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Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 nsp7
Vector Backbone Description: Backbone Size:6080; Vector Backbone:pTwist_CMV_Puro_6 with AarI site removed; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: NCBI reference sequence: 43740578
Proper citation: RRID:Addgene_153715 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 nsp11
Vector Backbone Description: Backbone Size:6525; Vector Backbone:pTwist_CMV_Hygro_6 with AarI site removed; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: NCBI reference sequence: 43740578
Proper citation: RRID:Addgene_153672 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 ORF6 protein
Vector Backbone Description: Backbone Size:6525; Vector Backbone:pTwist_CMV_Hygro_6 with AarI site removed; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: NCBI reference sequence: 43740572
Proper citation: RRID:Addgene_153675 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 E (envelope)
Vector Backbone Description: Backbone Size:6525; Vector Backbone:pTwist_CMV_Hygro_6 with AarI site removed; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: NCBI reference sequence: 43740570
Proper citation: RRID:Addgene_153681 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 ORF7b
Vector Backbone Description: Backbone Size:6525; Vector Backbone:pTwist_CMV_Hygro_6 with AarI site removed; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: NCBI reference sequence: 43740574
Proper citation: RRID:Addgene_153680 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: Nsp13_SARS2
Vector Backbone Description: Backbone Marker:Cheryl Arrowsmith, Addgene Plasmid #62304; Vector Backbone:pFBOH-MHL; Vector Types:Baculovirus Expresssion; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_234374 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: Nsp12_SARS2
Vector Backbone Description: Vector Backbone:pFBD-BirA; Vector Types:Baculovirus Expresssion; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_234377 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: 6WEY
Vector Backbone Description: Backbone Size:5679; Vector Backbone:pET29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: 5' cloning site: BsaI (destroyed), 3' cloning site: BsaI (destroyed). Replicase polyprotein 1ab (pp1ab) (ORF1ab polyprotein) [Cleaved into Host translation inhibitor nsp1 (Leader protein) (Non-structural protein 1) (nsp1); Non-structural protein 2 (nsp2) (p65 homolog); Non-structural protein 3 (nsp3) (EC 3.4.19.12) (EC 3.4.22.-) (PL2-PRO) (Papain-like protease) (Papain-like proteinase) (PL-PRO); Non-structural protein 4 (nsp4); 3C-like proteinase (3CL-PRO) (3CLp) (EC 3.4.22.69) (Main protease) (Mpro) (Non-structural protein 5) (nsp5) (SARS coronavirus main proteinase); Non-structural protein 6 (nsp6); Non-structural protein 7 (nsp7); Non-structural protein 8 (nsp8); Non-structural protein 9 (nsp9); Non-structural protein 10 (nsp10) (Growth factor-like peptide) (GFL); RNA-directed RNA polymerase (Pol) (RdRp) (EC 2.7.7.48) (Non-structural protein 12) (nsp12); Helicase (Hel) (EC 3.6.4.12) (EC 3.6.4.13) (Non-structural protein 13) (nsp13); Proofreading exoribonuclease (ExoN) (EC 3.1.13.-) (Guanine-N7 methyltransferase) (Non-structural protein 14) (nsp14); Uridylate-specific endoribonuclease (EC 3.1.-.-) (NendoU) (Non-structural protein 15) (nsp15); 2'-O-methyltransferase (EC 2.1.1.-) (Non-structural protein 16) (nsp16)]
Proper citation: RRID:Addgene_234234 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: Nsp3_SARS2
Vector Backbone Description: Vector Backbone:pNIC-Bio3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_234388 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: Nsp15-E266Q
Vector Backbone Description: Backbone Marker:purchased from GenScript; Backbone Size:5644; Vector Backbone:pET-11b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Protein sequence is MHHHHHHSSGVDLGTENLYFQSNAM + Nsp15-E266Q (amino acid residues 6453-6798 of YP_009724389, with E266Q mutation).
Confers ampicillin-resistance.
Backbone vector is pET-11b from GenScript.
Mutation design by Nirupa Nagaratnam.
Proper citation: RRID:Addgene_218803 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: Nsp15-D183N
Vector Backbone Description: Backbone Marker:purchased from GenScript; Backbone Size:5644; Vector Backbone:pET-11b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Protein sequence is MHHHHHHSSGVDLGTENLYFQSNAM + Nsp15-D183N (amino acid residues 6453-6798 of YP_009724389, with D183N mutation).
Confers ampicillin-resistance.
Backbone vector is pET-11b from GenScript.
Mutation design by Nirupa Nagaratnam.
Proper citation: RRID:Addgene_219526 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: Nsp15-A81V
Vector Backbone Description: Backbone Marker:purchased from GenScript; Backbone Size:5644; Vector Backbone:pET-11b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Protein sequence is MHHHHHHSSGVDLGTENLYFQSNAM + Nsp15-A81V (amino acid residues 6453-6798 of YP_009724389, with A81V mutation).
Confers ampicillin-resistance.
Backbone vector is pET-11b from GenScript.
Mutation design by Nirupa Nagaratnam.
Proper citation: RRID:Addgene_219525 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: Nsp15-V66L
Vector Backbone Description: Backbone Marker:purchased from GenScript; Backbone Size:5644; Vector Backbone:pET-11b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Protein sequence is MHHHHHHSSGVDLGTENLYFQSNAM + Nsp15-V66L (amino acid residues 6453-6798 of YP_009724389, with V66L mutation).
Confers ampicillin-resistance.
Backbone vector is pET-11b from GenScript.
Mutation design by Nirupa Nagaratnam.
Proper citation: RRID:Addgene_219524 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 5' UTR SL1 alone
Vector Backbone Description: Vector Backbone:pLV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_191485 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 5' UTR
Vector Backbone Description: Vector Backbone:pLV; Vector Types:Lentiviral, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_191480 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 5' UTR ΔSL1
Vector Backbone Description: Vector Backbone:pLV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_191484 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: MBP-Nsp1
Vector Backbone Description: Vector Backbone:pCMV-Dest; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_191483 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 5' UTR SL1 alone
Vector Backbone Description: Vector Backbone:pLV; Vector Types:Lentiviral, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_191482 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 5' UTR ΔSL1
Vector Backbone Description: Vector Backbone:pLV; Vector Types:Lentiviral, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_191481 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: S-GSAS-OMICRON.BA.2
Vector Backbone Description: Backbone Size:4745; Vector Backbone:pαH; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.04.07.487528v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_184829 Copy
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