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Species: Saccharomyces cerevisiae
Genetic Insert: ANP1
Vector Backbone Description: Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_177100 Copy
Species: Other
Genetic Insert: P19 suppressor of gene silencing from Tomato Bushy Stunt Virus
Vector Backbone Description: Vector Backbone:pICH47732; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_177069 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MNN9
Vector Backbone Description: Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_177105 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: NHX1
Vector Backbone Description: Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_177107 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: STE13
Vector Backbone Description: Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_177106 Copy
Species: Other
Genetic Insert: loganic acid O-methyltransferase (CrLAMT) from Catharanthus roseus
Vector Backbone Description: Vector Backbone:pICH47772 ; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_177060 Copy
Species: Other
Genetic Insert: secologanin synthase (CrSLS1) from Catharanthus roseus
Vector Backbone Description: Vector Backbone:pICH47781 ; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_177061 Copy
Species: Other
Genetic Insert: tryptophan decarboxylase (CrTDC) from Catharanthus roseus
Vector Backbone Description: Vector Backbone:pICH47732 ; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_177063 Copy
Species: Other
Genetic Insert: strictosidine synthase (CrSTR) from Catharanthus roseus
Vector Backbone Description: Vector Backbone:pICH47742 ; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_177066 Copy
Species: Other
Genetic Insert: strictosidine synthase (CrSTR) from Catharanthus roseus
Vector Backbone Description: Vector Backbone:pICH47742 ; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_177065 Copy
Species: Rattus norvegicus
Genetic Insert: T alpha 1p
Vector Backbone Description: Backbone Size:0; Vector Backbone:na; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_17707 Copy
Species: Homo sapiens
Genetic Insert: rent1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_17708 Copy
Species: Other
Genetic Insert: loganic acid O-methyltransferase (CrLAMT) from Catharanthus roseus
Vector Backbone Description: Vector Backbone:pICH47772 ; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_177059 Copy
Species: Other
Genetic Insert: 7-deoxyloganic acid hydroxylase (Cr7-DLH) from Catharanthus roseus
Vector Backbone Description: Vector Backbone:pICH47761 ; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_177058 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:7600; Vector Backbone:pCLE; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: pCLE includes a modified 5' LTR (CMV/MLV) to obtain higher titers, as well as the Xenopus EF1alpha enhancer/promoter to improve vector expression in the brain. In addition, the vector is dicistronic and utilizes an IRES to allow translation of the reporter gene, human placental alkaline phosphatase (PLAP).
Proper citation: RRID:Addgene_17703 Copy
Species: Other
Genetic Insert: iridoid oxidase; CYP76A26 (CrIO) from Catharanthus roseus
Vector Backbone Description: Vector Backbone:pICH47742 ; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_177053 Copy
Species:
Genetic Insert: CBFRE
Vector Backbone Description: Backbone Size:3600; Vector Backbone:na; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Ampicillin
References:
Comments: CBFRE: four CBF1-binding elements from EBV (see Hsieh MCB 1996, 16:952) and the basal simian virus 40 (SV40) promoter upstream of EGFP. The sequence of the CBF1 binding element is GATCTGGAGTAACACGCCGTGGGAAAAAATTTATG. The binding site within this sequence is GTGGGAA.
A SmaI+XbaI double digest should yield 3.6kb and approx. 1.2kb bands. See Reviews link in right-hand column to view Addgene's diagnostic digest of this plasmid.
Proper citation: RRID:Addgene_17705 Copy
Species: Other
Genetic Insert: 7-deoxyloganetic acid glucosyl transferase (Cr7-DLGT) from Catharanthus roseus
Vector Backbone Description: Vector Backbone:pICH47751 ; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_177055 Copy
Species: Other
Genetic Insert: iridoid oxidase; CYP76A26 (CrIO) from Catharanthus roseus
Vector Backbone Description: Vector Backbone:pICH47742 ; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_177054 Copy
Species: Other
Genetic Insert: CrSTR, Cr7-DLH, CrLAMT, CrTDC, and CrSLS
Vector Backbone Description: Vector Backbone:pICSL4723; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments: L2 4X opattB late iridiod cytosol v2: P1_4X opattB-TMV- CrSTR-35Sterm + P2_4X opattB-TMV-Cr7DLH-masterm + P3_4X opattB-TMV-CrLAMT-ocsterm + P4_4X opattB-TMV-CrTDC-g7term + P5_4X opattB-TMV-CrSLS-nosterm
Proper citation: RRID:Addgene_177091 Copy
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