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Species: Homo sapiens
Genetic Insert: IRF-9 STAT1 C
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pCDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: This plasmid only contains the 38 C-terminal amino acids of STAT1.
Proper citation: RRID:Addgene_37543 Copy
Species: Homo sapiens
Genetic Insert: IRF-9 STAT2 C
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pCDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: C8Y point mutation in IRF9 should not affect plasmid function.
Proper citation: RRID:Addgene_37544 Copy
Species: Homo sapiens
Genetic Insert: Rabenosyn5
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: Designed and prepared by Deborah Leonard, Deanna Navaroli and Anil Chawla (Corvera Lab)
Proper citation: RRID:Addgene_37538 Copy
Species: Homo sapiens
Genetic Insert: AK3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5597; Vector Backbone:pcDNA3.1(+) hygro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_37539 Copy
Species: Homo sapiens
Genetic Insert: cysteine-free SGFP2-N
Vector Backbone Description: Backbone Marker:Clonetech; Backbone Size:4105; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_37533 Copy
Species: Homo sapiens
Genetic Insert: ss-cfSGFP2 (cysteine-free SGFP2)
Vector Backbone Description: Backbone Marker:Clonetech; Backbone Size:4105; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: This plasmid is used for expression of cfSGFP2 in the ER. The cleaved signal sequence of α1-antitrypsin (M1-K34) was fused to the N-terminus of SGFP2 using BglII and EcoRI sites. The fragment M1-A24 should be removed cotranslationally after translocation. The long flanking sequence E25-K34 of α1-antitrypsin was included to ensure access to signal peptidase
Proper citation: RRID:Addgene_37535 Copy
Species: Homo sapiens
Genetic Insert: GSK3b
Vector Backbone Description: Backbone Size:3400; Vector Backbone:pS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_37526 Copy
Species: Homo sapiens
Genetic Insert: NLRC5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_37523 Copy
Species: Homo sapiens
Genetic Insert: NLRC5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: The iso3 plasmid actually encodes the full cDNA (not only the ORF).
In comparison to the FL NLRC5, iso3 contains an insert that leads to a stop codon according to IPI00647281.1 .
Proper citation: RRID:Addgene_37510 Copy
Species: Homo sapiens
Genetic Insert: Mitogen-activated protein kinase phosphatase 7
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_37792 Copy
Species: Homo sapiens
Genetic Insert: Mitogen-activated protein kinase phosphatase 7
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_37793 Copy
Species: Homo sapiens
Genetic Insert: NUMB isoform 2
Vector Backbone Description: Backbone Marker:Beyer et al., J Virol 2002; Backbone Size:7309; Vector Backbone:M107; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_37803 Copy
Species: Homo sapiens
Genetic Insert: NUMB isoform 4
Vector Backbone Description: Backbone Marker:Beyer et al., J Virol 2002; Backbone Size:7309; Vector Backbone:M107; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_37805 Copy
Species: Homo sapiens
Genetic Insert: BAI3
Vector Backbone Description: Backbone Marker:BD Biosciences; Backbone Size:9761; Vector Backbone:pAcGP67A; Vector Types:Baculovirus Transfer Vectors; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_37848 Copy
Species: Homo sapiens
Genetic Insert: EB1
Vector Backbone Description: Vector Backbone:pShuttle; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_37829 Copy
Species: Homo sapiens
Genetic Insert: EB1
Vector Backbone Description: Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: This construct is resistant to shRNA #3.
EB1 was inserted into pEGFP-N1 via the SacI/SmaI restriction sites. The second EGFP, was then inserted via the SmaI/AgeI sites.
Proper citation: RRID:Addgene_37925 Copy
Species: Homo sapiens
Genetic Insert: EB1 shRNA #3
Vector Backbone Description: Backbone Marker:Addgene plasmid 8453; Backbone Size:7032; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_37927 Copy
Species: Homo sapiens
Genetic Insert: EB1 shRNA #1
Vector Backbone Description: Backbone Marker:Addgene plasmid 8453; Backbone Size:7032; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_37928 Copy
Species: Homo sapiens
Genetic Insert: Nucleoporin 98
Vector Backbone Description: Backbone Size:2968; Vector Backbone:pPEP-TEV; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: see also:
Strelkov, S. V., Kreplak, L., Herrmann, H. & Aebi, U. (2004). Methods Cell Biol.78, 25–43.
Proper citation: RRID:Addgene_38037 Copy
Species: Homo sapiens
Genetic Insert: shREV3-5
Vector Backbone Description: Backbone Marker:oligoengine; Backbone Size:4354; Vector Backbone:pSUPERIOR.puro; Vector Types:RNAi; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_38030 Copy
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