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Species: Homo sapiens
Genetic Insert: MSL1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5100; Vector Backbone:pcDNA5FRT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: An A87V mutation is present, it does not effect the function of the plasmid.
Proper citation: RRID:Addgene_29762 Copy
Species: Homo sapiens
Genetic Insert: Amyloid Precursor Like Protein 1
Vector Backbone Description: Backbone Size:1380; Vector Backbone:pCAX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_30141 Copy
Species: Homo sapiens
Genetic Insert: Amyloid Precursor Protein
Vector Backbone Description: Backbone Size:1380; Vector Backbone:pCAX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_30143 Copy
Species: Homo sapiens
Genetic Insert: human APP 695 Swedish/Indiana mutation
Vector Backbone Description: Backbone Size:4731; Vector Backbone:pCAX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_30145 Copy
Species: Homo sapiens
Genetic Insert: Amyloid Precursor Protein
Vector Backbone Description: Backbone Size:1380; Vector Backbone:pCAX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_30144 Copy
Species: Homo sapiens
Genetic Insert: Amyloid Precursor Protein - soluble
Vector Backbone Description: Backbone Size:1380; Vector Backbone:pCAX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_30147 Copy
Species: Homo sapiens
Genetic Insert: APP C99
Vector Backbone Description: Backbone Size:4731; Vector Backbone:pCAX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: The insert contains an additional 83 amino acids of unknown composition on the n-terminus. It is unknown whether these amino acids affect plasmid function.
Proper citation: RRID:Addgene_30146 Copy
Species: Homo sapiens
Genetic Insert: Amyloid Precursor Protein - soluble
Vector Backbone Description: Backbone Size:1380; Vector Backbone:pCAX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_30149 Copy
Species: Homo sapiens
Genetic Insert: Amyloid Precursor Protein
Vector Backbone Description: Vector Backbone:pCAX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_30138 Copy
Species: Homo sapiens
Genetic Insert: Amyloid Precursor Protein
Vector Backbone Description: Backbone Size:1380; Vector Backbone:pCAX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_30137 Copy
Species: Homo sapiens
Genetic Insert: WASP-GBD
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:5006; Vector Backbone:pGEX-KG; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_30113 Copy
Species: Homo sapiens
Genetic Insert: rhVitronectin without deletions
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:4607; Vector Backbone:pET-3c; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Note that the first 19 amino acids are the signal peptide for VTN, which is cleaved off on the mature protein during normally protein synthesis in mammalian cells. That means wild type mature VTN starts from aa20. None of the four constructs from this paper have amino acids 1-19.
Proper citation: RRID:Addgene_30223 Copy
Species: Homo sapiens
Genetic Insert: rhVitronectin with N and C terminus cleavages
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:4607; Vector Backbone:pET-3c; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Note that the first 19 amino acids are the signal peptide for VTN, which is cleaved off on the mature protein during normally protein synthesis in mammalian cells. That means wild type mature VTN starts from aa20. None of the four constructs from this paper have amino acids 1-19.
Proper citation: RRID:Addgene_30226 Copy
Species: Homo sapiens
Genetic Insert: IQGAP1
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4948; Vector Backbone:pGEX-2T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_30107 Copy
Species: Homo sapiens
Genetic Insert: GLIS1
Vector Backbone Description: Backbone Marker:Shinya Yamanaka; Backbone Size:4700; Vector Backbone:pMXs-gw; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: pMXs is from Dr. Toshio Kitamura of the University of Tokyo, the Institute of Medical Science. If you use this plasmid in a paper, please cite: Retrovirus-mediated gene transfer and expression cloning: powerful tools in functional genomics. Exp Hematol. 2003 Nov;31(11):1007-14. Kitamura T, Koshino Y, Shibata F, Oki T, Nakajima H, Nosaka T, Kumagai H. Gateway casette is from Invitrogen Corporation.
IMPORTANT NOTE: This plasmid does not match the canonical GLIS1 sequence. For a more recent GLIS1 plasmid from the Yamanaka lab, please proceed to the following page: www.addgene.org/33274
Proper citation: RRID:Addgene_30166 Copy
Species: Homo sapiens
Genetic Insert: Zonula Occludens-1 actin-binding region
Vector Backbone Description: Backbone Size:4735; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_30315 Copy
Species: Homo sapiens
Genetic Insert: Zonula Occludens-1
Vector Backbone Description: Backbone Size:6189; Vector Backbone:pCB6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: pCB6 expression vector originally from Ann Hubbard, Johns Hopkins University. Contains:
CMV promoter, hGH terminator
SV40 polyA signal
pBR322 ori
Proper citation: RRID:Addgene_30317 Copy
Species: Homo sapiens
Genetic Insert: PTEN-R11A
Vector Backbone Description: Backbone Size:9000; Vector Backbone:pHR-SIN; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_30383 Copy
Species: Homo sapiens
Genetic Insert: PTEN-K6A
Vector Backbone Description: Backbone Size:9000; Vector Backbone:pHR-SIN; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_30382 Copy
Species: Homo sapiens
Genetic Insert: PTEN-T383D
Vector Backbone Description: Backbone Size:9000; Vector Backbone:pHR-SIN; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_30381 Copy
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