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Species: Homo sapiens
Genetic Insert: HNF1B
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5150; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression, Flp/FRT; Bacterial Resistance:Ampicillin
References:
Comments: for Flp mediated site directed integration, tetracycline inducibilty
Proper citation: RRID:Addgene_31102 Copy
Species: Homo sapiens
Genetic Insert: HNF1B
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5150; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression, Flp/FRT; Bacterial Resistance:Ampicillin
References:
Comments: for Flp mediated site directed integration, tetracycline inducibilty
Please note that there are some small discrepancies between Addgene's quality control sequence and the assembled sequence from the depositor. These discrepancies should not affect the function of the plasmid.
Proper citation: RRID:Addgene_31101 Copy
Species: Homo sapiens
Genetic Insert: HNF1B
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5150; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression, Flp/FRT; Bacterial Resistance:Ampicillin
References:
Comments: for Flp mediated site directed integration, tetracycline inducibilty
Proper citation: RRID:Addgene_31103 Copy
Species: Homo sapiens
Genetic Insert: HNF4A P2 promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4800; Vector Backbone:pGL3-basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_31062 Copy
Species: Homo sapiens
Genetic Insert: osteopontin promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4800; Vector Backbone:pGL3-Basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments: promoter luciferase reporter
Please note that there are some small discrepancies between Addgene's quality control sequence and the assembled sequence from the depositor. These discrepancies should not affect the function of the plasmid.
Proper citation: RRID:Addgene_31106 Copy
Species: Homo sapiens
Genetic Insert: FXYD2
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4800; Vector Backbone:pGL3-basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_31056 Copy
Species: Homo sapiens
Genetic Insert: Dynactin-4
Vector Backbone Description: Backbone Size:4000; Vector Backbone:Modified pEGFP-N1 (see note); Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: For the preparation of HA-dynactin-4, GFP was first cut out of pEGFP-N1 vector between HindIII and NotI, and in its place, we introduced a triple HA sequence that was synthesized by PCR and ligated into the vector. To a recombinant ORF sequence of dynactin-4, we added by PCR a 5′ XhoI site, and a HindIII site on its 3′ end. The dynactin-4 construct was then cloned into the modified 3XHA-containing vector.
Proper citation: RRID:Addgene_31058 Copy
Species: Homo sapiens
Genetic Insert: HNF4A P2 promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4800; Vector Backbone:pGL3-basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments: Please note that there are some small discrepancies between Addgene's quality control sequence and the assembled sequence from the depositor. These discrepancies should not affect the function of the plasmid.
Proper citation: RRID:Addgene_31052 Copy
Species: Homo sapiens
Genetic Insert: MEK
Vector Backbone Description: Backbone Size:2503; Vector Backbone:pME; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
References:
Comments: Middle entry clone.
Proper citation: RRID:Addgene_31166 Copy
Species: Homo sapiens
Genetic Insert: MITF-M
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4300; Vector Backbone:pCMV-TAG4A; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_31152 Copy
Species: Homo sapiens
Genetic Insert: MITF-M
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4300; Vector Backbone:pCMV-TAG4A; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_31154 Copy
Species: Homo sapiens
Genetic Insert: MITF-M
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4300; Vector Backbone:pCMV-TAG4A; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_31151 Copy
Species: Homo sapiens
Genetic Insert: ADAM7
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_31141 Copy
Species: Homo sapiens
Genetic Insert: ADAM29
Vector Backbone Description: Backbone Marker:Systems Biosciences Inc; Backbone Size:6600; Vector Backbone:pCDF1-EF1-MCS2-Puromycin; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_31145 Copy
Species: Homo sapiens
Genetic Insert: HNF4A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5150; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression, Flp/FRT; Bacterial Resistance:Ampicillin
References:
Comments: for Flp mediated site directed integration, tetracycline inducibilty by Tet operator in CMV promoter shortened to -218.
Corresponding wild type (full length) CMV promoter construct in FR_HNF4A2 (Thomas et al 2004).
Proper citation: RRID:Addgene_31090 Copy
Species: Homo sapiens
Genetic Insert: HNF4A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5150; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression, Flp/FRT; Bacterial Resistance:Ampicillin
References:
Comments: for Flp mediated site directed integration, tetracycline inducibilty by Tet operator in CMV promoter shortened to -138.
Corresponding wild type (full length) CMV promoter construct in FR_HNF4A2 (Thomas et al 2004).
Proper citation: RRID:Addgene_31091 Copy
Species: Homo sapiens
Genetic Insert: HNF4A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5150; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression, Flp/FRT; Bacterial Resistance:Ampicillin
References:
Comments: for Flp mediated site directed integration, tetracycline inducibilty by Tet operator in P2 promoter of HNF4A, shield inducibility by destabilizing domain FKBP L106P
Proper citation: RRID:Addgene_31095 Copy
Species: Homo sapiens
Genetic Insert: FLAG-XBP1u-FLuc
Vector Backbone Description: Vector Backbone:pTriEx-4; Vector Types:Mammalian Expression, Bacterial Expression, Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments: EV71 5′ UTR between Renilla and firefly luciferase.
Proper citation: RRID:Addgene_31239 Copy
Species: Homo sapiens
Genetic Insert: CD4-tdTom
Vector Backbone Description: Backbone Size:10193; Vector Backbone:pAPIC-HIH; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: Gateway destination vector for cloning any enhancer to drive expression of membrane marker CD4-tdTom
The depositing laboratory recommends growing bacteria either on LB plates with 80 ug/ml Carbenicillin or in LB liquid media with 60 ug/ml Carbenicillin (a semi-synthetic ampicillin analog)
Proper citation: RRID:Addgene_31222 Copy
Species: Homo sapiens
Genetic Insert: C-terminus of Firefly Luciferase and FK506-binding protein 12
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5840; Vector Backbone:pEF6-V5/HIS TOPO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_31184 Copy
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