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Species: Homo sapiens
Genetic Insert: NRas
Vector Backbone Description: Backbone Marker:Ohlfest Lab; Backbone Size:5607; Vector Backbone:colE1/ampR; Vector Types:Transposon; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_20205 Copy
Species: Homo sapiens
Genetic Insert: cIAP1 3'UTR
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: The 3′UTR was generated by PCR amplification from a liver cDNA library (Invitrogen) and the expression plasmid pMC-cIAP1-FL was constructed by inserting the 3′UTR of cIAP1 downstream of CAT in the pMC construct.
Proper citation: RRID:Addgene_20209 Copy
Species: Homo sapiens
Genetic Insert: CFP-FKBP
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:ECFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_20160 Copy
Species: Homo sapiens
Genetic Insert: src homology domain 3
Vector Backbone Description: Backbone Marker:novagen; Backbone Size:4500; Vector Backbone:pRSF-1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_20177 Copy
Species: Homo sapiens
Genetic Insert: TNFa 3' UTR
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: The 3′UTR was generated by PCR amplification from a liver cDNA library (Invitrogen). and the expression plasmids pMC-TNFα was constructed by inserting the 3′UTR of TNFα downstream of CAT in the pMC construct.
Proper citation: RRID:Addgene_20210 Copy
Species: Homo sapiens
Genetic Insert: src homology domain 3
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:4500; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Gene 1998 224 45-52
Proper citation: RRID:Addgene_20176 Copy
Species: Homo sapiens
Genetic Insert: pEX-CMV-SP-YFP-STM2(15-746AA)-EF hand 3 mut (K83A,D84N,G86D)
Vector Backbone Description: Backbone Size:0; Vector Backbone:Gateway destination vector; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: STIM2 also has P38L, V222I, and E575V, as described in the paper's supplemental material. These mutations do not affect function.
Proper citation: RRID:Addgene_20174 Copy
Species: Homo sapiens
Genetic Insert: 3xFKBP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5000; Vector Backbone:pcDNA3.1/Zeo(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_20213 Copy
Species: Homo sapiens
Genetic Insert: C-CrkII nSH3
Vector Backbone Description: Backbone Marker:novagen; Backbone Size:4500; Vector Backbone:pRSF-1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: Gene 1998 224 45-52
Proper citation: RRID:Addgene_20178 Copy
Species: Homo sapiens
Genetic Insert: cell division cycle 42 isoform 1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_20142 Copy
Species: Homo sapiens
Genetic Insert: YFP-FRB (YR)
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:EYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_20148 Copy
Species: Homo sapiens
Genetic Insert: Lyn-linker-FRB (LDR)
Vector Backbone Description: Backbone Marker:ARIAD Pharmaceuticals; Backbone Size:0; Vector Backbone:pC4-RHE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_20147 Copy
Species: Homo sapiens
Genetic Insert: YFP-FKBP-Tiam1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:EYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_20154 Copy
Species: Homo sapiens
Genetic Insert: YFP-FKBP-Rac1(DN)
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:EYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_20151 Copy
Species: Homo sapiens
Genetic Insert: YFP-FKBP-Rac1(CA)
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:EYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_20150 Copy
Species: Homo sapiens
Genetic Insert: pDCNA3 Flag p38 beta(agf)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: There are two additional mutations in the sequence - Q22L and M198L. These mutations are correct and the sequence still functions normally.
Proper citation: RRID:Addgene_20356 Copy
Species: Homo sapiens
Genetic Insert: lamin A
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: To generate the wt lamin A minigene, intron 11 was first introduced between exon 11 and exon 12 in the lamin A cDNA in pEGFP-lamin A using a multi-step PCR reaction. In the fist step a region of the lamin A gene containing the last 156 bp of exon 11, intron 11 and the first 31 bp of exon 12 was amplified by PCR from genomic DNA obtained from HeLa cells, using the primers: LMNA-mini11wtF (5’-GCCCAGGTGGGCGGACCCATC-3’)/LMNA-mini12R (5’-CAGATTACATGATGCTGCAGTTCTG-3’). In the second step, a region of pEGFP-lamin A from the last 37 bp of exon 9 to the mutated region in exon 11 was amplified using the primers LMNA-mini9F/LMNA-mini11wtR (5’-GATGGGTCCGCCCACCTGGGC-3’). In the third step a region of pEGFP-lamin A containing the last 240 bp of exon 12 was amplified using the primers: LMNA-mini12F (5’-CAGAACTGCAGCATCATGTAATCTG-3’)/ GFPvectorR. The PCR products obtained from the first and third steps were mixed and used in a fourth PCR reaction in the presence of the primers LMNA-mini11wtF/GFPvectorR. The obtained PCR product was then mixed with the product obtained from the second step and used in a fifth PCR reaction with the primers: LMNA-mini9F/ GFPvectorR. The final amplification product was cloned into the BsiWI/XbaI site of pEGFP-lamin A to obtain wt pEGFP-lamin Aintr11. To generate the minigene wt pEGFP-lamin Aintr11 was digested with EcoRI and BstWI enzymes to remove a region of lamin A cDNA from exon 1 to the first 91 bp of exon 9, filled with Klenow enzyme and self-ligated. The mutated minigene was generated with the same procedure, substituting primers LMNA-mini11wtF e LMNA-mini11wtR respectively with: LMNA-mini11mutF (5’-GCCCAGGTGGGTGGACCCATC-3’) and LMNA-mini11mut R (5’-GATGGGTCCACCCACCTGGGC-3’).
Proper citation: RRID:Addgene_20292 Copy
Species: Homo sapiens
Genetic Insert: Ubiquitin B +1 transcript
Vector Backbone Description: Backbone Size:3300; Vector Backbone:pCMV-Myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_20254 Copy
Species: Homo sapiens
Genetic Insert: TopBP1
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4900; Vector Backbone:pGEX6P1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: TopBP1 cDNA KIAA00259 was cloned by Weei-Chin Lin in EcoRI site of pGEX6P1. I added a His6 tag at the 3' end using the oligos TopBPF3 and TopBPR4 for PCR, then digested the PCR product with XhoI and replaced the XhoI fragment from pGEX6P1TopBP1 with the PCR product to generate pGEX6P1TopBP1His.
NOTE: This plasmid was originally called full-length, and it is considered to have full length functionality in its original publication from the Sancar lab.
Proper citation: RRID:Addgene_20375 Copy
Species: Homo sapiens
Genetic Insert: TopBP1 (1-1013)
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4900; Vector Backbone:pGEX6P1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_20374 Copy
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