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Species: Marchantia polymorpha
Genetic Insert: MpPHOT
Vector Backbone Description: Vector Backbone:pDONR207; Vector Types:Gateway Cloning; Bacterial Resistance:Gentamicin
Defining Citation: PMID:27027881
Proper citation: RRID:Addgene_100600 Copy
Species: Arabidopsis thaliana
Genetic Insert: OEP7(1–50)-Citrine
Vector Backbone Description: Vector Backbone:pMpGWB106; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:28634853
Proper citation: RRID:Addgene_100602 Copy
Genetic Insert: hgRNA-A76
Vector Backbone Description: Vector Backbone:pLKO.1 Hygro; Vector Types:Mammalian Expression, Lentiviral, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27918539
Proper citation: RRID:Addgene_100568 Copy
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28418644
Comments: It is used to standardize new parts for EMMA toolkit. New DNA fragments (parts) are cloned into this plasmid replacing the RFP gene through a BsaI Golden Gate reaction. The resulting plasmid will have the part flanked by BsmBI sites and the fusion sites corresponding to position 8b of EMMA platform.
Proper citation: RRID:Addgene_100618 Copy
Genetic Insert: FISSEQ-hgRNA-Design1
Vector Backbone Description: Vector Backbone:pLKO.1 Hygro; Vector Types:Mammalian Expression, Lentiviral, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27918539
Proper citation: RRID:Addgene_100571 Copy
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28418644
Comments: It is used to standardize new parts for EMMA toolkit. New DNA fragments (parts) are cloned into this plasmid replacing the RFP gene through a BsaI Golden Gate reaction. The resulting plasmid will have the part flanked by BsmBI sites and the fusion sites corresponding to position 3 of EMMA platform.
Proper citation: RRID:Addgene_100611 Copy
Species: Synthetic
Genetic Insert: tLOV
Vector Backbone Description: Backbone Size:3712; Vector Backbone:pTKEI-Dest; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28707884
Proper citation: RRID:Addgene_100574 Copy
Species: E. coli
Genetic Insert: sgGal4
Vector Backbone Description: Backbone Marker:Addgene #51024; Vector Backbone:pSLQ1651-sgRNA(F+E); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28841410
Proper citation: RRID:Addgene_100549 Copy
Species: Streptococcus pyogenes
Genetic Insert: N-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9
Vector Backbone Description: Backbone Size:7064; Vector Backbone:pEF1a-FB-puro; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28841410
Proper citation: RRID:Addgene_100547 Copy
Species: Homo sapiens
Genetic Insert: thymidylate synthetase
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:5428; Vector Backbone:pCDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29100421
Proper citation: RRID:Addgene_100545 Copy
Species: Homo sapiens
Genetic Insert: thymidine kinase 1
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:5428; Vector Backbone:pCDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29100421
Proper citation: RRID:Addgene_100544 Copy
Genetic Insert: AAVS1 sgRNA
Vector Backbone Description: Vector Backbone:gRNA_cloning_vector (#41824); Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28737741
Proper citation: RRID:Addgene_100554 Copy
Genetic Insert: ciCas9(F22)
Vector Backbone Description: Backbone Marker:Life Technology; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression, CRISPR, FRT, Flp-In; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28737741
Proper citation: RRID:Addgene_100552 Copy
Genetic Insert: ciCas9(L22)
Vector Backbone Description: Backbone Marker:Life Technology; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression, CRISPR, FRT, Flp-In; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28737741
Proper citation: RRID:Addgene_100551 Copy
Genetic Insert: EMX1 sgRNA
Vector Backbone Description: Vector Backbone:gRNA_cloning_vector (#41824); Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28737741
Proper citation: RRID:Addgene_100558 Copy
Genetic Insert: MYC sgRNA5
Vector Backbone Description: Vector Backbone:gRNA_cloning_vector (#41824); Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28737741
Proper citation: RRID:Addgene_100557 Copy
Genetic Insert: MYC sgRNA4
Vector Backbone Description: Vector Backbone:gRNA_cloning_vector (#41824); Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28737741
Proper citation: RRID:Addgene_100556 Copy
Species: Synthetic
Genetic Insert: EMMA toolkit Assembly connector H-J
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28418644
Comments: DNA element with no function. It is used as a bridge to connect parts placed in position 7 and 10 of EMMA platform.
Proper citation: RRID:Addgene_100647 Copy
Species: Synthetic
Genetic Insert: EMMA toolkit Assembly connector Ha-K
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28418644
Comments: DNA element with no function. It is used as a bridge to connect parts placed in position 8a and 11 of EMMA platform.
Proper citation: RRID:Addgene_100649 Copy
Species: Synthetic
Genetic Insert: EMMA toolkit Assembly connector B-L
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28418644
Comments: DNA element with no function. It is used as a bridge to connect parts placed in position 1 and 12 of EMMA platform.
Proper citation: RRID:Addgene_100645 Copy
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