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Species: Homo sapiens
Genetic Insert: FKBP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: murine VH chain signal peptide followed by a HA
tag and one repeat of FKBP
Proper citation: RRID:Addgene_20220 Copy
Species: Homo sapiens
Genetic Insert: FRB
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: murine VH chain signal peptide followed by a HA
tag and one repeat of FRB
Proper citation: RRID:Addgene_20225 Copy
Species: Homo sapiens
Genetic Insert: 2xFRB
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: murine VH chain signal peptide followed by a HA
tag and two repeats of FRB
Proper citation: RRID:Addgene_20224 Copy
Species: Homo sapiens
Genetic Insert: 3xFKBP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: murine VH chain signal peptide followed by a HA
tag and three repeats of FKBP
Proper citation: RRID:Addgene_20222 Copy
Species: Homo sapiens
Genetic Insert: RalA
Vector Backbone Description: Backbone Size:9600; Vector Backbone:pPs-neo; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: pPs-neo vector based on psp64
Proper citation: RRID:Addgene_20182 Copy
Species: Homo sapiens
Genetic Insert: RalA
Vector Backbone Description: Backbone Size:9600; Vector Backbone:pPs-neo; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: pPs-neo vector based on psp64
Proper citation: RRID:Addgene_20181 Copy
Species: Homo sapiens
Genetic Insert: TNF receptor-associated factor 2
Vector Backbone Description: Backbone Size:6203; Vector Backbone:pLPCX; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_20230 Copy
Species: Homo sapiens
Genetic Insert: NRas
Vector Backbone Description: Backbone Marker:Ohlfest Lab; Backbone Size:5607; Vector Backbone:colE1/ampR; Vector Types:Transposon; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_20205 Copy
Species: Homo sapiens
Genetic Insert: cIAP1 3'UTR
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: The 3′UTR was generated by PCR amplification from a liver cDNA library (Invitrogen) and the expression plasmid pMC-cIAP1-FL was constructed by inserting the 3′UTR of cIAP1 downstream of CAT in the pMC construct.
Proper citation: RRID:Addgene_20209 Copy
Species: Homo sapiens
Genetic Insert: CFP-FKBP
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:ECFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_20160 Copy
Species: Homo sapiens
Genetic Insert: src homology domain 3
Vector Backbone Description: Backbone Marker:novagen; Backbone Size:4500; Vector Backbone:pRSF-1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_20177 Copy
Species: Homo sapiens
Genetic Insert: TNFa 3' UTR
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: The 3′UTR was generated by PCR amplification from a liver cDNA library (Invitrogen). and the expression plasmids pMC-TNFα was constructed by inserting the 3′UTR of TNFα downstream of CAT in the pMC construct.
Proper citation: RRID:Addgene_20210 Copy
Species: Homo sapiens
Genetic Insert: src homology domain 3
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:4500; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Gene 1998 224 45-52
Proper citation: RRID:Addgene_20176 Copy
Species: Homo sapiens
Genetic Insert: pEX-CMV-SP-YFP-STM2(15-746AA)-EF hand 3 mut (K83A,D84N,G86D)
Vector Backbone Description: Backbone Size:0; Vector Backbone:Gateway destination vector; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: STIM2 also has P38L, V222I, and E575V, as described in the paper's supplemental material. These mutations do not affect function.
Proper citation: RRID:Addgene_20174 Copy
Species: Homo sapiens
Genetic Insert: 3xFKBP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5000; Vector Backbone:pcDNA3.1/Zeo(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_20213 Copy
Species: Homo sapiens
Genetic Insert: C-CrkII nSH3
Vector Backbone Description: Backbone Marker:novagen; Backbone Size:4500; Vector Backbone:pRSF-1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: Gene 1998 224 45-52
Proper citation: RRID:Addgene_20178 Copy
Species: Homo sapiens
Genetic Insert: cell division cycle 42 isoform 1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_20142 Copy
Species: Homo sapiens
Genetic Insert: YFP-FRB (YR)
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:EYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_20148 Copy
Species: Homo sapiens
Genetic Insert: Lyn-linker-FRB (LDR)
Vector Backbone Description: Backbone Marker:ARIAD Pharmaceuticals; Backbone Size:0; Vector Backbone:pC4-RHE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_20147 Copy
Species: Homo sapiens
Genetic Insert: YFP-FKBP-Tiam1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:EYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_20154 Copy
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