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Species: Homo sapiens
Genetic Insert: S100B
Vector Backbone Description: Backbone Marker:Stan Dunn; Backbone Size:5696; Vector Backbone:pSD80; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: There are a few mismatches between author sequence and Addgene quality control sequence. These nucleotide differences do not alter the amino acid sequence or the function of the plasmid.
Proper citation: RRID:Addgene_26774 Copy
Species: Homo sapiens
Genetic Insert: ensconsin
Vector Backbone Description: Backbone Size:4097; Vector Backbone:pCS2+; Vector Types:Mammalian Expression, probe for microtubules; Bacterial Resistance:Ampicillin
References:
Comments: The microtubule binding domain of ensconsin (EMTB) was fused to 3 GFP molecules in order to visualize microtubules. For cloning details see Faire et al., 1999 Journal
of Cell Science 112, 4243-4255.
Note: It is difficult to sequence entirely through 3 tandem GFPs. Users are encouraged to perform a double-digest on this plasmid with EcoRI and XhoI to verify the presence of a ~3kb band corresponding to the EMTB insert + 3GFPs. Please see above attachment.
Proper citation: RRID:Addgene_26741 Copy
Species: Homo sapiens
Genetic Insert: 5'UTR-LIN28A-3'UTR
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3.3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Note that orientation of the insert in pcDNA does not matter -- they were cloned by TA-cloning, and either orientation is fine.
Proper citation: RRID:Addgene_26819 Copy
Species: Homo sapiens
Genetic Insert: 5'UTR-OCT4-3'UTR
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3.3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Note that OCT4 has some silent mutations.
Also note that orientation of the insert in pcDNA does not matter -- they were cloned by TA-cloning, and either orientation is fine.
Proper citation: RRID:Addgene_26816 Copy
Species: Homo sapiens
Genetic Insert: dystrophin
Vector Backbone Description: Backbone Size:6656; Vector Backbone:pRRL-cPPT-X-PRE-SIN; Vector Types:Lentiviral, lentiviral plasmid backbone from L. Naldini; Bacterial Resistance:Ampicillin
References:
Comments: Human (alpha) Skeletal Actin (HSA) promotor
Vp intron
Proper citation: RRID:Addgene_26810 Copy
Species: Homo sapiens
Genetic Insert: NICD3
Vector Backbone Description: Backbone Size:0; Vector Backbone:pCLE; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: EF1a xenopus enhancer-promoter-hNICD3-IRES-AlkPhos
A1757T mutation in Notch3 is not known to affect function.
Proper citation: RRID:Addgene_26894 Copy
Species: Homo sapiens
Genetic Insert: eNpHR 3.0
Vector Backbone Description: Backbone Size:5609; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments: This plasmid contains the human elongation factor-1a promoter. Golgi export trafficking signal (TS) sequence is KSRITSEGEYIPLDQIDINV. ER export sequence is FCYENEV.
For additional information please visit - http://www.optogenetics.org
Proper citation: RRID:Addgene_26966 Copy
Species: Homo sapiens
Genetic Insert: hChR2(E123T/H134R)-EYFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5587; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments: This plasmid contains the human elongation factor-1a promoter.
For additional information please visit - http://www.optogenetics.org
Proper citation: RRID:Addgene_26968 Copy
Species: Homo sapiens
Genetic Insert: shRNA NEDD4L #1
Vector Backbone Description: Backbone Size:6401; Vector Backbone:pRetroSuper; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
References:
Comments: shRNA sequence - 5'-gatccccGCTAGACTGTGGATTGAGTttcaagagaACTCAATCCACAGTCTAGCtttttggaaa
Proper citation: RRID:Addgene_27016 Copy
Species: Homo sapiens
Genetic Insert: TAp63gamma
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5597; Vector Backbone:pcDNA3.1 Hygro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_26977 Copy
Species: Homo sapiens
Genetic Insert: hChR2(H134R)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4568; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments: This plasmid contains the human synapsin I promoter.
For additional information please visit - http://www.optogenetics.org
Proper citation: RRID:Addgene_26976 Copy
Species: Homo sapiens
Genetic Insert: hChR2(H134R)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4574; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments: This plasmid contains the human synapsin I promoter.
For additional information please visit - http://www.optogenetics.org
Proper citation: RRID:Addgene_26973 Copy
Species: Homo sapiens
Genetic Insert: hChR2(H134R)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5386; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments: This plasmid contains the mouse calcium/calmodulin-dependent kinase II alpha subunit promoter.
For additional information please visit - http://www.optogenetics.org
Proper citation: RRID:Addgene_26975 Copy
Species: Homo sapiens
Genetic Insert: Smad3
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:7800; Vector Backbone:pQCXIH; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: cDNA PCRed from pCS2-Flag Smad3 with 5' primer containing Flag tag.
Proper citation: RRID:Addgene_27027 Copy
Species: Homo sapiens
Genetic Insert: NEDD4L
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:7200; Vector Backbone:pQCXIH; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: Fragment from pCS2-HA NEDD4L, fill-in to make blunt-end insert.
Proper citation: RRID:Addgene_27028 Copy
Species: Homo sapiens
Genetic Insert: LEF1
Vector Backbone Description: Backbone Marker:Addgene Plasmid 1764; Backbone Size:5169; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_27023 Copy
Species: Homo sapiens
Genetic Insert: eNpHR 3.0
Vector Backbone Description: Backbone Size:9253; Vector Backbone:pLenti; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments: This plasmid contains the mouse calcium/calmodulin-dependent kinase II alpha subunit promoter.
For additional information please visit - http://www.optogenetics.org
Proper citation: RRID:Addgene_26970 Copy
Species: Homo sapiens
Genetic Insert: YAP
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCl-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_27007 Copy
Species: Homo sapiens
Genetic Insert: Smad3
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_27005 Copy
Species: Homo sapiens
Genetic Insert: NEDD4L
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_27003 Copy
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