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Species: Homo sapiens
Genetic Insert: Synapsin I promoter
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR221 P1-P5r; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
References:
Comments: Gene Therapy 14, 872-882 (June 2007) doi:10.1038/sj.gt.3302924
Efficient gene transduction of neurons by lentivirus with enhanced neuron-specific promoters
H Hioki, H Kameda, H Nakamura, T Okunomiya, K Ohira, K Nakamura, M Kuroda, T Furuta and T Kaneko
Proper citation: RRID:Addgene_32582 Copy
Species: Homo sapiens
Genetic Insert: EDD
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4373; Vector Backbone:pCMV-Tag2B; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: Addgene NGS results identified a K503R mutation within the UBR5 translation. There is no information on the functional consequence of this mutation
Proper citation: RRID:Addgene_37188 Copy
Species: Homo sapiens
Genetic Insert: EDD
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4708; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_37190 Copy
Species: Homo sapiens
Genetic Insert: EDD C2768A
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4225; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: The depositing lab has noted that mutation K503R shouldn’t be a problem as it is outside the main functional domains. There does appear to be a relatively common SNP at K503 in Latino populations so this might be a genuine SNP-
http://exac.broadinstitute.org/variant/8-103338864-C-T
https://www.ncbi.nlm.nih.gov/projects/SNP/snp_ref.cgi?rs=rs571861064
Proper citation: RRID:Addgene_37192 Copy
Species: Homo sapiens
Genetic Insert: A2BR
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: A2B was PCR amplified as a 1068bp fragment incorporating a 5' EcoRI site. The stop codon was eliminated and a 3' BamHI site was added with the reverse PCR primer to allow direct cloning into pEYFP-N1
Proper citation: RRID:Addgene_37202 Copy
Species: Homo sapiens
Genetic Insert: lig4
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4776; Vector Backbone:pFASTBAC1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_37150 Copy
Species: Homo sapiens
Genetic Insert: XRCC6
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4776; Vector Backbone:pFASTBAC1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_37148 Copy
Species: Homo sapiens
Genetic Insert: XRCC5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4776; Vector Backbone:pFASTBAC1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_37149 Copy
Species: Homo sapiens
Genetic Insert: vang-like 2
Vector Backbone Description: Backbone Size:4224; Vector Backbone:pAdLox GI 1488684; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Ampicillin
References:
Comments: contains EGFP hVangl2 WT
Proper citation: RRID:Addgene_37257 Copy
Species: Homo sapiens
Genetic Insert: Scribble
Vector Backbone Description: Backbone Size:4224; Vector Backbone:pAdLox GI 1488684; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: contains EGFP C-term
Proper citation: RRID:Addgene_37256 Copy
Species: Homo sapiens
Genetic Insert: Scribble
Vector Backbone Description: Backbone Size:4224; Vector Backbone:pAdLox GI 1488684; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: contains EGFP-95-1630 hScrib
Proper citation: RRID:Addgene_37252 Copy
Species: Homo sapiens
Genetic Insert: Mff1
Vector Backbone Description: Backbone Marker:OligoEngine; Backbone Size:6350; Vector Backbone:pSuper-Retro-Puro; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_37247 Copy
Species: Homo sapiens
Genetic Insert: Drp1
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4973; Vector Backbone:pGEX-5X2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Addgene sequencing results show a V638F mutation in pGEX-Drp1. Please note that this is a polymorphism that does not affect function.
Proper citation: RRID:Addgene_37244 Copy
Species: Homo sapiens
Genetic Insert: RalBP1
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4973; Vector Backbone:pGEX-5X2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_37245 Copy
Species: Homo sapiens
Genetic Insert: HPV45L1+L2
Vector Backbone Description: Vector Backbone:custom; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_37323 Copy
Species: Homo sapiens
Genetic Insert: HPV58L1+L2
Vector Backbone Description: Vector Backbone:custom; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_37324 Copy
Species: Homo sapiens
Genetic Insert: HsNot7
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4769; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_37325 Copy
Species: Homo sapiens
Genetic Insert: SEAP
Vector Backbone Description: Vector Backbone:custom; Vector Types:Mammalian Expression; Bacterial Resistance:Blasticidin
References:
Comments:
Proper citation: RRID:Addgene_37326 Copy
Species: Homo sapiens
Genetic Insert: HPV31L1+L2
Vector Backbone Description: Vector Backbone:custom; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_37322 Copy
Species: Homo sapiens
Genetic Insert: Ran
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: Please note that Addgene's sequencing result exactly matches the full plasmid sequence provided by the depositing laboratory; however, when these sequences are compared to GenBank ID NP_006316.1 there appears to be a T24N mutation.
Proper citation: RRID:Addgene_37396 Copy
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