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Species: Synthetic
Genetic Insert: Abe1 RT, Eco1 ncRNA, MspRecT
Vector Backbone Description: Backbone Marker:Graham Hatfull (Addgene #26910); Vector Backbone:pJV62; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:42026131
Comments: Please visit https://doi.org/10.1101/2025.06.16.660010 for bioRxiv preprint.
Proper citation: RRID:Addgene_240708 Copy
Species: Synthetic
Genetic Insert: Kva1 RT, Eco1 ncRNA, MspRecT
Vector Backbone Description: Backbone Marker:Graham Hatfull (Addgene #26910); Vector Backbone:pJV62; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:42026131
Comments: Please visit https://doi.org/10.1101/2025.06.16.660010 for bioRxiv preprint.
Proper citation: RRID:Addgene_240709 Copy
Species: Synthetic
Genetic Insert: lifeact-mNeongreen
Vector Backbone Description: Vector Backbone:pU6a:sgRNA#58; Vector Types:Mini-Golden; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:41630603
Proper citation: RRID:Addgene_241206 Copy
Species: Synthetic
Genetic Insert: lifeact-mNeongreen
Vector Backbone Description: Vector Backbone:pU6a:sgRNA#57; Vector Types:Mini-Golden; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:41630603
Proper citation: RRID:Addgene_241205 Copy
Genetic Insert: pSC101-sfGFP
Vector Backbone Description: Vector Backbone:p15a, CamR, phagemid; Vector Types:Bacterial Expression, Synthetic Biology, phagemid; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:35150576
Proper citation: RRID:Addgene_241969 Copy
Species: Bacillus licheniformis
Genetic Insert: Tagatose pathway
Vector Backbone Description: Vector Backbone:p15a, CamR, phagemid; Vector Types:Bacterial Expression, Synthetic Biology, phagemid; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:35150576
Proper citation: RRID:Addgene_241968 Copy
Species: Synthetic
Genetic Insert: mNeongreen
Vector Backbone Description: Vector Backbone:pU6a:sgRNA#23; Vector Types:Mini-Golden; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:41630603
Proper citation: RRID:Addgene_241171 Copy
Species: Synthetic
Genetic Insert: mNeongreen
Vector Backbone Description: Vector Backbone:pU6a:sgRNA#25; Vector Types:Mini-Golden; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:41630603
Proper citation: RRID:Addgene_241173 Copy
Species: Synthetic
Genetic Insert: mNeongreen
Vector Backbone Description: Vector Backbone:pU6a:sgRNA#28; Vector Types:Mini-Golden; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:41630603
Proper citation: RRID:Addgene_241176 Copy
Species: Synthetic
Genetic Insert: mNeongreen
Vector Backbone Description: Vector Backbone:pU6a:sgRNA#30; Vector Types:Mini-Golden; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:41630603
Proper citation: RRID:Addgene_241178 Copy
Species: Synthetic
Genetic Insert: mNeongreen
Vector Backbone Description: Vector Backbone:pU6a:sgRNA#29; Vector Types:Mini-Golden; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:41630603
Proper citation: RRID:Addgene_241177 Copy
Species: Synthetic
Genetic Insert: mScarlet-I
Vector Backbone Description: Backbone Marker:Baker et al., 1999; Backbone Size:4108; Vector Backbone:pCS107; Vector Types:Vertebrate gene expression and general in vitro transcription; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_240641 Copy
Genetic Insert: vioABCDE
Vector Backbone Description: Vector Backbone:p15a, CamR, coi(Y35H) phagemid; Vector Types:Bacterial Expression, Synthetic Biology, phagemid; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:35150576
Proper citation: RRID:Addgene_241972 Copy
Species: Synthetic
Genetic Insert: mTurquoise2
Vector Backbone Description: Backbone Marker:Baker et al., 1999; Backbone Size:4108; Vector Backbone:pCS107; Vector Types:Vertebrate gene expression and general in vitro transcription.; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_240640 Copy
Species: Synthetic
Genetic Insert: acry-mCherry
Vector Backbone Description: Vector Backbone:pU6a:sgRNA#31; Vector Types:Mini-Golden; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:41630603
Proper citation: RRID:Addgene_241179 Copy
Species: Exaiptasia diaphana
Genetic Insert: AIPGENE865 promoter
Vector Backbone Description: Backbone Marker:Tim Howes; Backbone Size:3854; Vector Backbone:pT2HE; Vector Types:Gene expression and genomic integration in fish; Bacterial Resistance:Ampicillin
Comments: The whole intergenic region between AIPGENE865 (KXJ12416.1; Elongation Factor 1alpha) and AIPGENE899 was cloned as promoter fragment. The original gene annotation for AIPGENE865 is incorrect, the automatic NCBI annotation is correct in regards of exons, introns and translation start site. We found that Aiptasia strain CC7 was actually heterozygous for that locus: one allele was mostly in agreement with the genome sources but the other one had a couple of SNPs and a large, 749-bp insert in the intergenic region. The longer version with insert was used as promoter for this plasmid.
The Aiptasia MEK2 locus was found to be erroneously split into AIPGENES3280 & 23794 on two different scaffolds due to an assembly gap in the Baumgarten et al., 2015 genome. Correct CDS was identified from sequencing based on cDNA of strain CC7.
AmNeonGreen is a version of mNeonGreen codon-optimized for Aiptasia (based on the transcriptome of Lehnert et al., 2014) with the identical protein sequence.
Baumgarten S, Simakov O, Esherick LY, Liew YJ, Lehnert EM, Michell CT, Li Y, Hambleton EA, Guse A, Oates ME, et al. 2015. The genome of Aiptasia, a sea anemone model for coral symbiosis. Proc Natl Acad Sci. 112(38):11893–11898. doi:10.1073/pnas.1513318112.
Lehnert EM, Mouchka ME, Burriesci MS, Gallo ND, Schwarz JA, Pringle JR. 2014. Extensive differences in gene expression between symbiotic and aposymbiotic cnidarians. G3 Bethesda Md. 4(2):277–295. doi:10.1534/g3.113.009084.
Proper citation: RRID:Addgene_240643 Copy
Species: Exaiptasia diaphana
Genetic Insert: AIPGENE865 promoter
Vector Backbone Description: Backbone Marker:Tim Howes; Backbone Size:3366; Vector Backbone:pT2HE; Vector Types:Gene expression and genomic integration in fish; Bacterial Resistance:Ampicillin
Comments: The whole intergenic region between AIPGENE865 (KXJ12416.1; Elongation Factor 1alpha) and AIPGENE899 was cloned as promoter fragment. The original gene annotation for AIPGENE865 is incorrect, the automatic NCBI annotation is correct in regards of exons, introns and translation start site. We found that Aiptasia strain CC7 was actually heterozygous for that locus: one allele was mostly in agreement with the genome sources but the other one had a couple of SNPs and a large, 749-bp insert in the intergenic region. The longer version with insert was used as promoter for this plasmid.
AmNeonGreen is a version of mNeonGreen codon-optimized for Aiptasia (based on the transcriptome of Lehnert et al., 2014) with the identical protein sequence.
Lehnert EM, Mouchka ME, Burriesci MS, Gallo ND, Schwarz JA, Pringle JR. 2014. Extensive differences in gene expression between symbiotic and aposymbiotic cnidarians. G3 Bethesda Md. 4(2):277–295. doi:10.1534/g3.113.009084.
Proper citation: RRID:Addgene_240644 Copy
Species: Synthetic
Genetic Insert: Eco2 RT, Eco2 ncRNA, CspRecT and EcSSB
Vector Backbone Description: Backbone Marker:George Church (Addgene #138474); Vector Backbone:pORTMAGE-Ec1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:42026131
Comments: Please visit https://doi.org/10.1101/2025.06.16.660010 for bioRxiv preprint.
Proper citation: RRID:Addgene_240692 Copy
Species: E. coli
Genetic Insert: dnaQ
Vector Backbone Description: Vector Backbone:pCDFDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Proper citation: RRID:Addgene_241263 Copy
Species: E. coli
Genetic Insert: FLAG-S6-dnaB
Vector Backbone Description: Vector Backbone:pET(3c); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_241268 Copy
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