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Species: Aequorea victoria
Genetic Insert: VN155(I152L)
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3791; Vector Backbone:pMyc-CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21091444
Comments: To be published by Biotechniques in December. Please suggest the citation of our Biotechniques article. Also, this will be used as a replacement of pBiFC-VN173.
Proper citation: RRID:Addgene_27097 Copy
Species: Mus musculus
Genetic Insert: toll-like receptor 4
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:4732; Vector Backbone:p3xFLAG-CMV-8; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9851930
Proper citation: RRID:Addgene_27148 Copy
Species: Mus musculus
Genetic Insert: toll-like receptor 4(Lps)
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:4732; Vector Backbone:p3xFLAG-CMV-8; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9851930
Proper citation: RRID:Addgene_27149 Copy
Species: Homo sapiens
Genetic Insert: CK2alpha
Vector Backbone Description: Backbone Marker:GE Healthcare Life Sciences; Backbone Size:4952; Vector Backbone:pGEX-3X; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21036246
Proper citation: RRID:Addgene_27083 Copy
Genetic Insert: eGFP
Vector Backbone Description: Backbone Size:10180; Vector Backbone:pCXLE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21460823
Proper citation: RRID:Addgene_27082 Copy
Species: Homo sapiens
Genetic Insert: L-MYC
Vector Backbone Description: Backbone Size:10180; Vector Backbone:pCXLE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21460823
Proper citation: RRID:Addgene_27080 Copy
Genetic Insert: MS2 binding sites (6)
Vector Backbone Description: Backbone Marker:Pharmacia; Backbone Size:3422; Vector Backbone:pSL1180; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9809065
Comments: The Singer Lab no longer recommends this plasmid, as it has a higher rate of bacterial recombination.
For mammalian cells they recommend the following plasmids:
pUbC-FLAG-24xSuntagV4-oxEBFP-AID-baUTR1-24xMS2V5-Wpre (Plasmid #84561)
HaloTag-bActinCDS-bActinUTR-MS2V5 (Plasmid #102718)
For yeast cells they recommend the following plasmids:
pET246-pUC57 12xMS2V6 (Plasmid #104390)
pET259-pUC57 24xMS2V6 (Plasmid #104391)
pET251-pUC 12xMS2V6 Loxp KANr Loxp (Plasmid #104392)
pET264-pUC 24xMS2V6 Loxp KANr Loxp (Plasmid #104393)
Loop sequence: acatgaggatcacccatgt
Addgene's sequencing result contains 1 nucleotide mismatch at bp# 345 when compared to the full plasmid sequence provided by the depositing laboratory.
Proper citation: RRID:Addgene_27118 Copy
Species: Homo sapiens
Genetic Insert: Smad3
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCS2; Vector Types:xenopus/mammalian/avian/zebrafish; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19917253
Proper citation: RRID:Addgene_27114 Copy
Species: Homo sapiens
Genetic Insert: SOX2, KLF4
Vector Backbone Description: Backbone Size:10180; Vector Backbone:pCXLE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21460823
Proper citation: RRID:Addgene_27078 Copy
Species: Homo sapiens
Genetic Insert: Smad3
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCS2; Vector Types:xenopus/mammalian/avian/zebrafish; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19917253
Comments: A204 was mutated back to the WT residue on the original pCS2-Flag Smad3 EPSM plasmid.
Proper citation: RRID:Addgene_27111 Copy
Species: Homo sapiens
Genetic Insert: C-MYC, LIN28, NANOG
Vector Backbone Description: Backbone Size:10180; Vector Backbone:pCXLE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21460823
Proper citation: RRID:Addgene_27079 Copy
Species: Homo sapiens
Genetic Insert: OCT3/4
Vector Backbone Description: Backbone Size:10180; Vector Backbone:pCXLE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21460823
Proper citation: RRID:Addgene_27076 Copy
Species: Homo sapiens
Genetic Insert: Smad3
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4900; Vector Backbone:pGEX-6P; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19917253
Comments: A213 was mutated back to the wild type residue in EPSM construct
Proper citation: RRID:Addgene_27110 Copy
Species: Homo sapiens
Genetic Insert: CK2beta
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4358; Vector Backbone:pBI; Vector Types:Mammalian Expression, tetracycline-regulated expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21036246
Proper citation: RRID:Addgene_27094 Copy
Species: Homo sapiens
Genetic Insert: CK2beta
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4358; Vector Backbone:pBI; Vector Types:Mammalian Expression, tetracycline-regulated expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21036246
Proper citation: RRID:Addgene_27095 Copy
Species: Homo sapiens
Genetic Insert: CK2alpha' K69M
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5542; Vector Backbone:pRC/CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21036246
Proper citation: RRID:Addgene_27090 Copy
Species: E. coli
Genetic Insert: Colicin Ia + Ia immunity protein
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5708; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11004207
Comments: This plasmid contains the C-terminal channel forming domain of colicin Ia and the Ia immunity protein. The construct is also referred to as "CT-Medium or CT-M".
The SOS promoter for colicin expression is at around residues 148-165 of the insert, in the forward direction. The immunity promoter -35 box is about 460 bp from the end of the insert; there are only about 20 bp between the terminator for the colicin gene, reading in one direction, and the terminator for the immunity protein, reading in the other.
Proper citation: RRID:Addgene_27126 Copy
Species: Mycobacterium tuberculosis
Genetic Insert: PanB
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pGMCS; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:20711362
Proper citation: RRID:Addgene_27127 Copy
Species: Homo sapiens
Genetic Insert: beta-actin
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17030983
Comments: Note: Addgene's sequencing results indicate the presence of several nucleotide inserts (starting at bp# 2459, 2501 and 2516) when compared to the sequence provided by the depositing laboratory. The depositing scientist confirmed that our sequencing result is correct and the inserts do not affect the function of the plasmid.
Proper citation: RRID:Addgene_27124 Copy
Species: Homo sapiens
Genetic Insert: CK2alpha'
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5542; Vector Backbone:pRC/CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21036246
Proper citation: RRID:Addgene_27087 Copy
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