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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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YF-Rac1(DN) Resource Report Resource Website 1+ mentions |
RRID:Addgene_20151 | YFP-FKBP-Rac1(DN) | Homo sapiens | Kanamycin | PMID:15908919 | Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:EYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | dominant negative mutation | 2026-07-25 12:44:16 | 2 | |
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YF-Rac1(CA) Resource Report Resource Website 1+ mentions |
RRID:Addgene_20150 | YFP-FKBP-Rac1(CA) | Homo sapiens | Kanamycin | PMID:15908919 | Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:EYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | constitutively active mutation | 2026-07-25 12:44:16 | 1 | |
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pCDNA3 Flag p38 beta2 (agf) Resource Report Resource Website 1+ mentions |
RRID:Addgene_20356 | pDCNA3 Flag p38 beta(agf) | Homo sapiens | Ampicillin | PMID:9430721 | There are two additional mutations in the sequence - Q22L and M198L. These mutations are correct and the sequence still functions normally. | Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Replace Thr 182 and Tyr 184 with Ala and Phe respectively | 2026-07-25 12:44:17 | 3 |
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G608G lamin A minigene Resource Report Resource Website |
RRID:Addgene_20292 | lamin A | Homo sapiens | Kanamycin | PMID:15750600 | To generate the wt lamin A minigene, intron 11 was first introduced between exon 11 and exon 12 in the lamin A cDNA in pEGFP-lamin A using a multi-step PCR reaction. In the fist step a region of the lamin A gene containing the last 156 bp of exon 11, intron 11 and the first 31 bp of exon 12 was amplified by PCR from genomic DNA obtained from HeLa cells, using the primers: LMNA-mini11wtF (5’-GCCCAGGTGGGCGGACCCATC-3’)/LMNA-mini12R (5’-CAGATTACATGATGCTGCAGTTCTG-3’). In the second step, a region of pEGFP-lamin A from the last 37 bp of exon 9 to the mutated region in exon 11 was amplified using the primers LMNA-mini9F/LMNA-mini11wtR (5’-GATGGGTCCGCCCACCTGGGC-3’). In the third step a region of pEGFP-lamin A containing the last 240 bp of exon 12 was amplified using the primers: LMNA-mini12F (5’-CAGAACTGCAGCATCATGTAATCTG-3’)/ GFPvectorR. The PCR products obtained from the first and third steps were mixed and used in a fourth PCR reaction in the presence of the primers LMNA-mini11wtF/GFPvectorR. The obtained PCR product was then mixed with the product obtained from the second step and used in a fifth PCR reaction with the primers: LMNA-mini9F/ GFPvectorR. The final amplification product was cloned into the BsiWI/XbaI site of pEGFP-lamin A to obtain wt pEGFP-lamin Aintr11. To generate the minigene wt pEGFP-lamin Aintr11 was digested with EcoRI and BstWI enzymes to remove a region of lamin A cDNA from exon 1 to the first 91 bp of exon 9, filled with Klenow enzyme and self-ligated. The mutated minigene was generated with the same procedure, substituting primers LMNA-mini11wtF e LMNA-mini11wtR respectively with: LMNA-mini11mutF (5’-GCCCAGGTGGGTGGACCCATC-3’) and LMNA-mini11mut R (5’-GATGGGTCCACCCACCTGGGC-3’). | Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | G606G:GGC>GGT Mutation causes aberrant splicing event, creating a mutant protein, delta50 lamin A, containing a 50 aa internal deletion in its globular tail domain. | 2026-07-25 12:44:17 | 0 |
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mycUBB+1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_20254 | Ubiquitin B +1 transcript | Homo sapiens | Ampicillin | PMID:18796559 | Backbone Size:3300; Vector Backbone:pCMV-Myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-07-25 12:44:17 | 1 | ||
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GST TopBP1 (aa 32-1522) His Resource Report Resource Website 1+ mentions |
RRID:Addgene_20375 | TopBP1 | Homo sapiens | Ampicillin | PMID:19139065 | TopBP1 cDNA KIAA00259 was cloned by Weei-Chin Lin in EcoRI site of pGEX6P1. I added a His6 tag at the 3' end using the oligos TopBPF3 and TopBPR4 for PCR, then digested the PCR product with XhoI and replaced the XhoI fragment from pGEX6P1TopBP1 with the PCR product to generate pGEX6P1TopBP1His. NOTE: This plasmid was originally called full-length, and it is considered to have full length functionality in its original publication from the Sancar lab. | Backbone Marker:Amersham; Backbone Size:4900; Vector Backbone:pGEX6P1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Starts at aa32 | 2026-07-25 12:44:17 | 4 |
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GST TopBP1 D (aa 1-1013) Resource Report Resource Website |
RRID:Addgene_20374 | TopBP1 (1-1013) | Homo sapiens | Ampicillin | PMID:19139065 | Backbone Marker:Amersham; Backbone Size:4900; Vector Backbone:pGEX6P1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | amino acids 1-1013 | 2026-07-25 12:44:17 | 0 | |
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Luc-SIRT1 3'UTR Resource Report Resource Website 1+ mentions |
RRID:Addgene_20379 | SIRT1 | Homo sapiens | Ampicillin | PMID:18755897 | There are mismatches between Addgene's sequence and NCBI sequence for SIRT1 UTR. Deposit is aware of these changes and doesn't think they affect function. | Backbone Size:6470; Vector Backbone:pMIR-REPORT; Vector Types:Luciferase; Bacterial Resistance:Ampicillin | 3'UTR of SIRT1 | 2026-07-25 12:44:17 | 4 |
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pWZL Neo Myr Flag IKBKE Resource Report Resource Website |
RRID:Addgene_20504 | IKBKE | Homo sapiens | Ampicillin | PMID:17574021 | ORFs were cloned into Gateway compatible pEntry vectors. An LR recombination reaction was performed to move the ORF to pWZL-Neo-Myr-Flag DEST such that the ORF would have 5' myristoylation and flag tags. This plasmid is part of a kinase library, and the gene has been verified, but has not been fully sequenced for minor mutations. Please see the following link for plasmids from this article that are not part of the kinase library http://www.addgene.org/pubmed/17574021 | Backbone Marker:William Hahn Lab (available at Addgene, #15300); Backbone Size:0; Vector Backbone:pWZL-Neo-Myr-Flag-DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | 2026-07-25 12:44:18 | 0 | |
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pWZL Neo Myr Flag MOBKL1A Resource Report Resource Website |
RRID:Addgene_20503 | MOBKL1A | Homo sapiens | Ampicillin | PMID:17574021 | ORFs were cloned into Gateway compatible pEntry vectors. An LR recombination reaction was performed to move the ORF to pWZL-Neo-Myr-Flag DEST such that the ORF would have 5' myristoylation and flag tags. This plasmid is part of a kinase library, and the gene has been verified, but has not been fully sequenced for minor mutations. Please see the following link for plasmids from this article that are not part of the kinase library http://www.addgene.org/pubmed/17574021 | Backbone Marker:William Hahn Lab (available at Addgene, #15300); Backbone Size:0; Vector Backbone:pWZL-Neo-Myr-Flag-DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | 2026-07-25 12:44:18 | 0 | |
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pWZL Neo Myr Flag HK2 Resource Report Resource Website 1+ mentions |
RRID:Addgene_20501 | HK2 | Homo sapiens | Ampicillin | PMID:17574021 | ORFs were cloned into Gateway compatible pEntry vectors. An LR recombination reaction was performed to move the ORF to pWZL-Neo-Myr-Flag DEST such that the ORF would have 5' myristoylation and flag tags. This plasmid is part of a kinase library, and the gene has been verified, but has not been fully sequenced for minor mutations. Please see the following link for plasmids from this article that are not part of the kinase library http://www.addgene.org/pubmed/17574021 | Backbone Marker:William Hahn Lab (available at Addgene, #15300); Backbone Size:0; Vector Backbone:pWZL-Neo-Myr-Flag-DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | 2026-07-25 12:44:18 | 4 | |
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pWZL Neo Myr Flag ITPK1 Resource Report Resource Website |
RRID:Addgene_20508 | ITPK1 | Homo sapiens | Ampicillin | PMID:17574021 | ORFs were cloned into Gateway compatible pEntry vectors. An LR recombination reaction was performed to move the ORF to pWZL-Neo-Myr-Flag DEST such that the ORF would have 5' myristoylation and flag tags. This plasmid is part of a kinase library, and the gene has been verified, but has not been fully sequenced for minor mutations. Please see the following link for plasmids from this article that are not part of the kinase library http://www.addgene.org/pubmed/17574021 | Backbone Marker:William Hahn Lab (available at Addgene, #15300); Backbone Size:0; Vector Backbone:pWZL-Neo-Myr-Flag-DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | 2026-07-25 12:44:18 | 0 | |
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pWZL Neo Myr Flag ITK Resource Report Resource Website |
RRID:Addgene_20507 | ITK | Homo sapiens | Ampicillin | PMID:17574021 | ORFs were cloned into Gateway compatible pEntry vectors. An LR recombination reaction was performed to move the ORF to pWZL-Neo-Myr-Flag DEST such that the ORF would have 5' myristoylation and flag tags. This plasmid is part of a kinase library, and the gene has been verified, but has not been fully sequenced for minor mutations. Please see the following link for plasmids from this article that are not part of the kinase library http://www.addgene.org/pubmed/17574021 | Backbone Marker:William Hahn Lab (available at Addgene, #15300); Backbone Size:0; Vector Backbone:pWZL-Neo-Myr-Flag-DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | 2026-07-25 12:44:18 | 0 | |
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pWZL Neo Myr Flag CLK1 Resource Report Resource Website |
RRID:Addgene_20463 | CLK1 | Homo sapiens | Ampicillin | PMID:17574021 | ORFs were cloned into Gateway compatible pEntry vectors. An LR recombination reaction was performed to move the ORF to pWZL-Neo-Myr-Flag DEST such that the ORF would have 5' myristoylation and flag tags. This plasmid is part of a kinase library, and the gene has been verified, but has not been fully sequenced for minor mutations. Please see the following link for plasmids from this article that are not part of the kinase library http://www.addgene.org/pubmed/17574021 | Backbone Marker:William Hahn Lab (available at Addgene, #15300); Backbone Size:0; Vector Backbone:pWZL-Neo-Myr-Flag-DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | 2026-07-25 12:44:18 | 0 | |
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pWZL Neo Myr Flag CKS2 Resource Report Resource Website |
RRID:Addgene_20462 | CKS2 | Homo sapiens | Ampicillin | PMID:17574021 | ORFs were cloned into Gateway compatible pEntry vectors. An LR recombination reaction was performed to move the ORF to pWZL-Neo-Myr-Flag DEST such that the ORF would have 5' myristoylation and flag tags. This plasmid is part of a kinase library, and the gene has been verified, but has not been fully sequenced for minor mutations. Please see the following link for plasmids from this article that are not part of the kinase library http://www.addgene.org/pubmed/17574021 Very short isoform, only 79aa long. | Backbone Marker:William Hahn Lab (available at Addgene, #15300); Backbone Size:0; Vector Backbone:pWZL-Neo-Myr-Flag-DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | 2026-07-25 12:44:18 | 0 | |
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pWZL Neo Myr Flag CSNK1A1L Resource Report Resource Website |
RRID:Addgene_20467 | CSNK1A1L | Homo sapiens | Ampicillin | PMID:17574021 | ORFs were cloned into Gateway compatible pEntry vectors. An LR recombination reaction was performed to move the ORF to pWZL-Neo-Myr-Flag DEST such that the ORF would have 5' myristoylation and flag tags. This plasmid is part of a kinase library, and the gene has been verified, but has not been fully sequenced for minor mutations. Please see the following link for plasmids from this article that are not part of the kinase library http://www.addgene.org/pubmed/17574021 | Backbone Marker:William Hahn Lab (available at Addgene, #15300); Backbone Size:0; Vector Backbone:pWZL-Neo-Myr-Flag-DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | 2026-07-25 12:44:18 | 0 | |
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pWZL Neo Myr Flag CLK3 Resource Report Resource Website |
RRID:Addgene_20465 | CLK3 | Homo sapiens | Ampicillin | PMID:17574021 | ORFs were cloned into Gateway compatible pEntry vectors. An LR recombination reaction was performed to move the ORF to pWZL-Neo-Myr-Flag DEST such that the ORF would have 5' myristoylation and flag tags. This plasmid is part of a kinase library, and the gene has been verified, but has not been fully sequenced for minor mutations. Please see the following link for plasmids from this article that are not part of the kinase library http://www.addgene.org/pubmed/17574021 | Backbone Marker:William Hahn Lab (available at Addgene, #15300); Backbone Size:0; Vector Backbone:pWZL-Neo-Myr-Flag-DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | 2026-07-25 12:44:18 | 0 | |
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pWZL Neo Myr Flag MAP2K6 Resource Report Resource Website |
RRID:Addgene_20515 | MAP2K6 | Homo sapiens | Ampicillin | PMID:17574021 | ORFs were cloned into Gateway compatible pEntry vectors. An LR recombination reaction was performed to move the ORF to pWZL-Neo-Myr-Flag DEST such that the ORF would have 5' myristoylation and flag tags. This plasmid is part of a kinase library, and the gene has been verified, but has not been fully sequenced for minor mutations. Please see the following link for plasmids from this article that are not part of the kinase library http://www.addgene.org/pubmed/17574021 | Backbone Marker:William Hahn Lab (available at Addgene, #15300); Backbone Size:0; Vector Backbone:pWZL-Neo-Myr-Flag-DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | 2026-07-25 12:44:18 | 0 | |
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pWZL Neo Myr Flag MAP2K5 Resource Report Resource Website |
RRID:Addgene_20514 | MAP2K5 | Homo sapiens | Ampicillin | PMID:17574021 | ORFs were cloned into Gateway compatible pEntry vectors. An LR recombination reaction was performed to move the ORF to pWZL-Neo-Myr-Flag DEST such that the ORF would have 5' myristoylation and flag tags. This plasmid is part of a kinase library, and the gene has been verified, but has not been fully sequenced for minor mutations. Please see the following link for plasmids from this article that are not part of the kinase library http://www.addgene.org/pubmed/17574021 | Backbone Marker:William Hahn Lab (available at Addgene, #15300); Backbone Size:0; Vector Backbone:pWZL-Neo-Myr-Flag-DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | 2026-07-25 12:44:18 | 0 | |
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pWZL Neo Myr Flag LIMK2 Resource Report Resource Website |
RRID:Addgene_20513 | LIMK2 | Homo sapiens | Ampicillin | PMID:17574021 | ORFs were cloned into Gateway compatible pEntry vectors. An LR recombination reaction was performed to move the ORF to pWZL-Neo-Myr-Flag DEST such that the ORF would have 5' myristoylation and flag tags. This plasmid is part of a kinase library, and the gene has been verified, but has not been fully sequenced for minor mutations. Please see the following link for plasmids from this article that are not part of the kinase library http://www.addgene.org/pubmed/17574021 | Backbone Marker:William Hahn Lab (available at Addgene, #15300); Backbone Size:0; Vector Backbone:pWZL-Neo-Myr-Flag-DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | 2026-07-25 12:44:18 | 0 |
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