF to pWZL-Neo-Myr-Flag DEST such that the ORF would have 5' myristoylation and flag tags.
This plasmid is part of a kinase library, and the gene has been verified, but has not been fully sequenced for minor mutations. Please see the following link for plasmids from this article that are not part of the kinase library http://www.addgene.org/pubmed/17574021
Proper citation: RRID:Addgene_20573 Copy
Species: Homo sapiens
Genetic Insert: PI4K2B
Vector Backbone Description: Backbone Marker:William Hahn Lab (available at Addgene, #15300); Backbone Size:0; Vector Backbone:pWZL-Neo-Myr-Flag-DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: ORFs were cloned into Gateway compatible pEntry vectors. An LR recombination reaction was performed to move the ORF to pWZL-Neo-Myr-Flag DEST such that the ORF would have 5' myristoylation and flag tags.
This plasmid is part of a kinase library, and the gene has been verified, but has not been fully sequenced for minor mutations. Please see the following link for plasmids from this article that are not part of the kinase library http://www.addgene.org/pubmed/17574021
Proper citation: RRID:Addgene_20572 Copy
Species: Homo sapiens
Genetic Insert: PIK3R5
Vector Backbone Description: Backbone Marker:William Hahn Lab (available at Addgene, #15300); Backbone Size:0; Vector Backbone:pWZL-Neo-Myr-Flag-DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: ORFs were cloned into Gateway compatible pEntry vectors. An LR recombination reaction was performed to move the ORF to pWZL-Neo-Myr-Flag DEST such that the ORF would have 5' myristoylation and flag tags.
This plasmid is part of a kinase library, and the gene has been verified, but has not been fully sequenced for minor mutations. Please see the following link for plasmids from this article that are not part of the kinase library http://www.addgene.org/pubmed/17574021
There is a mutation in the sequence causing S3C and E58G substitution in the aa sequence.
Proper citation: RRID:Addgene_20576 Copy
Species: Homo sapiens
Genetic Insert: NEK6
Vector Backbone Description: Backbone Marker:William Hahn Lab (available at Addgene, #15300); Backbone Size:0; Vector Backbone:pWZL-Neo-Myr-Flag-DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: ORFs were cloned into Gateway compatible pEntry vectors. An LR recombination reaction was performed to move the ORF to pWZL-Neo-Myr-Flag DEST such that the ORF would have 5' myristoylation and flag tags.
This plasmid is part of a kinase library, and the gene has been verified, but has not been fully sequenced for minor mutations. Please see the following link for plasmids from this article that are not part of the kinase library http://www.addgene.org/pubmed/17574021
Proper citation: RRID:Addgene_20548 Copy
Species: Homo sapiens
Genetic Insert: UCK2
Vector Backbone Description: Backbone Marker:William Hahn Lab (available at Addgene, #15300); Backbone Size:0; Vector Backbone:pWZL-Neo-Myr-Flag-DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: ORFs were cloned into Gateway compatible pEntry vectors. An LR recombination reaction was performed to move the ORF to pWZL-Neo-Myr-Flag DEST such that the ORF would have 5' myristoylation and flag tags.
This plasmid is part of a kinase library, and the gene has been verified, but has not been fully sequenced for minor mutations. Please see the following link for plasmids from this article that are not part of the kinase library http://www.addgene.org/pubmed/17574021
Proper citation: RRID:Addgene_20661 Copy
Species: Homo sapiens
Genetic Insert: ITPKB
Vector Backbone Description: Backbone Marker:William Hahn Lab (available at Addgene, #15300); Backbone Size:0; Vector Backbone:pWZL-Neo-Myr-Flag-DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: ORFs were cloned into Gateway compatible pEntry vectors. An LR recombination reaction was performed to move the ORF to pWZL-Neo-Myr-Flag DEST such that the ORF would have 5' myristoylation and flag tags.
This plasmid is part of a kinase library, and the gene has been verified, but has not been fully sequenced for minor mutations. Please see the following link for plasmids from this article that are not part of the kinase library http://www.addgene.org/pubmed/17574021
There is a V3M mutation in the sequence.
Proper citation: RRID:Addgene_20550 Copy
Species: Homo sapiens
Genetic Insert: Moesin
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_20671 Copy
Species: Homo sapiens
Genetic Insert: miR-30
Vector Backbone Description: Backbone Size:3200; Vector Backbone:pCMV-PL; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please see https://pubmed.ncbi.nlm.nih.gov/12554881/ for additional details about this plasmid.
Proper citation: RRID:Addgene_20670 Copy
Species: Homo sapiens
Genetic Insert: Moesin
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_20676 Copy
Species: Homo sapiens
Genetic Insert: CKMT2
Vector Backbone Description: Backbone Marker:William Hahn Lab (available at Addgene, #15300); Backbone Size:0; Vector Backbone:pWZL-Neo-Myr-Flag-DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: ORFs were cloned into Gateway compatible pEntry vectors. An LR recombination reaction was performed to move the ORF to pWZL-Neo-Myr-Flag DEST such that the ORF would have 5' myristoylation and flag tags.
This plasmid is part of a kinase library, and the gene has been verified, but has not been fully sequenced for minor mutations. Please see the following link for plasmids from this article that are not part of the kinase library http://www.addgene.org/pubmed/17574021
Proper citation: RRID:Addgene_20554 Copy
Species: Homo sapiens
Genetic Insert: OXSR1
Vector Backbone Description: Backbone Marker:William Hahn Lab (available at Addgene, #15300); Backbone Size:0; Vector Backbone:pWZL-Neo-Myr-Flag-DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: ORFs were cloned into Gateway compatible pEntry vectors. An LR recombination reaction was performed to move the ORF to pWZL-Neo-Myr-Flag DEST such that the ORF would have 5' myristoylation and flag tags.
This plasmid is part of a kinase library, and the gene has been verified, but has not been fully sequenced for minor mutations. Please see the following link for plasmids from this article that are not part of the kinase library http://www.addgene.org/pubmed/17574021
Proper citation: RRID:Addgene_20553 Copy
Species: Homo sapiens
Genetic Insert: TAOK3
Vector Backbone Description: Backbone Marker:William Hahn Lab (available at Addgene, #15300); Backbone Size:0; Vector Backbone:pWZL-Neo-Myr-Flag-DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: ORFs were cloned into Gateway compatible pEntry vectors. An LR recombination reaction was performed to move the ORF to pWZL-Neo-Myr-Flag DEST such that the ORF would have 5' myristoylation and flag tags.
This plasmid is part of a kinase library, and the gene has been verified, but has not been fully sequenced for minor mutations. Please see the following link for plasmids from this article that are not part of the kinase library http://www.addgene.org/pubmed/17574021
Proper citation: RRID:Addgene_20647 Copy
Species: Homo sapiens
Genetic Insert: SYK
Vector Backbone Description: Backbone Marker:William Hahn Lab (available at Addgene, #15300); Backbone Size:0; Vector Backbone:pWZL-Neo-Myr-Flag-DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: ORFs were cloned into Gateway compatible pEntry vectors. An LR recombination reaction was performed to move the ORF to pWZL-Neo-Myr-Flag DEST such that the ORF would have 5' myristoylation and flag tags.
This plasmid is part of a kinase library, and the gene has been verified, but has not been fully sequenced for minor mutations. Please see the following link for plasmids from this article that are not part of the kinase library http://www.addgene.org/pubmed/17574021
Proper citation: RRID:Addgene_20646 Copy
Species: Homo sapiens
Genetic Insert: TEC
Vector Backbone Description: Backbone Marker:William Hahn Lab (available at Addgene, #15300); Backbone Size:0; Vector Backbone:pWZL-Neo-Myr-Flag-DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: ORFs were cloned into Gateway compatible pEntry vectors. An LR recombination reaction was performed to move the ORF to pWZL-Neo-Myr-Flag DEST such that the ORF would have 5' myristoylation and flag tags.
This plasmid is part of a kinase library, and the gene has been verified, but has not been fully sequenced for minor mutations. Please see the following link for plasmids from this article that are not part of the kinase library http://www.addgene.org/pubmed/17574021
Proper citation: RRID:Addgene_20649 Copy
Species: Homo sapiens
Genetic Insert: STK33
Vector Backbone Description: Backbone Marker:William Hahn Lab (available at Addgene, #15300); Backbone Size:0; Vector Backbone:pWZL-Neo-Myr-Flag-DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: ORFs were cloned into Gateway compatible pEntry vectors. An LR recombination reaction was performed to move the ORF to pWZL-Neo-Myr-Flag DEST such that the ORF would have 5' myristoylation and flag tags.
This plasmid is part of a kinase library, and the gene has been verified, but has not been fully sequenced for minor mutations. Please see the following link for plasmids from this article that are not part of the kinase library http://www.addgene.org/pubmed/17574021
Proper citation: RRID:Addgene_20641 Copy
Species: Homo sapiens
Genetic Insert: PRKAG2
Vector Backbone Description: Backbone Marker:William Hahn Lab (available at Addgene, #15300); Backbone Size:0; Vector Backbone:pWZL-Neo-Myr-Flag-DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: ORFs were cloned into Gateway compatible pEntry vectors. An LR recombination reaction was performed to move the ORF to pWZL-Neo-Myr-Flag DEST such that the ORF would have 5' myristoylation and flag tags.
This plasmid is part of a kinase library, and the gene has been verified, but has not been fully sequenced for minor mutations. Please see the following link for plasmids from this article that are not part of the kinase library http://www.addgene.org/pubmed/17574021
Proper citation: RRID:Addgene_20656 Copy
Species: Homo sapiens
Genetic Insert: TNK2
Vector Backbone Description: Backbone Marker:William Hahn Lab (available at Addgene, #15300); Backbone Size:0; Vector Backbone:pWZL-Neo-Myr-Flag-DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: ORFs were cloned into Gateway compatible pEntry vectors. An LR recombination reaction was performed to move the ORF to pWZL-Neo-Myr-Flag DEST such that the ORF would have 5' myristoylation and flag tags.
This plasmid is part of a kinase library, and the gene has been verified, but has not been fully sequenced for minor mutations. Please see the following link for plasmids from this article that are not part of the kinase library http://www.addgene.org/pubmed/17574021
Proper citation: RRID:Addgene_20654 Copy
Species: Homo sapiens
Genetic Insert: TK1
Vector Backbone Description: Backbone Marker:William Hahn Lab (available at Addgene, #15300); Backbone Size:0; Vector Backbone:pWZL-Neo-Myr-Flag-DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: ORFs were cloned into Gateway compatible pEntry vectors. An LR recombination reaction was performed to move the ORF to pWZL-Neo-Myr-Flag DEST such that the ORF would have 5' myristoylation and flag tags.
This plasmid is part of a kinase library, and the gene has been verified, but has not been fully sequenced for minor mutations. Please see the following link for plasmids from this article that are not part of the kinase library http://www.addgene.org/pubmed/17574021
Proper citation: RRID:Addgene_20652 Copy
Species: Homo sapiens
Genetic Insert: TIE1
Vector Backbone Description: Backbone Marker:William Hahn Lab (available at Addgene, #15300); Backbone Size:0; Vector Backbone:pWZL-Neo-Myr-Flag-DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: ORFs were cloned into Gateway compatible pEntry vectors. An LR recombination reaction was performed to move the ORF to pWZL-Neo-Myr-Flag DEST such that the ORF would have 5' myristoylation and flag tags.
This plasmid is part of a kinase library, and the gene has been verified, but has not been fully sequenced for minor mutations. Please see the following link for plasmids from this article that are not part of the kinase library http://www.addgene.org/pubmed/17574021
Proper citation: RRID:Addgene_20651 Copy
Species: Homo sapiens
Genetic Insert: IRE1
Vector Backbone Description: Backbone Marker:Eric Campeau (Addgene plasmid 17293); Backbone Size:9594; Vector Backbone:pLenti CMV/TO Puro DEST (670-1); Vector Types:Mammalian Expression, Lentiviral, Gateway Cloning; Bacterial Resistance:Ampicillin
References:
Comments: IRE1a cDNA was cut out of pENTR1A with EcoRI and XhoI and ligated into pLenti CMV/TO Puro DEST through Gateway cloning.
Proper citation: RRID:Addgene_20745 Copy
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