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Species: Homo sapiens
Genetic Insert: KIAA1524
Vector Backbone Description: Backbone Size:5523; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28174209
Proper citation: RRID:Addgene_119287 Copy
Species: Synthetic
Genetic Insert: J23104MH
Vector Backbone Description: Backbone Size:3313; Vector Backbone:pSB4K5; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30819783
Comments: Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_119562 Copy
Species: Synthetic
Genetic Insert: J23111MH
Vector Backbone Description: Backbone Size:3313; Vector Backbone:pSB4K5; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30819783
Comments: Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_119563 Copy
Species: Homo sapiens
Genetic Insert: GalT
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pECFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:8670420
Comments: This construct contains amino acids 1-60 of the human galactosyltransferase. The original article describes cloning into pcDNA1 or pCDLSRa. A subsequent paper Zaal et. al. Cell 99:589-601 (1999) describes subcloning into the parent vector used here.
Addgene Note: Please see the Addgene sequencing result to view the fluorophore sequence.
Proper citation: RRID:Addgene_11937 Copy
Species: Homo sapiens
Genetic Insert: GalT
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pEYFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:8670420
Comments: This construct contains amino acids 1-60 of the human galactosyltransferase. The original article describes cloning into pcDNA1 ro pCDLSRa. A subsequent paper Zaal et. al. Cell 99:589-601 (1999) describes subcloning into the parent vector used here.
Proper citation: RRID:Addgene_11936 Copy
Species: Other
Genetic Insert: PpetE
Vector Backbone Description: Backbone Size:2247; Vector Backbone:PICH41295; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30819783
Comments: Cu2+ inducible promoter from the PetE gene. Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint.
Addgene NGS identified potential sequence discrepancies in PpetE, see Genbank file for these annotations.
Proper citation: RRID:Addgene_119572 Copy
Species: Homo sapiens
Genetic Insert: PPME1
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26499835
Proper citation: RRID:Addgene_119292 Copy
Species: Homo sapiens
Genetic Insert: Ubiquitin
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4500; Vector Backbone:EGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16606690
Comments: PAGFP is a photoactivatable version of GFP. It is essentially the same as the standard EGFP-C1 from clontech, but contains the mutations L64F/T65S/V163A/T203H, and conatins an A206K mutation to disrupt dimerization.
In total, the PAGFP-Ubiquitin insert is 983bp.
Proper citation: RRID:Addgene_11933 Copy
Species: Homo sapiens
Genetic Insert: Ubiquitin KO.G76V
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4731; Vector Backbone:EGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16606690
Proper citation: RRID:Addgene_11932 Copy
Species: Homo sapiens
Genetic Insert: Ubiquitin
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4500; Vector Backbone:EGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16702407
Proper citation: RRID:Addgene_11935 Copy
Species: Homo sapiens
Genetic Insert: Ubiquitin KO
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4731; Vector Backbone:EGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16702407
Proper citation: RRID:Addgene_11934 Copy
Species: Homo sapiens
Genetic Insert: E6-AP I
Vector Backbone Description: Backbone Size:7818; Vector Backbone:PHAGE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30257870
Proper citation: RRID:Addgene_119315 Copy
Vector Backbone Description: Vector Backbone:pICH41331; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30819783
Comments: Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_119557 Copy
Species: Homo sapiens
Genetic Insert: E6-AP I
Vector Backbone Description: Backbone Size:7818; Vector Backbone:PHAGE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30257870
Proper citation: RRID:Addgene_119316 Copy
Species: Homo sapiens
Genetic Insert: E6-AP I
Vector Backbone Description: Backbone Size:7818; Vector Backbone:PHAGE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30257870
Proper citation: RRID:Addgene_119313 Copy
Species: Rattus norvegicus
Genetic Insert: Ywhae
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5514; Vector Backbone:pcDNA3.1 His C; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11864996
Proper citation: RRID:Addgene_11942 Copy
Vector Backbone Description: Vector Backbone:pSEVA421; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30819783
Comments: Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_119555 Copy
Genetic Insert: Ub-G76V-GFP
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3970; Vector Backbone:EGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:10802622
Comments: The ubiquitin open reading frame was amplified by PCR from the Ub-Pro-Gal plasmid with the sense primer 5'-GCG GAATTCACCATGCAGATCTTCGTGAAGACT-3' and the antisense primer 5'-GCGGGATCCTGTCGACCAAGCTTC
CCCACCACACCTCTGAGACGGAGTAC-3'. The PCR product was cloned into the EcoRI and BamHI sites of the EGFP-N1 vector from Clontech.
Proper citation: RRID:Addgene_11941 Copy
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30819783
Comments: Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_119556 Copy
Species: Other
Genetic Insert: dcas9
Vector Backbone Description: Vector Backbone:R6Kgamma; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30617347
Proper citation: RRID:Addgene_119278 Copy
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