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Species: Discosoma sp.
Genetic Insert: mCherry
Vector Backbone Description: Backbone Size:10451; Vector Backbone:pYTRW07K_0G5; Vector Types:Bacterial Expression, Synthetic Biology, Yeast Expression, Tn5 genomic integration; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37333445
Comments: Part of the pYT vector series which facilitates chromosomal integration via Tn5 tranposition with selection on gentamicin and transcriptional reporter mCherry (https://www.fpbase.org/protein/mcherry/; methods: flow cytometry, fluorescence visualisation, fluorescence measurements). Target genes can be inserted at the I-SceI site.
Proper citation: RRID:Addgene_177287 Copy
Vector Backbone Description: Backbone Marker:Addgene #12254; Backbone Size:12742; Vector Backbone:pWPI; Vector Types:Mammalian Expression, Bacterial Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39251607
Comments: Please visit https://doi.org/10.1101/2023.02.27.530345 for bioRxiv preprint.
Proper citation: RRID:Addgene_214831 Copy
Species: Synthetic
Genetic Insert: Ptet-tetA(C)
Vector Backbone Description: Backbone Size:9145; Vector Backbone:yTREX backbone; Vector Types:Bacterial Expression, Synthetic Biology, Yeast Expression, rrn genomic integration; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37333445
Comments: Part of the pYT vector series which enables chromosomal interposon integration via HR in synthetic rrn "landing pads" within 16S rRNA genes of equipped strains. The vector carries the "empty" YT_core with specific cloning slots separated by designated upstream and downstream sequences for integron customization, with TcR marker. Target genes can be inserted at the I-SceI site; additional promoter or reporter elements may be integrated at further positions. The vector backbone, based on the yTREX vector, is equipped with the bacterial pMB1 ori, aphII-encoded KmR and mob gene from pBBR1 (promoter and oriT region) and with yeast replication elements CEN4-ARS1 and URA3 marker. Note: vector pYTRW0268K_0Ti1 also has SacB for counter selection.
Proper citation: RRID:Addgene_177289 Copy
Species: Shigella spc.
Genetic Insert: PaadA-aadA
Vector Backbone Description: Backbone Size:9699; Vector Backbone:pYTRW10K_0x5; Vector Types:Bacterial Expression, Synthetic Biology, Yeast Expression, Tn5 genomic integration; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37333445
Comments: Part of the pYT vector series which facilitates chromosomal integration via Tn5 tranposition with selection on streptomycin. Target genes can be inserted at the I-SceI site.
Proper citation: RRID:Addgene_177282 Copy
Species: Salmonella sp.
Genetic Insert: Ptet-tetA(C)
Vector Backbone Description: Backbone Size:9699; Vector Backbone:pYTRW10K_0x5; Vector Types:Bacterial Expression, Synthetic Biology, Yeast Expression, Tn5 genomic integration; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37333445
Comments: Part of the pYT vector series which facilitates chromosomal integration via Tn5 tranposition with selection on tetracyline. Target genes can be inserted at the I-SceI site.
Proper citation: RRID:Addgene_177281 Copy
Species: Homo sapiens
Genetic Insert: EGFP
Vector Backbone Description: Backbone Marker:VectorBuilder; Vector Backbone:Lentivirus; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36515828
Proper citation: RRID:Addgene_184593 Copy
Species: Pseudomonas aeruginosa
Genetic Insert: PaacC1-aacC1
Vector Backbone Description: Backbone Size:9699; Vector Backbone:pYTRW10K_0x5; Vector Types:Bacterial Expression, Synthetic Biology, Yeast Expression, Tn5 genomic integration; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37333445
Comments: Part of the pYT vector series which facilitates chromosomal integration via Tn5 tranposition with selection on gentamicin.
Proper citation: RRID:Addgene_177279 Copy
Species: Mus musculus
Genetic Insert: Thymosin beta 10
Vector Backbone Description: Backbone Marker:Sigma-Aldrich; Backbone Size:6279; Vector Backbone:p3xFLAG-CMV10; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36109592
Proper citation: RRID:Addgene_191133 Copy
Species: Bifidobacterium breve
Genetic Insert: M.BbrUIII
Vector Backbone Description: Backbone Size:3202; Vector Backbone:pJV170; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38936361
Proper citation: RRID:Addgene_180081 Copy
Species: Mus musculus
Genetic Insert: Anti-HA [12CA5] mouse IgG2a heavy chain
Vector Backbone Description: Backbone Marker:Institute for Protein Innovation (IPI) (Addgene plasmid # 225877); Vector Backbone:pTipi2.1; Vector Types:Mammalian Expression, Affinity Reagent/ Antibody; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_210656 Copy
Species: Mus musculus
Genetic Insert: Anti-HA [12CA5] kappa light chain
Vector Backbone Description: Backbone Marker:Institute for Protein Innovation (IPI) (Addgene plasmid # 225877); Vector Backbone:pTipi2.1; Vector Types:Mammalian Expression, Affinity Reagent/ Antibody; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_210657 Copy
Vector Backbone Description: Backbone Size:6428; Vector Backbone:pCas34; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36893454
Proper citation: RRID:Addgene_191092 Copy
Genetic Insert: mVenus
Vector Backbone Description: Backbone Marker:System Biosciences; Vector Backbone:PB533A-2; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35798028
Proper citation: RRID:Addgene_190731 Copy
Genetic Insert: nicking site to be replaced with your gene of interest
Vector Backbone Description: Backbone Size:7067; Vector Backbone:pProUSER13E3F; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34793671
Proper citation: RRID:Addgene_178026 Copy
Genetic Insert: nicking site to be replaced with your gene of interest
Vector Backbone Description: Backbone Size:6878; Vector Backbone:pProUSER13G2F; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34793671
Proper citation: RRID:Addgene_178027 Copy
Species: Homo sapiens
Genetic Insert: V5-mScarlet-FAM98B
Vector Backbone Description: Vector Backbone:pLJC5; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40674500
Proper citation: RRID:Addgene_211350 Copy
Species: Aequorea victoria
Genetic Insert: eYFP
Vector Backbone Description: Backbone Size:10418; Vector Backbone:pYTRW20K_0Ti1; Vector Types:Bacterial Expression, Synthetic Biology, Yeast Expression, rrn genomic integration; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37333445
Comments: Part of the pYT vector series which facilitates chromosomal interposon integration in rrn "landing pads" with selection on tetracyline and transcriptional reporter eYFP (https://www.fpbase.org/protein/eyfp/; methods: flow cytometry, fluorescence visualisation, fluorescence measurements). Target genes can be inserted at the I-SceI site. Note that vector pYTRW026K_1Ti1 is additionally equipped with SacB for counter selection.
Proper citation: RRID:Addgene_177291 Copy
Species: Bacillus subtilis
Genetic Insert: PsacB-sacB
Vector Backbone Description: Backbone Size:13522; Vector Backbone:pYTRW22K_7Ti1; Vector Types:Bacterial Expression, Synthetic Biology, Yeast Expression, rrn genomic integration; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37333445
Comments: Part of the pYT vector series which facilitates chromosomal interposon integration in rrn "landing pads" with selection on tetracycline and transcriptional reporter LacZ (https://www.uniprot.org/uniprot/P00722; methods: blue/ white selection, ONPG-assay). Target genes can be inserted at the I-SceI site. The vector is equipped with SacB for counter selection.
Proper citation: RRID:Addgene_177294 Copy
Species: Synthetic
Genetic Insert: smFP _Myc (from addgene #59757)
Vector Backbone Description: Backbone Marker:addgene Plasmid #135179; Vector Backbone:pAAVTRE3_Clover; Vector Types:Mammalian Expression, Bacterial Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39895625
Comments: Please visit https://doi.org/10.1101/2021.12.26.474213 for bioRxiv preprint.
Proper citation: RRID:Addgene_201208 Copy
Species: Homo sapiens
Genetic Insert: FZD6_4CRDlinker
Vector Backbone Description: Vector Backbone:pcDNA3.1 mychis; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35513706
Proper citation: RRID:Addgene_184266 Copy
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