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  • Organism:homo sapiens (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pSS2 (hS100B)
 
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1+ mentions
RRID:Addgene_26774 S100B Homo sapiens Ampicillin PMID:8688416 There are a few mismatches between author sequence and Addgene quality control sequence. These nucleotide differences do not alter the amino acid sequence or the function of the plasmid. Backbone Marker:Stan Dunn; Backbone Size:5696; Vector Backbone:pSD80; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-07-25 12:45:12 2
EMTB-3XGFP
 
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10+ mentions
RRID:Addgene_26741 ensconsin Homo sapiens Ampicillin PMID:19060892 The microtubule binding domain of ensconsin (EMTB) was fused to 3 GFP molecules in order to visualize microtubules. For cloning details see Faire et al., 1999 Journal of Cell Science 112, 4243-4255. Note: It is difficult to sequence entirely through 3 tandem GFPs. Users are encouraged to perform a double-digest on this plasmid with EcoRI and XhoI to verify the presence of a ~3kb band corresponding to the EMTB insert + 3GFPs. Please see above attachment. Backbone Size:4097; Vector Backbone:pCS2+; Vector Types:Mammalian Expression, probe for microtubules; Bacterial Resistance:Ampicillin Only contains the microtubule binding-domain of ensconsin (amino acids 18-283 of GenBank reference sequence X73882) 2026-07-25 12:45:11 33
pcDNA3.3_LIN28A
 
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RRID:Addgene_26819 5'UTR-LIN28A-3'UTR Homo sapiens Ampicillin PMID:20888316 Note that orientation of the insert in pcDNA does not matter -- they were cloned by TA-cloning, and either orientation is fine. Backbone Size:5400; Vector Backbone:pcDNA3.3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-07-25 12:45:12 1
pcDNA3.3_OCT4
 
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1+ mentions
RRID:Addgene_26816 5'UTR-OCT4-3'UTR Homo sapiens Ampicillin PMID:20888316 Note that OCT4 has some silent mutations. Also note that orientation of the insert in pcDNA does not matter -- they were cloned by TA-cloning, and either orientation is fine. Backbone Size:5400; Vector Backbone:pcDNA3.3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-07-25 12:45:12 4
pLv-HSA-uDys/eGFP
 
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1+ mentions
RRID:Addgene_26810 dystrophin Homo sapiens Ampicillin PMID:18511457 Human (alpha) Skeletal Actin (HSA) promotor Vp intron Backbone Size:6656; Vector Backbone:pRRL-cPPT-X-PRE-SIN; Vector Types:Lentiviral, lentiviral plasmid backbone from L. Naldini; Bacterial Resistance:Ampicillin deleted R4-R23 deleted exons 71-78 2026-07-25 12:45:12 3
pCLE NICD3
 
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10+ mentions
RRID:Addgene_26894 NICD3 Homo sapiens Ampicillin PMID:16508304 EF1a xenopus enhancer-promoter-hNICD3-IRES-AlkPhos A1757T mutation in Notch3 is not known to affect function. Backbone Size:0; Vector Backbone:pCLE; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin 2026-07-25 12:45:13 13
pAAV-Ef1a-DIO eNpHR 3.0-EYFP
 
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50+ mentions
RRID:Addgene_26966 eNpHR 3.0 Homo sapiens Ampicillin PMID:20303157 This plasmid contains the human elongation factor-1a promoter. Golgi export trafficking signal (TS) sequence is KSRITSEGEYIPLDQIDINV. ER export sequence is FCYENEV. For additional information please visit - http://www.optogenetics.org Backbone Size:5609; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin ER export at 3' end of gene. Trafficking signal (TS) in between gene and fluorophore. 2026-07-25 12:45:13 81
pAAV-Ef1a-DIO ChETA-EYFP
 
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10+ mentions
RRID:Addgene_26968 hChR2(E123T/H134R)-EYFP Homo sapiens Ampicillin PMID:20081849 This plasmid contains the human elongation factor-1a promoter. For additional information please visit - http://www.optogenetics.org Backbone Marker:Invitrogen; Backbone Size:5587; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin E123T, H134R 2026-07-25 12:45:13 18
pRS-puro shNEDD4L #1
 
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1+ mentions
RRID:Addgene_27016 shRNA NEDD4L #1 Homo sapiens Ampicillin PMID:19917253 shRNA sequence - 5'-gatccccGCTAGACTGTGGATTGAGTttcaagagaACTCAATCCACAGTCTAGCtttttggaaa Backbone Size:6401; Vector Backbone:pRetroSuper; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin 2026-07-25 12:45:14 3
TAp63gamma-(3X)HA
 
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1+ mentions
RRID:Addgene_26977 TAp63gamma Homo sapiens Ampicillin PMID:17399686 Backbone Marker:Invitrogen; Backbone Size:5597; Vector Backbone:pcDNA3.1 Hygro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-07-25 12:45:13 2
pAAV-hSyn-hChR2(H134R)-mCherry
 
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10+ mentions
RRID:Addgene_26976 hChR2(H134R) Homo sapiens Ampicillin This plasmid contains the human synapsin I promoter. For additional information please visit - http://www.optogenetics.org Backbone Marker:Invitrogen; Backbone Size:4568; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin H134R 2026-07-25 12:45:13 23
pAAV-hSyn-hChR2(H134R)-EYFP
 
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100+ mentions
RRID:Addgene_26973 hChR2(H134R) Homo sapiens Ampicillin This plasmid contains the human synapsin I promoter. For additional information please visit - http://www.optogenetics.org Backbone Marker:Invitrogen; Backbone Size:4574; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin H134R 2026-07-25 12:45:13 152
pAAV-CaMKIIa-hChR2(H134R)-mCherry
 
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10+ mentions
RRID:Addgene_26975 hChR2(H134R) Homo sapiens Ampicillin This plasmid contains the mouse calcium/calmodulin-dependent kinase II alpha subunit promoter. For additional information please visit - http://www.optogenetics.org Backbone Marker:Invitrogen; Backbone Size:5386; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin H134R 2026-07-25 12:45:13 36
pQCXIH-Flag Smad3 AAGY
 
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RRID:Addgene_27027 Smad3 Homo sapiens Ampicillin PMID:19917253 cDNA PCRed from pCS2-Flag Smad3 with 5' primer containing Flag tag. Backbone Marker:Clontech; Backbone Size:7800; Vector Backbone:pQCXIH; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin P181A P182A 2026-07-25 12:45:14 0
pQCXIH-HA NEDD4L
 
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RRID:Addgene_27028 NEDD4L Homo sapiens Ampicillin PMID:19917253 Fragment from pCS2-HA NEDD4L, fill-in to make blunt-end insert. Backbone Marker:Clontech; Backbone Size:7200; Vector Backbone:pQCXIH; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin 2026-07-25 12:45:14 0
pBABE-puro LEF1
 
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1+ mentions
RRID:Addgene_27023 LEF1 Homo sapiens Ampicillin PMID:19576624 Backbone Marker:Addgene Plasmid 1764; Backbone Size:5169; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin 2026-07-25 12:45:14 6
pLenti-CaMKIIa-eNpHR 3.0-EYFP
 
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RRID:Addgene_26970 eNpHR 3.0 Homo sapiens Ampicillin This plasmid contains the mouse calcium/calmodulin-dependent kinase II alpha subunit promoter. For additional information please visit - http://www.optogenetics.org Backbone Size:9253; Vector Backbone:pLenti; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin Trafficking Signal (TS), ER Export Signal (FCYENE) 2026-07-25 12:45:13 0
pCl-HA-YAP
 
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1+ mentions
RRID:Addgene_27007 YAP Homo sapiens Ampicillin PMID:19914168 Backbone Size:4000; Vector Backbone:pCl-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-07-25 12:45:14 2
pCI Flag Smad3 NL* EPSM
 
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RRID:Addgene_27005 Smad3 Homo sapiens Ampicillin PMID:19917253 Backbone Size:4000; Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin aa 1-203; T179V 2026-07-25 12:45:14 0
pCI HA NEDD4L S382A S468A
 
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RRID:Addgene_27003 NEDD4L Homo sapiens Ampicillin PMID:19917253 Backbone Size:4000; Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin S382A S468A 2026-07-25 12:45:14 0

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