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Species: Homo sapiens
Genetic Insert: Fibrillarin
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_26673 Copy
Species: Homo sapiens
Genetic Insert: Apoptotic protease activating factor 1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5443; Vector Backbone:pET21b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_26642 Copy
Species: Homo sapiens
Genetic Insert: β3-integrin
Vector Backbone Description: Backbone Size:4800; Vector Backbone:pYFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_26653 Copy
Species: Homo sapiens
Genetic Insert: Rheb1 shRNA
Vector Backbone Description: Backbone Marker:Available at Addgene (#8453); Backbone Size:7000; Vector Backbone:pLKO.1 puro; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
References:
Comments: shRNA directed against Rheb1. Target sequence: 5'-CCTCAGACATACTCCATAGAT-3'.
Proper citation: RRID:Addgene_26625 Copy
Species: Homo sapiens
Genetic Insert: RagB shRNA
Vector Backbone Description: Backbone Marker:Available at Addgene (#8453); Backbone Size:7000; Vector Backbone:pLKO.1 puro; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
References:
Comments: shRNA directed against RagB. Target sequence: 5'-CGGGACAACATCTTCCGAAAT-3'. Sequence from the website of the RNAi consortium at the Broad institute.
Proper citation: RRID:Addgene_26627 Copy
Species: Homo sapiens
Genetic Insert: Rheb1 shRNA
Vector Backbone Description: Backbone Marker:Available at Addgene (#8453); Backbone Size:7000; Vector Backbone:pLKO.1 puro; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
References:
Comments: shRNA directed against Rheb1. Target sequence: 5'-TTATGTTGGTTGGGAATAAGA-3'.
Proper citation: RRID:Addgene_26626 Copy
Species: Homo sapiens
Genetic Insert: p18 shRNA
Vector Backbone Description: Backbone Marker:Available at Addgene (#8453); Backbone Size:7000; Vector Backbone:pLKO.1 puro; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
References:
Comments: shRNA directed against p18. Target sequence: 5'-AGACAGCCAGCAACATCATTG-3'
Proper citation: RRID:Addgene_26631 Copy
Species: Homo sapiens
Genetic Insert: raptor
Vector Backbone Description: Backbone Marker:Available at Addgene (#19319); Backbone Size:7400; Vector Backbone:pLJM1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_26633 Copy
Species: Homo sapiens
Genetic Insert: STAT3 shRNA
Vector Backbone Description: Backbone Marker:System Biosciences; Backbone Size:7200; Vector Backbone:pSIH1-H1-puro; Vector Types:Lentiviral, RNAi; Bacterial Resistance:Ampicillin
References:
Comments: STAT3 shRNA sequence: gatccGCATCTGCCTAGATCGGCTATTCAAGAGATAGCCGATCTAGGCAGATGTTTTTTg
Proper citation: RRID:Addgene_26596 Copy
Species: Homo sapiens
Genetic Insert: Rabin8
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: L196A mutant in GEF domain.
Proper citation: RRID:Addgene_26727 Copy
Species: Homo sapiens
Genetic Insert: Zyxin
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pDsRed-N1; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_26720 Copy
Species: Homo sapiens
Genetic Insert: Karyopherin Alpha 7
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4050; Vector Backbone:pCMVTNT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_26683 Copy
Species: Homo sapiens
Genetic Insert: 3x MHC I KB element - luciferase
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5600; Vector Backbone:pGL2-Basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments: The plasmid 3X-KB-L has a minimal fos promoter element and three copies of the major histocompatibility complex (MHC) class I KB element TGGGGATTCCCCA. It was constructed by ligation of the fragment obtained through digestion of MHC-NF-KB CAT by HindIII, blunting with Klenow DNA polymerase, and then digestion with BamHI. The fragment was ligated to pGL2-Basic digest(SmaI+BglII).
Proper citation: RRID:Addgene_26699 Copy
Species: Homo sapiens
Genetic Insert: Karyopherin Alpha 3
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4050; Vector Backbone:pCMVTNT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_26679 Copy
Species: Homo sapiens
Genetic Insert: Rs1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4879; Vector Backbone:pcDNA3.2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: pcDNA3.2 expression vector with the majority of the CMV promoter deleted, and carrying the EF1α-tTA/TetO-mCh-Rs1 cassette flanked by insulator sequences
Proper citation: RRID:Addgene_26797 Copy
Species: Homo sapiens
Genetic Insert: Karyopherin Alpha 1
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4050; Vector Backbone:pCMVTNT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: S73N is a reported SNP in the KPNA1 sequence. For more information, please see: https://www.ncbi.nlm.nih.gov/projects/SNP/snp_ref.cgi?type=rs&rs=4678193
Proper citation: RRID:Addgene_26677 Copy
Species: Homo sapiens
Genetic Insert: H2B-GFP
Vector Backbone Description: Backbone Marker:Bob Weinberg, Addgene plasmid 1764; Backbone Size:5200; Vector Backbone:pBabe-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: H2B-GFP was cloned from pBOS-H2B-GFP (BD Biosciences) into the pBABE retroviral vector.
Proper citation: RRID:Addgene_26790 Copy
Species: Homo sapiens
Genetic Insert: Guanidinoacetate N-methyltransferase
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5404; Vector Backbone:pGL4.20; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
References:
Comments: There are a few discrepancies between Addgene quality control sequence and NCBI reference sequences, however, these do not affect function of the plasmid.
Proper citation: RRID:Addgene_26804 Copy
Species: Homo sapiens
Genetic Insert: Tubulin
Vector Backbone Description: Backbone Marker:Agilent Technologies; Backbone Size:7469; Vector Backbone:pShuttle-CMV; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_26768 Copy
Species: Homo sapiens
Genetic Insert: eNpHR 3.0
Vector Backbone Description: Backbone Size:8433; Vector Backbone:pLenti; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments: This plasmid contains the human synapsin I promoter.
For additional information please visit - http://www.optogenetics.org
Proper citation: RRID:Addgene_26775 Copy
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