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Species: Homo sapiens
Genetic Insert: APC11 (Anaphase promoting complex subunit 11)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5437; Vector Backbone:pcDNA3-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_19900 Copy
Species: Homo sapiens
Genetic Insert: RBJ 3'UTR
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:6249; Vector Backbone:psiCHECK-2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_19866 Copy
Species: Homo sapiens
Genetic Insert: F-box and WD repeat domain containing 5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5437; Vector Backbone:pcDNA3-myc3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_19905 Copy
Species: Homo sapiens
Genetic Insert: CUL4A (cullin 4A)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5437; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: The CUL4A insert in this plasmid contains a K644R substitution when compared to GenBank reference sequence NM_001008895.
Proper citation: RRID:Addgene_19951 Copy
Species: Homo sapiens
Genetic Insert: cullin 1 delta N53
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_19950 Copy
Species: Homo sapiens
Genetic Insert: CUL4A (cullin 4A)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5437; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_19953 Copy
Species: Homo sapiens
Genetic Insert: 14-3-3 Protein Beta
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5437; Vector Backbone:pcDNA3-myc3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_19957 Copy
Species: Homo sapiens
Genetic Insert: Annexin V
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:4606; Vector Backbone:pET12a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_19961 Copy
Species: Homo sapiens
Genetic Insert: D2 short variant-monomeric Venus
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_19966 Copy
Species: Homo sapiens
Genetic Insert: D2 short variant-C terminal Venus
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_19968 Copy
Species: Homo sapiens
Genetic Insert: cullin 2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5437; Vector Backbone:pcDNA3-myc3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_19892 Copy
Species: Homo sapiens
Genetic Insert: cullin 3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5512; Vector Backbone:pcDNA3-myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Y201C mutation detected in Addgene sequence. Xiong lab says that the plasmid used in the publication also had Y201C mutation and it is not known to affect function.
Proper citation: RRID:Addgene_19893 Copy
Species: Homo sapiens
Genetic Insert: cullin 1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5437; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: When we made this plasmid, we put a Nco1 site at the start codon (original cDNA was CAATGTCG but they mutated to CcATGgCG). It made an S2A mutation. There is also a K689E mutation. These mutations have no effect on protein function.
Proper citation: RRID:Addgene_19896 Copy
Species: Homo sapiens
Genetic Insert: cullin 5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5437; Vector Backbone:pcDNA3-myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_19895 Copy
Species: Homo sapiens
Genetic Insert: APC11
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4620; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_19898 Copy
Species: Homo sapiens
Genetic Insert: cyclin E2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4620; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_19935 Copy
Species: Homo sapiens
Genetic Insert: CDKN1B cyclin-dependent kinase inhibitor 1B
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5486; Vector Backbone:pcDNA3-myc3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_19937 Copy
Species: Homo sapiens
Genetic Insert: cullin 1 K720A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_19939 Copy
Species: Homo sapiens
Genetic Insert: cullin 1 Y42A/M43A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: flag tag is a variant: YPYDLPDYA
S2A mutation present but does not appear to affect function
Proper citation: RRID:Addgene_19940 Copy
Species: Homo sapiens
Genetic Insert: cullin 1 delta C22
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_19942 Copy
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