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Species: Homo sapiens
Genetic Insert: p300
Vector Backbone Description: Backbone Size:4800; Vector Backbone:pCMVb; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_30490 Copy
Species: Homo sapiens
Genetic Insert: BCL2-associated X protein
Vector Backbone Description: Backbone Size:4700; Vector Backbone:C3-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_30533 Copy
Species: Homo sapiens
Genetic Insert: HDAC6
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_30482 Copy
Species: Homo sapiens
Genetic Insert: HDAC6
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_30484 Copy
Species: Homo sapiens
Genetic Insert: HDAC6
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_30483 Copy
Species: Homo sapiens
Genetic Insert: HDAC4
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_30486 Copy
Species: Homo sapiens
Genetic Insert: p300
Vector Backbone Description: Backbone Size:4800; Vector Backbone:pCMVb; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_30489 Copy
Species: Homo sapiens
Genetic Insert: Barkor
Vector Backbone Description: Backbone Marker:Agilent Technologies; Backbone Size:4300; Vector Backbone:phrGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_30500 Copy
Species: Homo sapiens
Genetic Insert: alpha 5 integrin
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4300; Vector Backbone:pEGFP-N3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_30450 Copy
Species: Homo sapiens
Genetic Insert: reticulon 3
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pcDNA3.1/myc-His(-)B (invitrogen); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_31087 Copy
Species: Homo sapiens
Genetic Insert: ACE2 promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4800; Vector Backbone:pGL3-Basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments: promoter luciferase reporter
Please note that there are some small discrepancies between Addgene's quality control sequence and the assembled sequence from the depositor. These discrepancies should not affect the function of the plasmid.
Proper citation: RRID:Addgene_31111 Copy
Species: Homo sapiens
Genetic Insert: euchromatic histone-lysine N-methyltransferase 2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6963; Vector Backbone:pLenti6/V5-D-TOPO; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments: Spektor TM, Rice JC. Identification and characterization of posttranslational modification-specific binding proteins in vivo by mammalian tethered catalysis. Proc Natl Acad Sci U S A. 2009 Sep 1;106(35):14808-13. Epub 2009 Aug 18.
Proper citation: RRID:Addgene_31113 Copy
Species: Homo sapiens
Genetic Insert: ACE2 promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4800; Vector Backbone:pGL3-Basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments: promoter luciferase reporter
Please note that there are some small discrepancies between Addgene's quality control sequence and the assembled sequence from the depositor. These discrepancies should not affect the function of the plasmid.
Proper citation: RRID:Addgene_31112 Copy
Species: Homo sapiens
Genetic Insert: RalBP1 shRNA
Vector Backbone Description: Backbone Marker:Oligoengine; Backbone Size:6349; Vector Backbone:pSUPER.retro.puro; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
References:
Comments: shRNA RalBP1 sequence: 5′-GTAGAGAGGACCATGATGT-3'
Proper citation: RRID:Addgene_31115 Copy
Species: Homo sapiens
Genetic Insert: HNF4A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5137; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: for Flp mediated site directed integration, tetracycline inducibility
Proper citation: RRID:Addgene_31114 Copy
Species: Homo sapiens
Genetic Insert: beta2-spectrin
Vector Backbone Description: Backbone Size:4000; Vector Backbone:Modified pEGFP-N1 (see note); Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: This construct DOES NOT contain EGFP- the EGFP has been removed from the pEGFP-N1 backbone.
The following "mutations" have been located in this construct. The Bennett lab believes they are due to incorrect sequence data originally entered into NCBI; however, please be aware of these in case you are designing primers or doing mutagenesis:
Derivations from published sequence:
2.59 c-->t (silent)
4.23 c-->g (H-->D)
5.99 g-->c (R-->T)
6.3 g-->a (silent)
Please refer to the Addgene NGS result for the fully validated sequence.
Proper citation: RRID:Addgene_31070 Copy
Species: Homo sapiens
Genetic Insert: TatSF1 shRNA
Vector Backbone Description: Backbone Marker:Oligoengine; Backbone Size:3176; Vector Backbone:pSuper; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin
References:
Comments: shRNA TatSF1 Target: GAGTCAGATGACAAGGAAG
NB: This shRNA for TatSF1 and Plasmid #31120 both work very well.
Proper citation: RRID:Addgene_31119 Copy
Species: Homo sapiens
Genetic Insert: HNF4A P2 promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4800; Vector Backbone:pGL3-basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_31065 Copy
Species: Homo sapiens
Genetic Insert: HNF4A P2 promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4800; Vector Backbone:pGL3-basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_31064 Copy
Species: Homo sapiens
Genetic Insert: HNF4A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5137; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: for Flp mediated site directed integration, tetracycline inducibilty
Proper citation: RRID:Addgene_31100 Copy
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