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Species: Homo sapiens
Genetic Insert: shREV3-4
Vector Backbone Description: Backbone Marker:Trono Laboratory; Backbone Size:11085; Vector Backbone:pLVTHM; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
References:
Comments: A versatile tool for conditional gene expression and knockdown.
Szulc J, Wiznerowicz M, Sauvain MO, Trono D, Aebischer P.
Nat Methods. 2006 Feb;3(2):109-16.
PMID:16432520
Proper citation: RRID:Addgene_38033 Copy
Species: Homo sapiens
Genetic Insert: E2F3a
Vector Backbone Description: Backbone Marker:Vogelstein Lab ; Backbone Size:6600; Vector Backbone:pCMV-Neo-Bam; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: E2Fs bind DNA as a heterodimer with members of the DP family of transcription factors.
The 5' end of the coding sequence can be difficult to sequence.
Proper citation: RRID:Addgene_37970 Copy
Species: Homo sapiens
Genetic Insert: E2F6
Vector Backbone Description: Backbone Marker:Vogelstein Lab ; Backbone Size:6600; Vector Backbone:pCMV-Neo-Bam; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: The backbone plasmid pCMV-Neo-Bam is Addgene#16440
E2Fs bind DNA as a heterodimer with members of the DP family of transcription factors.
Proper citation: RRID:Addgene_37965 Copy
Species: Homo sapiens
Genetic Insert: DP1
Vector Backbone Description: Backbone Marker:Vogelstein Lab ; Backbone Size:6600; Vector Backbone:pCMV-Neo-Bam; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: DP1 functions as a heterodimer with several members of the E2F family of transcription factors.
Proper citation: RRID:Addgene_37968 Copy
Species: Homo sapiens
Genetic Insert: FKBP1A
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pmRFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: Compared to GenBank reference sequence NM_019892, the INPP5E insert in this plasmid contains a C641A mutation. Please see the full plasmid sequence for the most accurate information.
Proper citation: RRID:Addgene_38001 Copy
Species: Homo sapiens
Genetic Insert: YTHDF2 YTH domain family, member 2
Vector Backbone Description: Backbone Size:5496; Vector Backbone:pFRT/TO/HIS/FLAG/HA-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_38089 Copy
Species: Homo sapiens
Genetic Insert: PES1 pescadillo ribosomal biogenesis factor 1
Vector Backbone Description: Backbone Size:5490; Vector Backbone:pFRT/TO/HIS/FLAG/HA-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_38085 Copy
Species: Homo sapiens
Genetic Insert: PRDX1 peroxiredoxin 1
Vector Backbone Description: Backbone Size:5321; Vector Backbone:pFRT/TO/HIS/FLAG/HA-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_38086 Copy
Species: Homo sapiens
Genetic Insert: FKBP1A
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pmRFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: Compared to GenBank reference sequence NM_019892, the INPP5E insert in this plasmid contains a C641A mutation. Please see the full plasmid sequence for the most accurate information.
Proper citation: RRID:Addgene_38000 Copy
Species: Homo sapiens
Genetic Insert: EDF1 endothelial differentiation-related factor 1
Vector Backbone Description: Backbone Size:5496; Vector Backbone:pFRT/TO/HIS/FLAG/HA-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_38079 Copy
Species: Homo sapiens
Genetic Insert: API5 apoptosis inhibitor 5
Vector Backbone Description: Backbone Size:5496; Vector Backbone:pFRT/TO/HIS/FLAG/HA-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_38074 Copy
Species: Homo sapiens
Genetic Insert: KIAA1967
Vector Backbone Description: Backbone Size:5539; Vector Backbone:pFRT/TO/HIS/FLAG/HA-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_38082 Copy
Species: Homo sapiens
Genetic Insert: MYBBP1A MYB binding protein (P160) 1a
Vector Backbone Description: Backbone Size:5499; Vector Backbone:pFRT/TO/HIS/FLAG/HA-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_38084 Copy
Species: Homo sapiens
Genetic Insert: HNRNPR heterogeneous nuclear ribonucleoprotein R
Vector Backbone Description: Backbone Size:5538; Vector Backbone:pFRT/TO/HIS/FLAG/HA-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_38067 Copy
Species: Homo sapiens
Genetic Insert: HNRNPU heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A)
Vector Backbone Description: Backbone Size:5496; Vector Backbone:pFRT/TO/HIS/FLAG/HA-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_38068 Copy
Species: Homo sapiens
Genetic Insert: MYEF2 myelin expression factor 2
Vector Backbone Description: Backbone Size:5508; Vector Backbone:pFRT/TO/HIS/FLAG/HA-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Digest and NGS results suggest that the original Gateway entry vector is still present in the DNA stock from which the bacterial stocks are created. Because the entry vector does not contain a promoter for the insert or selection marker, it should not interfere with function.
To attempt to remove the entry vector, recipient scientists could try to cure the plasmid from isolated colonies. Alternatively, they can linearize the entry vector with BmtI, HpaI or NheI digest, which will not cut the expression vector, and retransform into bacteria.
Proper citation: RRID:Addgene_38069 Copy
Species: Homo sapiens
Genetic Insert: HNRNPD heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa)
Vector Backbone Description: Backbone Size:5496; Vector Backbone:pFRT/TO/HIS/FLAG/HA-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_38066 Copy
Species: Homo sapiens
Genetic Insert: BCR/ABL P190
Vector Backbone Description: Backbone Size:7400; Vector Backbone:pLEF; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Constructed by Suparna Mishra. Generated in a 3-way ligation consisting of 5' 0.75 kb BamHI/Sal I + 3' 6.3 kb Sal I/EcoRI BCR/ABL fragments into pLEF digested with BamHIxEcoRI. The ABL part of the insert contains the natural TAG and 3' UT sequences.
Addgene NGS identified a sequence discrepancy at nucleotide 3168 resulting in a T117M mutation in the ABL-1 sequence, relative to Genbank ID NP_005148.2 and Addgene Plasmid #38158. This is not a known polymorphism and the effect of this, if any, on the protein is not known.
Proper citation: RRID:Addgene_38159 Copy
Species: Homo sapiens
Genetic Insert: Abr
Vector Backbone Description: Backbone Marker:Don Kohn [email protected]; Backbone Size:6600; Vector Backbone:pCCL-cppt178-MNDU3; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments: Constructed by Jess Cunnick. Abr was first subcloned into pEYFP-C1 Bgl II x KpnI as a 0.4 kb 5' BamHI/BstEII + 2.2 kb BstEII- KpnI fragment. The EYFP-Abr fusion was isolated as a 5' AgeI- 3' KpnI fragment and inserted into pCCL-cppt178-MNDU3-X3 digested with Xma I x Kpn I.
Proper citation: RRID:Addgene_38155 Copy
Species: Homo sapiens
Genetic Insert: ABR
Vector Backbone Description: Backbone Size:7400; Vector Backbone:pLEF; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Constructed by Suparna Mishra. Generated in a 3-way ligation consisting of 5' 0.4 kb BamHI/BstEII + 3' 2.2 kb BstEII/EcoRI ABR fragments into pLEF digested with BamHIxEcoRI. The ABR insert contains the natural TAG and 3' UT sequences.
Addgene sequencing found an E24G point mutation--this does not alter plasmid function.
Proper citation: RRID:Addgene_38157 Copy
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